scholarly journals High resolution light-sheet based high-throughput imaging cytometry system enables visualization of intra-cellular organelles

AIP Advances ◽  
2014 ◽  
Vol 4 (9) ◽  
pp. 097125 ◽  
Author(s):  
Raju Regmi ◽  
Kavya Mohan ◽  
Partha Pratim Mondal
Keyword(s):  
High Resolution ◽  
High Throughput ◽  
Light Sheet ◽  
Imaging Cytometry ◽  
High Throughput Imaging ◽  
Cellular Organelles

scholarly journals Subvoxel light-sheet microscopy for high-resolution high-throughput volumetric imaging of large biomedical specimens

2019 ◽  
Vol 1 (01) ◽  
pp. 1 ◽  
Author(s):  
Peng Fei ◽  
Jun Nie ◽  
Juhyun Lee ◽  
Yichen Ding ◽  
Shuoran Li ◽  
...  
Keyword(s):  
High Resolution ◽  
High Throughput ◽  
Light Sheet ◽  
Volumetric Imaging ◽  
Light Sheet Microscopy

scholarly journals Sub-voxel light-sheet microscopy for high-resolution, high-throughput volumetric imaging of large biomedical specimens

2018 ◽  
Author(s):  
Peng Fei ◽  
Jun Nie ◽  
Juhyun Lee ◽  
Yichen Ding ◽  
Shuoran Li ◽  
...  
Keyword(s):  
High Resolution ◽  
High Throughput ◽  
Light Sheet ◽  
Large Field ◽  
Mouse Heart ◽  
Processing Unit ◽  
Intact Mouse ◽  
Volumetric Imaging ◽  
Light Sheet Microscopy ◽  
Wide Range

A key challenge when imaging whole biomedical specimens is how to quickly obtain massive cellular information over a large field of view (FOV). Here, we report a sub-voxel light-sheet microscopy (SLSM) method enabling high-throughput volumetric imaging of mesoscale specimens at cellular-resolution. A non-axial, continuous scanning strategy is used to rapidly acquire a stack of large-FOV images with three-dimensional (3-D) nanoscale shifts encoded. Then by adopting a sub-voxel-resolving procedure, the SLSM method models these low-resolution, cross-correlated images in the spatial domain and iteratively recovers a 3-D image with improved resolution throughout the sample. This technique can surpass the optical limit of a conventional light-sheet microscope by more than three times, with high acquisition speeds of gigavoxels per minute. As demonstrated by quick reconstruction (minutes to hours) of various samples, e.g., 3-D cultured cells, an intact mouse heart, mouse brain, and live zebrafish embryo, the SLSM method presents a high-throughput way to circumvent the tradeoff between intoto mapping of large-scale tissue (>100 mm3) and isotropic imaging of single-cell (~1-μm resolution). It also eliminates the need of complicated mechanical stitching or precisely modulated illumination, using a simple light-sheet setup and fast graphics-processing-unit (GPU)-based computation to achieve high-throughput, high-resolution 3-D microscopy, which could be tailored for a wide range of biomedical applications in pathology, histology, neuroscience, etc.

scholarly journals High-resolution, high-throughput imaging with a multibeam scanning electron microscope

2015 ◽  
Vol 259 (2) ◽  
pp. 114-120 ◽  
Author(s):  
A.L. EBERLE ◽  
S. MIKULA ◽  
R. SCHALEK ◽  
J. LICHTMAN ◽  
M.L. KNOTHE TATE ◽  
...  
Keyword(s):  
Electron Microscope ◽  
High Resolution ◽  
Scanning Electron Microscope ◽  
High Throughput ◽  
High Throughput Imaging ◽  
Scanning Electron

scholarly journals Multi-immersion open-top light-sheet microscope for high-throughput imaging of cleared tissues

2019 ◽  
Author(s):  
Adam K. Glaser ◽  
Nicholas P. Reder ◽  
Ye Chen ◽  
Chengbo Yin ◽  
Linpeng Wei ◽  
...  
Keyword(s):  
High Throughput ◽  
Light Sheet ◽  
Optical Clearing ◽  
Clinical Specimens ◽  
Light Sheet Microscopy ◽  
Clinical Laboratories ◽  
Recent Advances ◽  
Large Numbers ◽  
Molecular Information ◽  
High Throughput Imaging

AbstractRecent advances in optical clearing and light-sheet microscopy have provided unprecedented access to structural and molecular information from intact tissues. However, current light-sheet microscopes have imposed constraints on the size, shape, number of specimens, and compatibility with various clearing protocols. Here we present a multi-immersion open-top light-sheet microscope that enables simple mounting of multiple specimens processed with a variety of protocols, which will facilitate wider adoption by preclinical researchers and clinical laboratories.One Sentence SummaryGlaseret al.describe a multi-immersion open-top light-sheet microscope that enables simple and high-throughput imaging of large numbers of preclinical and clinical specimens prepared with a variety of clearing protocols.

Abstract LB-336: High-content, high-throughput imaging cytometry for fully automated DNA damage analysis

2011 ◽  
Author(s):  
Elena Holden ◽  
Judith Newmark ◽  
Connie Wong ◽  
Maxime François ◽  
Michael Fenech ◽  
...  
Keyword(s):  
Dna Damage ◽  
High Throughput ◽  
Damage Analysis ◽  
Imaging Cytometry ◽  
High Throughput Imaging

scholarly journals Multi-immersion open-top light-sheet microscope for high-throughput imaging of cleared tissues

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Adam K. Glaser ◽  
Nicholas P. Reder ◽  
Ye Chen ◽  
Chengbo Yin ◽  
Linpeng Wei ◽  
...  
Keyword(s):  
High Throughput ◽  
Light Sheet ◽  
High Throughput Imaging
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