scholarly journals Generation of nitric oxide gradients in microfluidic devices for cell culture using spatially controlled chemical reactions

2013 ◽  
Vol 7 (6) ◽  
pp. 064104 ◽  
Author(s):  
Ying-Hua Chen ◽  
Chien-Chung Peng ◽  
Yung-Ju Cheng ◽  
Jin-Gen Wu ◽  
Yi-Chung Tung
Keyword(s):  
Nitric Oxide ◽  
Cell Culture ◽  
Chemical Reactions ◽  
Microfluidic Devices

Generation of oxygen gradients in microfluidic devices for cell culture using spatially confined chemical reactions

Lab on a Chip ◽  
2011 ◽  
Vol 11 (21) ◽  
pp. 3626 ◽  
Author(s):  
Yung-Ann Chen ◽  
Andrew D. King ◽  
Hsiu-Chen Shih ◽  
Chien-Chung Peng ◽  
Chueh-Yu Wu ◽  
...  
Keyword(s):  
Cell Culture ◽  
Chemical Reactions ◽  
Microfluidic Devices ◽  
Oxygen Gradients

Low density cell culture of locust neurons in closed-channel microfluidic devices

2010 ◽  
Vol 56 (8) ◽  
pp. 1003-1009 ◽  
Author(s):  
Katrin Göbbels ◽  
Anja Lena Thiebes ◽  
André van Ooyen ◽  
Uwe Schnakenberg ◽  
Peter Bräunig
Keyword(s):  
Cell Culture ◽  
Microfluidic Devices ◽  
Low Density ◽  
Closed Channel

scholarly journals A Modular Toolkit to Fabricate Microfluidic Devices for 3D Cell Culture and Near Real-time Measurements

2020 ◽  
Author(s):  
Giraso Kabandana ◽  
Adam Michael Ratajczak ◽  
Chengpeng Chen
Keyword(s):  
Cell Culture ◽  
Real Time ◽  
Low Cost ◽  
New Technology ◽  
Microfluidic Channel ◽  
3D Cell Culture ◽  
Microfluidic Devices ◽  
In Vitro Cell Cultures ◽  
Scoring Tools

Microfluidic technology has tremendously facilitated the development of in vitro cell cultures and studies. Conventionally, microfluidic devices are fabricated with extensive facilities by well-trained researchers, which hinders the widespread adoption of the technology for broader applications. Enlightened by the fact that low-cost microbore tubing is a natural microfluidic channel, we developed a series of adaptors in a toolkit that can twine, connect, organize, and configure the tubing to produce functional microfluidic units. Three subsets of the toolkit were thoroughly developed: the tubing and scoring tools, the flow adaptors, and the 3D cell culture suite. To demonstrate the usefulness and versatility of the toolkit, we assembled a microfluidic device and successfully applied it for 3D macrophage cultures, flow-based stimulation, and automated near real-time quantitation with new knowledge generated. Overall, we present a new technology that allows simple, fast, and robust assembly of customizable and scalable microfluidic devices with minimal facilities, which is broadly applicable to research that needs or could be enhanced by microfluidics.

scholarly journals Automation of Three-Dimensional Cell Culture in Arrayed Microfluidic Devices

2011 ◽  
Vol 16 (3) ◽  
pp. 171-185 ◽  
Author(s):  
Sara I. Montanez-Sauri ◽  
Kyung Eun Sung ◽  
John P. Puccinelli ◽  
Carolyn Pehlke ◽  
David J. Beebe
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
Microfluidic Devices ◽  
Dimensional Cell

scholarly journals Use of 3D printing and modular microfluidics to integrate cell culture, injections and electrochemical analysis

2018 ◽  
Vol 10 (27) ◽  
pp. 3364-3374 ◽  
Author(s):  
Akash S. Munshi ◽  
Chengpeng Chen ◽  
Alexandra D. Townsend ◽  
R. Scott Martin
Keyword(s):  
Nitric Oxide ◽  
Cell Culture ◽  
Endothelial Cells ◽  
3D Printing ◽  
Electrochemical Analysis ◽  
Printing Technology ◽  
3D Printing Technology

Here we show that separate modules fabricated using 3D printing technology can be easily assembled to quantitate the amount of nitric oxide released from endothelial cells following ATP stimulation.

Performing chemical reactions in virtual capillary of surface tension-confined microfluidic devices

Pramana ◽  
2005 ◽  
Vol 65 (4) ◽  
pp. 621-630 ◽  
Author(s):  
Angshuman Nag ◽  
Biswa Ranjan Panda ◽  
Arun Chattopadhyay
Keyword(s):  
Surface Tension ◽  
Chemical Reactions ◽  
Microfluidic Devices

scholarly journals A Microfluidic Chip Architecture Enabling a Hypoxic Microenvironment and Nitric Oxide Delivery in Cell Culture

Micromachines ◽  
2020 ◽  
Vol 11 (11) ◽  
pp. 979
Author(s):  
Samineh Barmaki ◽  
Daniela Obermaier ◽  
Esko Kankuri ◽  
Jyrki Vuola ◽  
Sami Franssila ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Cell Culture ◽  
Microfluidic Chip ◽  
Nitric Oxide Donor ◽  
Microfluidic Channels ◽  
Low Oxygen ◽  
Oxygen Scavenger ◽  
Hek 293 ◽  
Human Embryonic Kidney Cells ◽  
Nitric Oxide Delivery

A hypoxic (low oxygen level) microenvironment and nitric oxide paracrine signaling play important roles in the control of both biological and pathological cell responses. In this study, we present a microfluidic chip architecture for nitric oxide delivery under a hypoxic microenvironment in human embryonic kidney cells (HEK-293). The chip utilizes two separate, but interdigitated microfluidic channels. The hypoxic microenvironment was created by sodium sulfite as the oxygen scavenger in one of the channels. The nitric oxide microenvironment was created by sodium nitroprusside as the light-activated nitric oxide donor in the other channel. The solutions are separated from the cell culture by a 30 µm thick gas-permeable, but liquid-impermeable polydimethylsiloxane membrane. We show that the architecture is preliminarily feasible to define the gaseous microenvironment of a cell culture in the 100 µm and 1 mm length scales.

Micro- and nanometer-scale patterned surface in a microchannel for cell culture in microfluidic devices

2007 ◽  
Vol 390 (3) ◽  
pp. 817-823 ◽  
Author(s):  
Makiko Goto ◽  
Takehiko Tsukahara ◽  
Kiichi Sato ◽  
Takehiko Kitamori
Keyword(s):  
Cell Culture ◽  
Microfluidic Devices ◽  
Nanometer Scale ◽  
Patterned Surface
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