High resolution mass analysis of N- and C-terminal negative ions resulting from resonance electron capture by aliphatic amino acids

2010 ◽  
Vol 132 (23) ◽  
pp. 234306 ◽  
Author(s):  
Pavel V. Shchukin ◽  
Mars V. Muftakhov ◽  
Jeff Morré ◽  
Max L. Deinzer ◽  
Yury V. Vasil’ev
Keyword(s):  
Amino Acids ◽  
High Resolution ◽  
Electron Capture ◽  
Negative Ions ◽  
Mass Analysis ◽  
Resonance Electron Capture ◽  
High Resolution Mass ◽  
Resonance Electron ◽  
Resolution Mass

scholarly journals Identification of Auxin Metabolites in Brassicaceae by Ultra-Performance Liquid Chromatography Coupled with High-Resolution Mass Spectrometry

Molecules ◽  
2019 ◽  
Vol 24 (14) ◽  
pp. 2615 ◽  
Author(s):  
Panagiota-Kyriaki Revelou ◽  
Maroula G. Kokotou ◽  
Violetta Constantinou-Kokotou
Keyword(s):  
Mass Spectrometry ◽  
Amino Acids ◽  
Liquid Chromatography ◽  
High Resolution ◽  
Plant Extracts ◽  
High Resolution Mass Spectrometry ