Monitoring Biomimetic Lipid Membrane Compositions And Structural Changes In Bound Protein On Biocompatible Gold Nanoparticles Surfaces By Vibrational Spectroscopy

2010 ◽  
Author(s):  
Tibebe Lemma ◽  
Robert J. Forster ◽  
Tia E. Keyes ◽  
P. M. Champion ◽  
L. D. Ziegler
Keyword(s):  
Gold Nanoparticles ◽  
Vibrational Spectroscopy ◽  
Structural Changes ◽  
Lipid Membrane

scholarly journals Local Enhancement of Lipid Membrane Permeability Induced by Irradiated Gold Nanoparticles

ACS Nano ◽  
2017 ◽  
Vol 11 (12) ◽  
pp. 12553-12561 ◽  
Author(s):  
Andrea Torchi ◽  
Federica Simonelli ◽  
Riccardo Ferrando ◽  
Giulia Rossi
Keyword(s):  
Gold Nanoparticles ◽  
Membrane Permeability ◽  
Lipid Membrane ◽  
Local Enhancement

scholarly journals The Structural Integrity of the Model Lipid Membrane during Induced Lipid Peroxidation: The Role of Flavonols in the Inhibition of Lipid Peroxidation

Antioxidants ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 430 ◽  
Author(s):  
Anja Sadžak ◽  
Janez Mravljak ◽  
Nadica Maltar-Strmečki ◽  
Zoran Arsov ◽  
Goran Baranović ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Lipid Bilayers ◽  
Lipid Membranes ◽  
Structural Changes ◽  
Structural Integrity ◽  
Lipid Membrane ◽  
Membrane Properties ◽  
Structural Reorganization ◽  
Unsaturated Lipids ◽  
Oxidative Attack

The structural integrity, elasticity, and fluidity of lipid membranes are critical for cellular activities such as communication between cells, exocytosis, and endocytosis. Unsaturated lipids, the main components of biological membranes, are particularly susceptible to the oxidative attack of reactive oxygen species. The peroxidation of unsaturated lipids, in our case 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), induces the structural reorganization of the membrane. We have employed a multi-technique approach to analyze typical properties of lipid bilayers, i.e., roughness, thickness, elasticity, and fluidity. We compared the alteration of the membrane properties upon initiated lipid peroxidation and examined the ability of flavonols, namely quercetin (QUE), myricetin (MCE), and myricitrin (MCI) at different molar fractions, to inhibit this change. Using Mass Spectrometry (MS) and Fourier Transform Infrared Spectroscopy (FTIR), we identified various carbonyl products and examined the extent of the reaction. From Atomic Force Microscopy (AFM), Force Spectroscopy (FS), Small Angle X-Ray Scattering (SAXS), and Electron Paramagnetic Resonance (EPR) experiments, we concluded that the membranes with inserted flavonols exhibit resistance against the structural changes induced by the oxidative attack, which is a finding with multiple biological implications. Our approach reveals the interplay between the flavonol molecular structure and the crucial membrane properties under oxidative attack and provides insight into the pathophysiology of cellular oxidative injury.

scholarly journals Size matters: Size Dependency of Gold Nanoparticles Interacting with Model Membranes

2019 ◽  
Author(s):  
Claudia Contini ◽  
James W. Hindley ◽  
Tom Macdonald ◽  
Joseph Barritt ◽  
Oscar Ces ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Lipid Bilayers ◽  
Lipid Membrane ◽  
Rapid Development ◽  
Membrane System ◽  
Size Dependent ◽  
Research Fields ◽  
Large Unilamellar Vesicles ◽  
Wide Range ◽  
Experimental Characterisation

<p><b>The rapid development of nanomaterials has led to an increase in the number and variety of engineered nanomaterials (ENMs) in the environment. Gold nanoparticles (AuNPs) are an example of a commonly studied ENM whose highly tailorable properties have generated significant interest through a wide range of research fields. In the present work, we report the first qualitative as well as quantitative experimental characterisation of the AuNP-membrane interaction. We investigate the interactions between citrate-stabilised AuNPs (diameters 5, 10, 25, 35, 50, 60 nm) and large unilamellar vesicles (LUVs) acting as a model membrane system. LUVs were prepared in two different formulations using 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine (POPC) and 1,2-dileoyl-sn-glycero-3-phosphocholine (DOPC). Our results show that the interaction between AuNPs and LUVs is size dependent; in particular, we reveal the existence of two AuNP’s critical diameters which determine the fate of AuNPs in contact with a lipid membrane. The results provide a new understanding of the size dependent interaction between AuNPs and lipid bilayers of direct relevance to nanotoxicology and to the design of NP vectors.</b></p>

