scholarly journals Discrimination between the human prostate normal cell and cancer cell by using a novel electrical impedance spectroscopy controlling the cross-sectional area of a microfluidic channel

2013 ◽  
Vol 7 (4) ◽  
pp. 044126 ◽  
Author(s):  
Giseok Kang ◽  
Young-jae Kim ◽  
Hong-sang Moon ◽  
Jeong-Woo Lee ◽  
Tag-Keun Yoo ◽  
...  
Keyword(s):  
Impedance Spectroscopy ◽  
Cancer Cell ◽  
Normal Cell ◽  
Electrical Impedance ◽  
Microfluidic Channel ◽  
Cross Sectional Area ◽  
Electrical Impedance Spectroscopy ◽  
Human Prostate ◽  
Sectional Area ◽  
Cross Sectional

Estimating myofiber cross‐sectional area and connective tissue deposition with electrical impedance myography: a study in D2 ‐mdx mice

2021 ◽  
Author(s):  
Sarbesh R. Pandeya ◽  
Janice A. Nagy ◽  
Daniela Riveros ◽  
Carson Semple ◽  
Rebecca S. Taylor ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Electrical Impedance ◽  
Cross Sectional Area ◽  
Mdx Mice ◽  
Sectional Area ◽  
Cross Sectional

scholarly journals Predicting myofiber cross‐sectional area and triglyceride content with electrical impedance myography: A study in db/db mice

2020 ◽  
Vol 63 (1) ◽  
pp. 127-140
Author(s):  
Sarbesh R. Pandeya ◽  
Janice A. Nagy ◽  
Daniela Riveros ◽  
Carson Semple ◽  
Rebecca S. Taylor ◽  
...  
Keyword(s):  
Electrical Impedance ◽  
Cross Sectional Area ◽  
Sectional Area ◽  
Cross Sectional ◽  
Triglyceride Content

scholarly journals Electrical Impedance Spectroscopy for Monitoring Chemoresistance of Cancer Cells

Micromachines ◽  
2020 ◽  
Vol 11 (9) ◽  
pp. 832
Author(s):  
Lexi Crowell ◽  
Juan Yakisich ◽  
Brian Aufderheide ◽  
Tayloria Adams
Keyword(s):  
Impedance Spectroscopy ◽  
Cancer Cells ◽  
Cancer Cell ◽  
Cellular Response ◽  
Electrical Impedance ◽  
Electrical Impedance Spectroscopy ◽  
Cell Behavior ◽  
Drug Resistant ◽  
Cancerous Cells

Electrical impedance spectroscopy (EIS) is an electrokinetic method that allows for the characterization of intrinsic dielectric properties of cells. EIS has emerged in the last decade as a promising method for the characterization of cancerous cells, providing information on inductance, capacitance, and impedance of cells. The individual cell behavior can be quantified using its characteristic phase angle, amplitude, and frequency measurements obtained by fitting the input frequency-dependent cellular response to a resistor–capacitor circuit model. These electrical properties will provide important information about unique biomarkers related to the behavior of these cancerous cells, especially monitoring their chemoresistivity and sensitivity to chemotherapeutics. There are currently few methods to assess drug resistant cancer cells, and therefore it is difficult to identify and eliminate drug-resistant cancer cells found in static and metastatic tumors. Establishing techniques for the real-time monitoring of changes in cancer cell phenotypes is, therefore, important for understanding cancer cell dynamics and their plastic properties. EIS can be used to monitor these changes. In this review, we will cover the theory behind EIS, other impedance techniques, and how EIS can be used to monitor cell behavior and phenotype changes within cancerous cells.

scholarly journals Dielectrophoretic and Electrical Impedance Differentiation of Cancerous Cells Based on Biophysical Phenotype

Biosensors ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 401
Author(s):  
Ina Turcan ◽  
Iuliana Caras ◽  
Thomas Gabriel Schreiner ◽  
Catalin Tucureanu ◽  
Aurora Salageanu ◽  
...  
Keyword(s):  
Impedance Spectroscopy ◽  
Tumor Cells ◽  
Cancer Cell ◽  
Primary Tumor ◽  
Electrical Impedance ◽  
Mononuclear Cells ◽  
Equivalent Circuit Model ◽  
Electrical Impedance Spectroscopy ◽  
Label Free ◽  
Peripheral Blood Mononuclear

Here, we reported a study on the detection and electrical characterization of both cancer cell line and primary tumor cells. Dielectrophoresis (DEP) and electrical impedance spectroscopy (EIS) were jointly employed to enable the rapid and label-free differentiation of various cancer cells from normal ones. The primary tumor cells that were collected from two colorectal cancer patients and cancer cell lines (SW-403, Jurkat, and THP-1), and healthy peripheral blood mononuclear cells (PBMCs) were trapped first at the level of interdigitated microelectrodes with the help of dielectrophoresis. Correlation of the cells dielectric characteristics that was obtained via electrical impedance spectroscopy (EIS) allowed evident differentiation of the various types of cell. The differentiations were assigned to a “dielectric phenotype” based on their crossover frequencies. Finally, Randles equivalent circuit model was employed for highlighting the differences with regard to a series group of charge transport resistance and constant phase element for cancerous and normal cells.

