scholarly journals Embryonic stem cell bioprinting for uniform and controlled size embryoid body formation

2011 ◽  
Vol 5 (2) ◽  
pp. 022207 ◽  
Author(s):  
Feng Xu ◽  
BanuPriya Sridharan ◽  
ShuQi Wang ◽  
Umut Atakan Gurkan ◽  
Brian Syverud ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Embryoid Body ◽  
Embryonic Stem ◽  
Body Formation ◽  
Embryoid Body Formation

Efficiency of embryoid body formation and hematopoietic development from embryonic stem cells in different culture systems

2002 ◽  
Vol 78 (4) ◽  
pp. 442-453 ◽  
Author(s):  
Stephen M. Dang ◽  
Michael Kyba ◽  
Rita Perlingeiro ◽  
George Q. Daley ◽  
Peter W. Zandstra
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryoid Body ◽  
Embryonic Stem ◽  
Body Formation ◽  
Hematopoietic Development ◽  
Culture Systems ◽  
Embryoid Body Formation

scholarly journals Controlling Embryonic Stem Cell Growth and Differentiation by Automation

2012 ◽  
Vol 17 (9) ◽  
pp. 1171-1179 ◽  
Author(s):  
Michael P. Kowalski ◽  
Amy Yoder ◽  
Li Liu ◽  
Laura Pajak
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Drug Discovery ◽  
Embryonic Stem Cells ◽  
Embryonic Stem Cell ◽  
High Throughput ◽  
Embryoid Body ◽  
Embryonic Stem ◽  
Basic Research ◽  
Complex Biological Process

Despite significant use in basic research, embryonic stem cells have just begun to be used in the drug discovery process. Barriers to the adoption of embryonic stem cells in drug discovery include the difficulty in growing cells and inconsistent differentiation to the desired cellular phenotype. Embryonic stem cell cultures require consistent and frequent handling to maintain the cells in a pluripotent state. In addition, the preferred hanging drop method of embryoid body (EB) differentiation is not amenable to high-throughput methods, and suspension cultures of EBs show a high degree of variability. Murine embryonic stem cells passaged on an automated platform maintained ≥90% viability and pluripotency. We also developed a method of EB formation using 384-well microplates that form a single EB per well, with excellent uniformity across EBs. This format facilitated high-throughput differentiation and enabled screens to optimize directed differentiation into a desired cell type. Using this approach, we identified conditions that enhanced cardiomyocyte differentiation sevenfold. This optimized differentiation method showed excellent consistency for such a complex biological process. This automated approach to embryonic stem cell handling and differentiation can provide the high and consistent yields of differentiated cell types required for basic research, compound screens, and toxicity studies.

scholarly journals Hypoxic Culture Maintains Self-Renewal and Enhances Embryoid Body Formation of Human Embryonic Stem Cells

2010 ◽  
Vol 16 (9) ◽  
pp. 2901-2913 ◽  
Author(s):  
Hsin-Fu Chen ◽  
Hung-Chih Kuo ◽  
Shau-Ping Lin ◽  
Chung-Liang Chien ◽  
Ming-Shan Chiang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryoid Body ◽  
Embryonic Stem ◽  
Self Renewal ◽  
Body Formation ◽  
Embryoid Body Formation

scholarly journals Puerarin Enhances Ca2+ Reuptake and Ca2+ Content of Sarcoplasmic Reticulum in Murine Embryonic Stem Cell-Derived Cardiomyocytes via Upregulation of SERCA2a

2017 ◽  
Vol 44 (3) ◽  
pp. 1199-1212
Author(s):  
Lu Wang ◽  
Yurong Cui ◽  
Qinghua Liu ◽  
Yuanlong Song ◽  
Qinghua Hu ◽  
...  
Keyword(s):  
Stem Cell ◽  
Sarcoplasmic Reticulum ◽  
Protein Expression ◽  
Embryonic Stem Cell ◽  
Embryoid Body ◽  
Embryonic Stem ◽  
Calcium Transient ◽  
Cardiac Differentiation ◽  
Patch Clamps

Background/Aims: The embryonic stem cell-derived cardiomyocytes (ES-CMs) serve as potential sources for cardiac regenerative therapy. However, the immature sarcoplasmic reticulum (SR) function of ES-CMs prevents its application. In this report, we examined the effect of puerarin, an isoflavone compound, on SR function of murine ES-CMs. Methods: Murine ES-CMs were harvested by embryoid body-based differentiation method. Confocal calcium imaging and whole-cell patch clamps were performed to assess the function of SR. The mRNA expression levels of SR-related genes were examined by quantitative PCR. The protein expression of sarcoplasmic reticulum calcium-ATPase 2a (SERCA2a) was evaluated by immunofluorescent and western blot. Results: Long-term application of puerarin promotes basic properties of spontaneous calcium transient with increased amplitude, decay velocity, and decreased duration. Puerarin fails to alter ICa,L but increases the Ca2+ content of SR. Puerarin-treated ES-CMs have intact SR Ca2+ cycling with more SR Ca2+ reuptake. Long-term application of puerarin asynchronously upregulates the mRNA and protein expression of SERCA2a, as well as the transcripts of calsequestrin and triadin in developing ES-CMs. Application of puerarin during the stage of post-cardiac differentiation upregulates dose-dependently the transcripts of SERCA2a, phospholamban and tridin which can be reversed by the inhibitors of the PI3K/Akt and MAPK/ERK signaling pathways, but shows no effect on the protein expression of SERCA2a. Conclusion: This study demonstrates that long-term puerarin treatment enhances Ca2+ reuptake and Ca2+ content via upregulation of SERCA2a.

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