Microbial community structure of anaerobic sludge for hydrogen production under different acid pretreatment conditions

2013 ◽  
Vol 5 (2) ◽  
pp. 023126 ◽  
Author(s):  
Jingjing Wan ◽  
Yanyan Ning ◽  
Xianyang Shi ◽  
Dawei Jin ◽  
Shouqin Li ◽  
...  
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Hydrogen Production ◽  
Microbial Community Structure ◽  
Anaerobic Sludge ◽  
Acid Pretreatment ◽  
Pretreatment Conditions

Microbial community structure of ethanol type fermentation in bio-hydrogen production

2007 ◽  
Vol 9 (5) ◽  
pp. 1112-1125 ◽  
Author(s):  
Nanqi Ren ◽  
Defeng Xing ◽  
Bruce E. Rittmann ◽  
Lihua Zhao ◽  
Tianhui Xie ◽  
...  
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Hydrogen Production ◽  
Microbial Community Structure

Impact of aluminum chloride on process performance and microbial community structure of granular sludge in an upflow anaerobic sludge blanket reactor for natural rubber processing wastewater treatment

2016 ◽  
Vol 74 (2) ◽  
pp. 500-507 ◽  
Author(s):  
Nguyen Thi Thanh ◽  
Takahiro Watari ◽  
Tran Phuong Thao ◽  
Masashi Hatamoto ◽  
Daisuke Tanikawa ◽  
...  
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Natural Rubber ◽  
Microbial Community Structure ◽  
Aluminum Chloride ◽  
Granular Sludge ◽  
Upflow Anaerobic Sludge Blanket ◽  
Uasb Reactor ◽  
Anaerobic Sludge ◽  
Anaerobic Sludge Blanket

In this study, granular sludge formation was carried out using an aluminum chloride supplement in an upflow anaerobic sludge blanket (UASB) reactor treating natural rubber processing wastewater. Results show that during the first 75 days after the start-up of the UASB reactor with an organic loading rate (OLR) of 2.65 kg-COD·m−3·day−1, it performed stably with a removal of 90% of the total chemical oxygen demand (COD) and sludge still remained in small dispersed flocs. However, after aluminum chloride was added at a concentration of 300 mg·L−1 and the OLR range was increased up to 5.32 kg-COD·m−3·day−1, the total COD removal efficiency rose to 96.5 ± 2.6%, with a methane recovery rate of 84.9 ± 13.4%, and the flocs began to form granules. Massively parallel 16S rRNA gene sequencing of the sludge retained in the UASB reactor showed that total sequence reads of Methanosaeta sp. and Methanosarcina sp., reported to be the key organisms for granulation, increased after 311 days of operation. This indicates that the microbial community structure of the retained sludge in the UASB reactor at the end of the experiment gave a good account of itself in not only COD removal, but also granule formation.

Microbial community structure and occurrence of diverse autotrophic ammonium oxidizing microorganisms in the anammox process

2010 ◽  
Vol 61 (11) ◽  
pp. 2723-2732 ◽  
Author(s):  
H. Bae ◽  
Y.-C. Chung ◽  
J.-Y. Jung
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Microbial Community Structure ◽  
Fragment Length Polymorphism ◽  
Amoa Gene ◽  
Continuous Operation ◽  
Anaerobic Sludge ◽  
Anammox Bacteria ◽  
Ammonia Oxidizing Bacteria ◽  
Anammox Process

The enrichment of anaerobic ammonium oxidizing (anammox) bacteria using an upflow anaerobic sludge bioreactor was successfully conducted for 400 days of continuous operation. The bacterial community structure of anammox bioreactor included Proteobacteria (42%), Chloroflexi (22%), Planctomycetes (20%), Chlorobi (7%), Bacteroidetes (5%), Acidobacteria (2%), and Actinobacteria (2%). All clones of Planctomycetes were affiliated with the anammox bacteria, Planctomycete KSU-1 (AB057453). The presence and diversity of ammonia oxidizing bacteria (AOB) and archaea (AOA) were identified by terminal restriction fragment length polymorphism (T-RFLP) based on the amoA gene sequences. The AOB in anammox bioreactor were affiliated with the Nitrosomonas europaea cluster. The T-RFLP result of AOA showed the diverse microbial community structure of AOA with three terminal restriction fragments (T-RFs).

scholarly journals Comparative Analysis of Bacterial and Archaeal Community Structure in Microwave Pretreated Thermophilic and Mesophilic Anaerobic Digesters Utilizing Mixed Sludge under Organic Overloading

Water ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 887 ◽  
Author(s):  
Gokce Kor-Bicakci ◽  
Emine Ubay-Cokgor ◽  
Cigdem Eskicioglu
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Microbial Community Structure ◽  
High Throughput Sequencing ◽  
Biogas Production ◽  
Archaeal Community ◽  
Rrna Gene ◽  
Anaerobic Digesters ◽  
Pretreatment Conditions ◽  
The Impact

The effects of microwave (MW) pretreatment were investigated by six anaerobic digesters operated under thermophilic and mesophilic conditions at high organic loading rates (4.9–5.7 g volatile solids/L/d). The experiments and analyses were mainly designed to reveal the impact of MW pretreatment and digester temperatures on the process stability and microbial community structure by correlating the composition of microbial populations with volatile fatty acid (VFA) concentrations. A slight shift from biogas production (with a reasonable methane content) to VFA accumulation was observed in the thermophilic digesters, especially in the MW-irradiated reactors. Microbial population structure was assessed using a high-throughput sequencing of 16S rRNA gene on the MiSeq platform. Microbial community structure was slightly affected by different MW pretreatment conditions, while substantially affected by the digester temperature. The phylum Bacteroidetes proliferated in the MW-irradiated mesophilic digesters by resisting high-temperature MW (at 160 °C). Hydrogenotrophic methanogenesis (mostly the genus of Methanothermobacter) was found to be a key route of methane production in the thermophilic digesters, whereas aceticlastic methanogenesis (mostly the genus of Methanosaeta) was the main pathway in the mesophilic digesters.

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