Observation and quantitation of light emission from cytochrome c using Soret band laser excitation

1981 ◽  
Vol 75 (1) ◽  
pp. 490-491 ◽  
Author(s):  
P. M. Champion ◽  
G. J. Perreault
Keyword(s):  
Cytochrome C ◽  
Laser Excitation ◽  
Light Emission ◽  
Soret Band

Resonance Raman investigations of chloroperoxidase, horseradish peroxidase, and cytochrome c using Soret band laser excitation

Biochemistry ◽  
1979 ◽  
Vol 18 (11) ◽  
pp. 2280-2290 ◽  
Author(s):  
Ronald D. Remba ◽  
Paul M. Champion ◽  
Douglas B. Fitchen ◽  
Robert Chiang ◽  
Lowell P. Hager
Keyword(s):  
Cytochrome C ◽  
Horseradish Peroxidase ◽  
Laser Excitation ◽  
Resonance Raman ◽  
Soret Band

scholarly journals Low-level chemiluminescence of hydroperoxide-supplemented cytochrome c

1980 ◽  
Vol 187 (1) ◽  
pp. 131-140 ◽  
Author(s):  
Enrique Cadenas ◽  
Alberto Boveris ◽  
Britton Chance
Keyword(s):  
Cytochrome C ◽  
Singlet Oxygen ◽  
Light Emission ◽  
Cumene Hydroperoxide ◽  
Present System ◽  
Butyl Hydroperoxide ◽  
Low Level ◽  
Radical Trap ◽  
Organic Hydroperoxides ◽  
Ferricytochrome C

Ferricytochrome c showed low-level chemiluminescence, with a light-emission measured of about 1×103–3×103 counts/s, when supplemented with organic hydroperoxides. Tertiary hydroperoxides (cumene hydroperoxide and t-butyl hydroperoxide) showed a saturation behaviour at about 5mm-hydroperoxide, whereas primary hydroperoxides showed a quadratic dependence on the hydroperoxide concentration. Chemiluminescence depended linearly on cytochrome c concentration, and optimal light-emission was observed at [t-butyl hydroperoxide]/[ferricytochrome c] ratios of 160–500. Hydroperoxide-supplemented ferricytochrome c consumed O2 at a rate of 1.0μmol/min per μmol of cytochrome c; the rate of O2 uptake was linearly related to the concentration of cytochrome c. The Soret absorption band of ferricytochrome c decreased about 64% after incubation with t-butyl hydroperoxide, whereas the 530nm band was almost totally abolished. Light-emission was (a) inhibited competitively by cyanide. (b) inhibited by singlet-oxygen quenchers (e.g. β-carotene), scavengers (e.g. dimethylfuran) and traps (e.g. histidine and tryptophan) and (c) increased by singlet-oxygen-chemiluminescence enhancer 1,4-diazabicyclo[2.2.2]-octane. Superoxide dismutase had no effect on the present system. The participation of free radicals is suggested by the effect of the radical trap 2,5-di-t-butylquinol. Singlet-oxygen dimol emission seems to be mainly responsible for the observed light-emission; a mechanism that can account for the major part of the present experimental observations is proposed.

Mechanism for Size Dependence of Light Emission From ZnO Nanocrystallites

2007 ◽  
Author(s):  
Fuli Zhao ◽  
Xiaofang Wang ◽  
Pingbo Xie ◽  
Ningsheng Xu ◽  
Zhizhan Xu
Keyword(s):  
Band Gap ◽  
Zno Nanoparticles ◽  
Size Dependence ◽  
Laser Excitation ◽  
Light Emission ◽  
Surface States ◽  
Picosecond Laser ◽  
One Dimensional ◽  
1D Model ◽  
Critical Grain Size

Broadband light emission of different sized ZnO nanoparticles (17 nm to 300 nm) has been measured under picosecond laser excitation. Each spectrum consists of three Gaussian components including one emerging inside band-gap. Such inside band-gap band shows a clear dependence of the weighted intensity on the sizes of nanoparticles. Originated from our simplified one-dimensional (1D) model it has been qualitatively deduced that such size dependence match with the surface-state modified band structure. Additionally, a critical grain size as 110 nm for surface states recombination of ZnO nanoparticles has been found experimentally.

A Polar Liquid Zwitterion Does Not Critically Destroy Cytochrome c at High Concentration: An Initial Comparative Study with a Polar Ionic Liquid

2019 ◽  
Vol 72 (2) ◽  
pp. 139 ◽  
Author(s):  
Kosuke Kuroda ◽  
Chiaki Kodo ◽  
Kazuaki Ninomiya ◽  
Kenji Takahashi
Keyword(s):  
Ionic Liquid ◽  
Cytochrome C ◽  
Soret Band ◽  
Liquid Solution ◽  
Acetate Solution ◽  
High Concentration ◽  
Liquid Solutions ◽  
High Concentration Range ◽  
Ionic Liquid Solution ◽  
Better Than

A polar carboxylate-type zwitterion with a small volume of water can dissolve cytochrome c without significant disruption, compared with the case of a popular polar carboxylate-type ionic liquid, 1-ethyl-3-methylimidazolium acetate. A change in the Soret, Q, and 615nm bands was not observed in the 80 wt-% polar zwitterion solution, whereas a shift in the Soret band, diminishing Q band, and appearance of the 615nm band was found in the 80 wt-% polar ionic liquid solution. It suggests that concentrated polar ionic liquid solutions critically disrupt the structure of cytochrome c, and the polar zwitterion solution used in this study was better than a 1-ethyl-3-methylimidazolium acetate solution in a high concentration range.

