Market surveillance on non-halal additives incorporated in surimi based products using polymerase chain reaction (PCR)-southern hybridization analysis

2014 ◽  
Author(s):  
S. Aravindran ◽  
A. M. Sahilah ◽  
A. Aminah
Keyword(s):  
Polymerase Chain Reaction ◽  
Southern Hybridization ◽  
Hybridization Analysis ◽  
Chain Reaction ◽  
Southern Hybridization Analysis ◽  
Market Surveillance ◽  
Polymerase Chain

Sensitivity of polymerase chain reaction (PCR)-southern hybridization and conventional PCR analysis for Halal authentication of gelatin capsules

LWT ◽  
2015 ◽  
Vol 63 (1) ◽  
pp. 714-719 ◽  
Author(s):  
Sahilah Abd Mutalib ◽  
Nursheila Mustafa Muin ◽  
Aminah Abdullah ◽  
Osman Hassan ◽  
Wan Aida Wan Mustapha ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Southern Hybridization ◽  
Pcr Analysis ◽  
Conventional Pcr ◽  
Chain Reaction ◽  
Gelatin Capsules ◽  
Halal Authentication ◽  
Polymerase Chain

Isolation of an asporogenic (spoOA) protective antigen-producing strain of Bacillus anthracis

1999 ◽  
Vol 45 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Patricia L Worsham ◽  
Michele R Sowers
Keyword(s):  
Polymerase Chain Reaction ◽  
Bacillus Anthracis ◽  
Congo Red ◽  
Southern Hybridization ◽  
Protective Antigen ◽  
Essential Gene ◽  
Vaccine Production ◽  
Chain Reaction ◽  
Safe Production ◽  
Polymerase Chain

We found that Congo red agar allows identification of sporulation-deficient Bacillus anthracis. Using Congo red agar, we isolated an asporogenic derivative of the protective antigen-producing strain B. anthracis deltaSterne-1(pPA102). Polymerase chain reaction and Southern hybridization analyses of DNA from the asporogenic mutant revealed that a deletion was present in spoOA, an essential gene for the initiation of sporulation. The deletion also encompassed the spoIVB homologue and a portion of the recN homologue. The avirulent spoOA strain deltaSterne-1(pPA102)CR4 is suitable for the safe production of protective antigen without endospore contamination of the vaccine production facility.Key words: Bacillus anthracis, protective antigen, spoOA, vaccine, Congo red.

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