Application of intensity correlation spectroscopy to the measurement of continuous distributions of spherical particles

1977 ◽  
Vol 67 (7) ◽  
pp. 3151-3156 ◽  
Author(s):  
R. L. McCally ◽  
C. B. Bargeron
Keyword(s):  
Correlation Spectroscopy ◽  
Spherical Particles ◽  
Continuous Distributions ◽  
Intensity Correlation

Intensity-Correlation Spectroscopy

1971 ◽  
Vol 4 (5) ◽  
pp. 2033-2050 ◽  
Author(s):  
V. Degiorgio ◽  
J. B. Lastovka
Keyword(s):  
Correlation Spectroscopy ◽  
Intensity Correlation

Monte Carlo simulation of fluorescence correlation spectroscopy data

2011 ◽  
Vol 76 (3) ◽  
pp. 207-222 ◽  
Author(s):  
Peter Košovan ◽  
Filip Uhlík ◽  
Jitka Kuldová ◽  
Miroslav Štěpánek ◽  
Zuzana Limpouchová ◽  
...  
Keyword(s):  
Monte Carlo Simulation ◽  
Monte Carlo ◽  
Diffusion Coefficients ◽  
Fluorescence Correlation Spectroscopy ◽  
Rotational Diffusion ◽  
Correlation Spectroscopy ◽  
Analytical Models ◽  
Spherical Particles ◽  
Fluorescence Signal ◽  
Fluorescence Correlation

We employed the Monte Carlo simulation methodology to emulate the diffusion of fluorescently labeled particles and understand the source of differences between values of diffusion coefficients (and consequently hydrodynamic radii) of fluorescently labeled nanoparticles measured by fluorescence correlation spectroscopy (FCS) and dynamic light scattering (DLS). We used the simulation program developed in our laboratory and studied the diffusion of spherical particles of different sizes, which are labeled on their surface. In this study, we focused on two complicating effects: (i) multiple labeling and (ii) rotational diffusion which affect the fluorescence signal from large particles and hinder the analysis of autocorrelation functions according to simple analytical models. We have shown that the fluorescence fluctuations can be well fitted using the analytical model for small point-like particles, but the obtained parameters deviate in some cases significantly from the real ones. It means that the current data treatment yields apparent values of diffusion coefficients and other parameters only and the interpretation of experimental results for systems of particles with sizes comparable to the size of the active illuminated volume requires great care and precaution.

Fluorescence correlation spectroscopy applied to rotational diffusion of macromolecules

1976 ◽  
Vol 9 (1) ◽  
pp. 69-81 ◽  
Author(s):  
Måns Ehrenberg ◽  
Rudolf Rigler
Keyword(s):  
Rotational Motion ◽  
Fluorescence Correlation Spectroscopy ◽  
Rotational Diffusion ◽  
Quantitative Relationship ◽  
Correlation Spectroscopy ◽  
Spherical Particles ◽  
Fluorescence Correlation ◽  
Fluorescence Light

A quantitative relationship between polarization properties of fluorescence light and molecular rotational diffusion was first derived by Perrin (1926). His results, which concerned spherical particles, have later been refined to the more complex rotational motion of asymmetric bodies (Memming, 1961; Chuang & Eisenthal, 1972; Ehrenberg & Rigler, 1972; Belford, Belford & Weber, 1972).

scholarly journals Intensity-Correlation Spectroscopy

1966 ◽  
Vol 142 (1) ◽  
pp. 25-32 ◽  
Author(s):  
M. L. Goldberger ◽  
H. W. Lewis ◽  
K. M. Watson
Keyword(s):  
Correlation Spectroscopy ◽  
Intensity Correlation
Sign in / Sign up

Export Citation Format

Share Document