scholarly journals 1P-119 Soret band absorption spectra of cytochrome c oxidase in the crystalline state(The 46th Annual Meeting of the Biophysical Society of Japan)

2008 ◽  
Vol 48 (supplement) ◽  
pp. S39
Author(s):  
Masao MOCHIZUKI ◽  
Kazumasa MURAMOTO ◽  
Kyoko SHINZAWA-ITOH ◽  
Tomitake TSUKIHARA ◽  
Shinya YOSHIKAWA
Keyword(s):  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Annual Meeting ◽  
Absorption Spectra ◽  
Crystalline State ◽  
Soret Band ◽  
Biophysical Society ◽  
Band Absorption

scholarly journals Low-level chemiluminescence of bovine heart submitochondrial particles

1980 ◽  
Vol 186 (3) ◽  
pp. 659-667 ◽  
Author(s):  
Enrique Cadenas ◽  
Alberto Boveris ◽  
Britton Chance
Keyword(s):  
Lipid Peroxidation ◽  
Cytochrome C ◽  
Light Emission ◽  
Cumene Hydroperoxide ◽  
Radical Reactions ◽  
Submitochondrial Particles ◽  
Butyl Hydroperoxide ◽  
Low Level ◽  
Bovine Heart ◽  
Β Carotene

Submitochondrial particles from bovine heart mitochondria showed low-level chemiluminescence when supplemented with organic hydroperoxides. Chemiluminescence seems to measure integratively radical reactions involved in lipid peroxidation and related processes. Maximal light-emission was about 1500 counts/s and was reached 2–10min after addition of hydroperoxides. Ethyl hydroperoxide, cumene hydroperoxide and t-butyl hydroperoxide were effective in that order. Antimycin and rotenone increased chemiluminescence by 50–60%; addition of substrates, NADH and succinate did not produce marked changes in the observed chemiluminescence. Cyanide inhibited chemiluminescence; half-maximal inhibitory effect was obtained with 0.03mm-cyanide and the inhibition was competitive with respect to t-butyl hydroperoxide. Externally added cytochrome c (10–20μm) had a marked stimulatory effect on chemiluminescence, namely a 12-fold increase in light-emission of antimycin-inhibited submitochondrial particles. Stimulation of hydroperoxide-induced chemiluminescence of submitochondrial particles by cytochrome c was matched by a burst of O2 consumption. O2 is believed to participate in the chain radical reactions that lead to lipid peroxidation. Superoxide anion seems to be involved in the chemiluminescence reactions as long as light-emission was 50–60% inhibitible by superoxide dismutase. Singlet-oxygen quenchers, e.g. β-carotene and 1,4-diazabicyclo[2,2,2]-octane, affected light-emission. β-Carotene was effective either when incorporated into the membranes or added to the cuvette. The present paper suggests that singlet molecular oxygen is mainly responsible for the light-emission in the hydroperoxide-supplemented submitochondrial particles.

Soret Band of the Gas-Phase Ferri-Cytochrome c

2012 ◽  
Vol 3 (6) ◽  
pp. 698-702 ◽  
Author(s):  
Claire Brunet ◽  
Rodolphe Antoine ◽  
Jérôme Lemoine ◽  
Philippe Dugourd
Keyword(s):  
Cytochrome C ◽  
Gas Phase ◽  
Soret Band

scholarly journals Chemiluminescence of lipid vesicles supplemented with cytochrome c and hydroperoxide

1980 ◽  
Vol 188 (3) ◽  
pp. 577-583 ◽  
Author(s):  
E Cadenas ◽  
A Boveris ◽  
B Chance
Keyword(s):  
Cytochrome C ◽  
Light Emission ◽  
Lipid Vesicles ◽  
Soya Bean ◽  
Phase Iii ◽  
Lipid Vesicle ◽  
Present System ◽  
Dipalmitoyl Phosphatidylcholine ◽  
Radical Trap ◽  
Enhanced Chemiluminescence

The increase in light emission of hydroperoxide-supplemented cytochrome c observed on addition of lipid vesicles was related to the degree of unsaturation of the fatty acids of the phospholipids: dipalmitoyl phosphatidylcholine was without effect, whereas dioleoyl phosphatidylcholine and soya-bean phosphatidylcholine enhanced chemiluminescence 2- and 3-fold respectively. Effects on light-emission were similar to those on O2 uptake. The chemiluminescence of the present system was sensitive to cyanide and to the radical trap 2,5-di-t-butylquinol, indicating a catlytic activity of cytochrome c and the presence of free-radical species respectively. Lipid-vesicle enhanced chemiluminescence showed different kinetic behaviours, apparently depending on unsaturation: three phases are described for soya-bean phosphatidylcholine, whereas only one phase was present in mixtures containing dipalmitoyl and dioleoyl phospholipids. Chemiluminescence of lipid vesicles supplemented with cytochrome c and hydroperoxide showed similar kinetic patterns with H2O2 and primary (ethyl) and tertiary (t-butyl and cumene) hydroperoxides. Participation of singlet molecular oxygen, mainly on the phase III of chemiluminescence, is suggested by the increase of light-emission by 1,4-diazabicyclo[2.2.2]-octane as well as by data from spectral analysis.

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