Translational diffusion of a transient radical studied by the transient grating method, pyrazinyl radical in 2‐propanol

1993 ◽  
Vol 98 (8) ◽  
pp. 6257-6262 ◽  
Author(s):  
Masahide Terazima ◽  
Noboru Hirota
Keyword(s):  
Translational Diffusion ◽  
Transient Grating

Translational diffusion of transient radicals studied by the transient grating method

1995 ◽  
Vol 65-66 ◽  
pp. 401-404 ◽  
Author(s):  
Masahide Terazima ◽  
Koichi Okamoto ◽  
Noboru Hirota
Keyword(s):  
Translational Diffusion ◽  
Transient Grating

Heterogeneity of Size and Distribution of Intramembrane Particles in the Plasma Membrane of Yeast

1977 ◽  
Vol 35 ◽  
pp. 596-597
Author(s):  
E. Keyhani
Keyword(s):  
Plasma Membrane ◽  
Candida Utilis ◽  
Synthetic Medium ◽  
Freeze Fracture ◽  
Translational Diffusion ◽  
Yeast Cells ◽  
Distilled Water ◽  
Intramembrane Particles ◽  
The Matrix ◽  
Water Cell

The matrix of biological membranes consists of a lipid bilayer into which proteins or protein aggregates are intercalated. Freeze-fracture techni- ques permit these proteins, perhaps in association with lipids, to be visualized in the hydrophobic regions of the membrane. Thus, numerous intramembrane particles (IMP) have been found on the fracture faces of membranes from a wide variety of cells (1-3). A recognized property of IMP is their tendency to form aggregates in response to changes in experi- mental conditions (4,5), perhaps as a result of translational diffusion through the viscous plane of the membrane. The purpose of this communica- tion is to describe the distribution and size of IMP in the plasma membrane of yeast (Candida utilis).Yeast cells (ATCC 8205) were grown in synthetic medium (6), and then harvested after 16 hours of culture, and washed twice in distilled water. Cell pellets were suspended in growth medium supplemented with 30% glycerol and incubated for 30 minutes at 0°C, centrifuged, and prepared for freeze-fracture, as described earlier (2,3).

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