Surface-Enhanced Vibrational Spectroscopy of Adsorbates on Microemulsion Synthesized Gold Nanoparticles

2011 ◽  
Vol 65 (7) ◽  
pp. 741-745 ◽  
Author(s):  
Ayuba Fasasi ◽  
Peter R. Griffiths ◽  
Horng-Bin Pan ◽  
Chien M. Wai
Keyword(s):  
Gold Nanoparticles ◽  
Vibrational Spectroscopy ◽  
Surface Enhanced

Stability of carbon monoxide oxidation process on gold nanoparticles

2020 ◽  
Vol 8 (1) ◽  
pp. 116-124
Author(s):  
P. P. Kostrobij ◽  
◽  
I. A. Ryzha ◽  
Keyword(s):  
Carbon Monoxide ◽  
Gold Nanoparticles ◽  
Reaction Mechanisms ◽  
Structural Changes ◽  
Oxidation Process ◽  
Lyapunov Method ◽  
Catalyst Surface ◽  
Carbon Monoxide Oxidation ◽  
One Step ◽  
The Stability

The stability conditions for mathematical models of carbon monoxide oxidation on the surface of gold nanoparticles are investigated. The cases of reaction mechanisms of one-step and step-by-step transformation of reagents are consecutively considered. Using the stability analysis by Lyapunov method, it is shown that models which take into account the possibility of structural changes of the catalyst surface can predict the occurrence of oscillatory mode in the system as a result of Hopf instability.

scholarly journals Membrane association of VAMP2 SNARE motif in cells and its regulation by different lipid phases of synaptic vesicle membrane

2019 ◽  
Author(s):  
Chuchu Wang ◽  
Jia Tu ◽  
Shengnan Zhang ◽  
Bin Cai ◽  
Zhenying Liu ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Structural Changes ◽  
Lipid Membrane ◽  
Environmental Changes ◽  
Snare Complex ◽  
Membrane Association ◽  
Vesicle Membrane ◽  
Spatial Regulation ◽  
Snare Motif ◽  
Mechanistic Basis

SummaryVesicle associated membrane protein 2 (VAMP2) contains a conserved SNARE motif that forms helix bundles with the homologous motifs of syntaxin-1 and SNAP25 to assemble into a SNARE complex for the exocytosis of synaptic vesicles (SV). Prior to SNARE assembly, the structure of VAMP2 is unclear. Here, using in-cell NMR spectroscopy, we described the dynamic membrane association of VAMP2 SNARE motif in mammalian cells at atomic resolution, and further tracked the intracellular structural changes of VAMP2 upon the lipid environmental changes. The underlying mechanistic basis was then investigated by solution NMR combined with mass-spectrometry-based lipidomic profiling. We analyzed the lipid compositions of lipid-raft and non-raft phases of SV membrane and revealed that VAMP2 configures distinctive conformations in different phases of SV membrane. The phase of cholesterol-rich lipid rafts could largely weaken the association of SNARE motif with SV membrane and thus, facilitate vesicle docking; While in the non-raft phase, the SNARE motif tends to hibernate on SV membrane with minor activity. Our work provides a spatial regulation of different lipid membrane phases to the structure of core SNARE proteins, which deepens our knowledge on the modulation of SNARE machinery.

scholarly journals How gold nanoparticles can be used to probe the structural changes of a pH-responsive hydrogel

2017 ◽  
Vol 19 (7) ◽  
pp. 5102-5112 ◽  
Author(s):  
Maximilian M. Schmidt ◽  
Shanglin Wu ◽  
Zhengxing Cui ◽  
Nam T. Nguyen ◽  
Michael Faulkner ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Surface Plasmon Resonance ◽  
Fluorescence Quenching ◽  
Surface Plasmon ◽  
Network Structure ◽  
Structural Changes ◽  
Local Network ◽  
Ph Responsive ◽  
Resonance Signal ◽  
Surface Plasmon Resonance Signal

Incorporating gold nanoparticles within a pH-responsive hydrogel enables the surface plasmon resonance signal and metal-induced fluorescence quenching to be used to study local network structure and probe gel swelling remotely.

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