scholarly journals Highly-efficient single-cell capture in microfluidic array chips using differential hydrodynamic guiding structures

2011 ◽  
Vol 98 (12) ◽  
pp. 123701 ◽  
Author(s):  
Jaehoon Chung ◽  
Young-Ji Kim ◽  
Euisik Yoon
Keyword(s):  
Single Cell ◽  
Cell Capture ◽  
Highly Efficient

scholarly journals Efficient analysis of a small number of cancer cells at the single-cell level using an electroactive double-well array

Lab on a Chip ◽  
2016 ◽  
Vol 16 (13) ◽  
pp. 2440-2449 ◽  
Author(s):  
Soo Hyeon Kim ◽  
Teruo Fujii
Keyword(s):  
Single Cell ◽  
Cancer Cells ◽  
Single Cells ◽  
Capture Efficiency ◽  
Single Cell Level ◽  
Cell Capture ◽  
Cell Level ◽  
Cell Trapping ◽  
Highly Efficient ◽  
Single Cell Trapping

The electroactive double well-array consists of trap-wells for highly efficient single-cell trapping using dielectrophoresis (cell capture efficiency of 96 ± 3%) and reaction-wells that confine cell lysates for analysis of intracellular materials from single cells.

Single cell capture, isolation, and long‐term in‐situ imaging using quantitative self‐interference spectroscopy

2021 ◽  
Author(s):  
Rongxin Fu ◽  
Ya Su ◽  
Ruliang Wang ◽  
Xue Lin ◽  
Xiangyu Jin ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Capture ◽  
In Situ Imaging

scholarly journals A simple microdevice for single cell capture, array, release, and fast staining using oscillatory method

2018 ◽  
Vol 12 (3) ◽  
pp. 034105 ◽  
Author(s):  
Dantong Cheng ◽  
Yang Yu ◽  
Chao Han ◽  
Mengjia Cao ◽  
Guang Yang ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Capture

scholarly journals Integration of marker-free selection of single cells at a wireless electrode array with parallel fluidic isolation and electrical lysis

2019 ◽  
Vol 10 (5) ◽  
pp. 1506-1513 ◽  
Author(s):  
Min Li ◽  
Robbyn K. Anand
Keyword(s):  
Single Cell ◽  
Single Cells ◽  
Electrode Array ◽  
Cell Capture ◽  
Bipolar Electrodes ◽  
Manual Intervention ◽  
Electrical Lysis ◽  
Marker Free ◽  
Selection Of

We present integration of selective single-cell capture at an array of wireless electrodes (bipolar electrodes, BPEs) with transfer into chambers, reagent exchange, fluidic isolation and rapid electrical lysis in a single platform, thus minimizing sample loss and manual intervention steps.

Abstract 2291: Single cell capture and molecular analysis of live CTCs using integrated microwells and single cell aspirator

2019 ◽  
Author(s):  
Charlotte N. Stahlfeld ◽  
Jake J. Tokar ◽  
David Quigley ◽  
David Niles ◽  
Jamie M. Sperger ◽  
...  
Keyword(s):  
Single Cell ◽  
Molecular Analysis ◽  
Cell Capture

Highly efficient acoustophoretic single cell-supernatant separation inside nanoliter droplets

2020 ◽  
Vol 148 (4) ◽  
pp. 2785-2785
Author(s):  
Michael Gerlt ◽  
Dominik Haidas ◽  
Alexandre Ratschat ◽  
Philipp Suter ◽  
Petra Dittrich ◽  
...  
Keyword(s):  
Single Cell ◽  
Highly Efficient ◽  
Cell Supernatant

A highly efficient introduction system for single cell- ICP-MS and its application to detection of copper in single human red blood cells

Talanta ◽  
2020 ◽  
Vol 206 ◽  
pp. 120174 ◽  
Author(s):  
Yupin Cao ◽  
Jinsu Feng ◽  
Lifu Tang ◽  
Chunhe Yu ◽  
Guichun Mo ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Single Cell ◽  
Blood Cells ◽  
Highly Efficient ◽  
Human Red Blood Cells ◽  
Icp Ms

Capillary Force Driven Single-Cell Spiking Apparatus for Studying Circulating Tumor Cells

2018 ◽  
Author(s):  
Jacob Amontree ◽  
Kangfu Chen ◽  
Jose Varillas ◽  
Z. Hugh Fan
Keyword(s):  
Single Cell ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Single Cells ◽  
Lymphoblastic Leukemia ◽  
Surface Membrane ◽  
Critical Element ◽  
Cell Capture ◽  
Biomedical Sciences ◽  
Low Concentrations

The characterization of single cells within heterogeneous populations has great impact on both biomedical sciences and cancer research. By investigating cellular compositions on a broad scale, pertinent outliers may be lost in the sample set. Alternatively, an investigation focused on the behavior of specific cells, such as circulating tumor cells (CTCs), will reveal genetic biomarkers or phenotypic characteristics associated with cancer and metastasis. On average, CTC concentration in peripheral blood is extremely low, as few as one to two per billion of healthy blood cells. Consequently, the critical element lacking in many methods of CTC detection is accurate cell capture efficiency at low concentrations. To simulate CTC isolation, researchers usually spike small amounts of tumor cells to healthy blood for separation. However, spiking tumor cells at extremely low concentrations is challenging in a standard laboratory setting. We report our study on an innovative apparatus and method designed for low-cost, precise, and replicable single-cell spiking (SCS). Our SCS method operates solely from capillary aspiration without the reliance on external laboratory equipment. To ensure that our method does not affect the viability of each cell, we investigated the effects of surface membrane tensions induced by aspiration. Finally, we performed affinity-based CTC isolation using human acute lymphoblastic leukemia cells (CCRF-CEM) spiked into healthy whole blood with the SCS technique. The results of the isolation experiments demonstrate the reliability of our method in generating low-concentration cell samples.

Highly efficient single-cell analysis of microbial cells by time-resolved inductively coupled plasma mass spectrometry

2014 ◽  
Vol 29 (9) ◽  
pp. 1598-1606 ◽  
Author(s):  
Shin-ichi Miyashita ◽  
Alexander S. Groombridge ◽  
Shin-ichiro Fujii ◽  
Ayumi Minoda ◽  
Akiko Takatsu ◽  
...  
Keyword(s):  
Single Cell ◽  
Inductively Coupled Plasma ◽  
Single Cell Analysis ◽  
High Time Resolution ◽  
Time Resolved ◽  
Microbial Cells ◽  
Inductively Coupled ◽  
Highly Efficient ◽  
Icp Ms ◽  
Plasma Mass Spectrometry

Highly efficient single-cell elemental analysis of microbial cells was achieved using a developed ICP-MS system with approximately 100% cell introduction efficiency and high time resolution.

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