Site selective substitution Pt for Ti in KTiOPO4:Ga crystals revealed by electron paramagnetic resonance

2014 ◽  
Vol 116 (4) ◽  
pp. 043505 ◽  
Author(s):  
V. Grachev ◽  
M. Meyer ◽  
J. Jorgensen ◽  
A. W. Hunt ◽  
G. Malovichko
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Paramagnetic Resonance ◽  
Selective Substitution ◽  
Site Selective ◽  
Electron Paramagnetic

scholarly journals Site Selective and Efficient Spin Labeling of Proteins with a Maleimide-Functionalized Trityl Radical for Pulsed Dipolar EPR Spectroscopy

Molecules ◽  
2019 ◽  
Vol 24 (15) ◽  
pp. 2735 ◽  
Author(s):  
J. Jacques Jassoy ◽  
Caspar A. Heubach ◽  
Tobias Hett ◽  
Frédéric Bernhard ◽  
Florian R. Haege ◽  
...  
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Electron Paramagnetic Resonance Spectroscopy ◽  
Spin Labeling ◽  
Single Frequency ◽  
Spin Labels ◽  
Resonance Spectroscopy ◽  
Paramagnetic Resonance ◽  
Site Directed Spin Labeling ◽  
Site Selective ◽  
Electron Paramagnetic

Pulsed dipolar electron paramagnetic resonance spectroscopy (PDS) in combination with site-directed spin labeling (SDSL) of proteins and oligonucleotides is a powerful tool in structural biology. Instead of using the commonly employed gem-dimethyl-nitroxide labels, triarylmethyl (trityl) spin labels enable such studies at room temperature, within the cells and with single-frequency electron paramagnetic resonance (EPR) experiments. However, it has been repeatedly reported that labeling of proteins with trityl radicals led to low labeling efficiencies, unspecific labeling and label aggregation. Therefore, this work introduces the synthesis and characterization of a maleimide-functionalized trityl spin label and its corresponding labeling protocol for cysteine residues in proteins. The label is highly cysteine-selective, provides high labeling efficiencies and outperforms the previously employed methanethiosulfonate-functionalized trityl label. Finally, the new label is successfully tested in PDS measurements on a set of doubly labeled Yersinia outer protein O (YopO) mutants.

Site-selective labeling and electron paramagnetic resonance studies of human cannabinoid receptor CB2

2021 ◽  
Vol 1863 (8) ◽  
pp. 183621
Author(s):  
Alexei A. Yeliseev ◽  
Kaeli Zoretich ◽  
Levi Hooper ◽  
Walter Teague ◽  
Lioudmila Zoubak ◽  
...  
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Cannabinoid Receptor ◽  
Paramagnetic Resonance ◽  
Selective Labeling ◽  
Site Selective ◽  
Electron Paramagnetic

ELECTRON PARAMAGNETIC RESONANCE STUDIES OF PHASE TRANSITIONS IN KCN

1976 ◽  
Vol 37 (C7) ◽  
pp. C7-241-C7-246 ◽  
Author(s):  
J.P. VON DER WEID ◽  
L.C. SCAVARDA DO CARMO ◽  
R. R. DO SANTOS ◽  
B. KOILLER ◽  
S. COSTA RIBEIRO ◽  
...  
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Phase Transitions ◽  
Paramagnetic Resonance ◽  
Electron Paramagnetic
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