scholarly journals SIRT1–NOX4 signaling axis regulates cancer cachexia

2020 ◽  
Vol 217 (7) ◽  
Author(s):  
Aneesha Dasgupta ◽  
Surendra K. Shukla ◽  
Enza Vernucci ◽  
Ryan J. King ◽  
Jaime Abrego ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Cancer Patients ◽  
Cancer Cell ◽  
Muscle Fiber ◽  
Cross Sectional Area ◽  
Therapeutic Interventions ◽  
Sectional Area ◽  
Cross Sectional ◽  
Sirt1 Expression ◽  
Nadph Oxidase 4

Approximately one third of cancer patients die due to complexities related to cachexia. However, the mechanisms of cachexia and the potential therapeutic interventions remain poorly studied. We observed a significant positive correlation between SIRT1 expression and muscle fiber cross-sectional area in pancreatic cancer patients. Rescuing Sirt1 expression by exogenous expression or pharmacological agents reverted cancer cell–induced myotube wasting in culture conditions and mouse models. RNA-seq and follow-up analyses showed cancer cell–mediated SIRT1 loss induced NF-κB signaling in cachectic muscles that enhanced the expression of FOXO transcription factors and NADPH oxidase 4 (Nox4), a key regulator of reactive oxygen species production. Additionally, we observed a negative correlation between NOX4 expression and skeletal muscle fiber cross-sectional area in pancreatic cancer patients. Knocking out Nox4 in skeletal muscles or pharmacological blockade of Nox4 activity abrogated tumor-induced cachexia in mice. Thus, we conclude that targeting the Sirt1–Nox4 axis in muscles is an effective therapeutic intervention for mitigating pancreatic cancer–induced cachexia.

Preconcentration of Cardiac Proteins in a Cascade Microchip

2011 ◽  
Author(s):  
Mohammad R. Hossan ◽  
Talukder Z. Jubery ◽  
Danny R. Bottenus ◽  
Prashanta Dutta ◽  
Cornelius F. Ivory ◽  
...  
Keyword(s):  
Troponin I ◽  
Fluorescent Proteins ◽  
Biological Fluids ◽  
Microfluidic Channel ◽  
Fluorescein Isothiocyanate ◽  
Potential Drop ◽  
Cross Sectional Area ◽  
Sectional Area ◽  
Cross Sectional ◽  
Depth Direction

Concentration of bio-molecules prior to detection is very critical in the development of an integrated, multifunctional lab-on-a chip device for detection of ultra trace molecules from complex biological fluids such as serum, urine, or saliva. In this work, the preconcentration of a clinically relevant biomarker, cardiac troponin I (cTnI), is demonstrated in a cascade microfluidic channel using cationic isotachophoresis (ITP). The cascade chip is formed on PMMA (poly methyl methacrylate) with gradual changes in size both in width and depth direction to achieve a 100× reduction in overall cross sectional area between inlet (anode) and outlet (cathode) sections. The ITP experiments were conducted with two fluorescent proteins, FITC (Fluorescein isothiocyanate-conjugated) albumin and cTnI labeled with Pacific Blue. Potassium ions were used as the leader and hydronium ions were used as the terminator for these cationic ITP experiments. The microchip ITP demonstrates that it is possible to increase the concentration of cTnI by 10,000 folds using a potential drop of 400 V across a 3.5 cm long microchannel. The reduction in cross sectional area facilitates additional concentration gain, as the proteins migrate through cascade microchannel under discontinuous electric field and stacked into nearly pure zones.

Electrical Impedance Spectroscopy of the Human Prostate

2007 ◽  
Vol 54 (7) ◽  
pp. 1321-1327 ◽  
Author(s):  
R.J. Halter ◽  
A. Hartov ◽  
J.A. Heaney ◽  
K.D. Paulsen ◽  
A.R. Schned
Keyword(s):  
Impedance Spectroscopy ◽  
Electrical Impedance ◽  
Electrical Impedance Spectroscopy ◽  
Human Prostate

Pelvic Canal Narrowing Caused by Triple Pelvic Osteotomy in the Dog

1994 ◽  
Vol 07 (03) ◽  
pp. 110-113 ◽  
Author(s):  
D. L. Holmberg ◽  
M. B. Hurtig ◽  
H. R. Sukhiani
Keyword(s):  
Postoperative Complications ◽  
Pelvic Osteotomy ◽  
Cross Sectional Area ◽  
Sectional Area ◽  
Cross Sectional ◽  
Canal Width ◽  
Operative Complications ◽  
Post Operative Complications

SummaryDuring a triple pelvic osteotomy, rotation of the free acetabular segment causes the pubic remnant on the acetabulum to rotate into the pelvic canal. The resulting narrowing may cause complications by impingement on the organs within the pelvic canal. Triple pelvic osteotomies were performed on ten cadaver pelves with pubic remnants equal to 0, 25, and 50% of the hemi-pubic length and angles of acetabular rotation of 20, 30, and 40 degrees. All combinations of pubic remnant lengths and angles of acetabular rotation caused a significant reduction in pelvic canal-width and cross-sectional area, when compared to the inact pelvis. Zero, 25, and 50% pubic remnants result in 15, 35, and 50% reductions in pelvic canal width respectively. Overrotation of the acetabulum should be avoided and the pubic remnant on the acetabular segment should be minimized to reduce postoperative complications due to pelvic canal narrowing.When performing triple pelvic osteotomies, the length of the pubic remnant on the acetabular segment and the angle of acetabular rotation both significantly narrow the pelvic canal. To reduce post-operative complications, due to narrowing of the pelvic canal, overrotation of the acetabulum should be avoided and the length of the pubic remnant should be minimized.

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