scholarly journals Scattering analysis of two-dimensional electrons in AlGaN/GaN with bulk related parameters extracted by simple parallel conduction extraction method

2010 ◽  
Vol 108 (1) ◽  
pp. 013712 ◽  
Author(s):  
S. B. Lisesivdin ◽  
A. Yildiz ◽  
N. Balkan ◽  
M. Kasap ◽  
S. Ozcelik ◽  
...  
Keyword(s):  
Extraction Method ◽  
Two Dimensional ◽  
Parallel Conduction

scholarly journals Protein extraction method of Metroxylon sagu leaf for high resolution two dimensional gel electrophoresis and comparative proteomics

2019 ◽  
Author(s):  
Mehvish Nisar ◽  
Hasnain Hussain
Keyword(s):  
Gel Electrophoresis ◽  
Extraction Method ◽  
Protein Extraction ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Fractionation Method ◽  
Metroxylon Sagu ◽  
Time Required

Abstract Objective This study aimed to determine the best protein extraction method of Metroxylon sagu for the two-dimensional gel electrophoresis and the comparative analysis. Results To perform good proteome research, the most critical step is to establish a method that gives the best quality of extracted total proteins. To develop an optimized protein extraction protocol for two-dimensional polyacrylamide gel electrophoresis (2-DE) analysis of Metroxylon sagu, five protein extraction protocols were compared; polyethene glycol (PEG) fractionation method, SDS/phenol method, TCA/acetone method, combination SDS/phenol and TCA/acetone and imidazole method. The PEG fractionation method was found to give the most reproducible gels with the highest number of spots and highest protein concentration followed by SDS/Phenol method. The lowest number of spots were observed in Imidazole method. The PEG fractionation method provides improved resolution and reproducibility of two-dimensional polyacrylamide gel electrophoresis (2-DE) and reduces the time required to analyze samples. Partitioning rubisco by polyethene glycol (PEG) fractionation provides clearer detection of low-abundance protein. Hence the result from this study propose PEG fractionation as the effective protein extraction method for 2-DE proteomic studies of Metroxylon sagu.

Wavelet Ridge Extraction Method Employing a Cost Function in Two-Dimensional Wavelet Transform Profilometry

2016 ◽  
Vol 36 (4) ◽  
pp. 0412006
Author(s):  
徐东瀛 Xu Dongying ◽  
李思坤 Li Sikun ◽  
王向朝 Wang Xiangzhao ◽  
汪凯巍 Wang Kaiwei
Keyword(s):  
Wavelet Transform ◽  
Cost Function ◽  
Extraction Method ◽  
Two Dimensional ◽  
Wavelet Ridge

scholarly journals A robust protein extraction method for two dimensional electrophoresis of silkworm proteins

2013 ◽  
Vol 04 (04) ◽  
pp. 584-589
Author(s):  
Lvgao Qin ◽  
Haifeng Shi ◽  
Hengchuan Xia ◽  
Yong Wang ◽  
Liang Chen ◽  
...  
Keyword(s):  
Extraction Method ◽  
Protein Extraction ◽  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Dimensional Electrophoresis
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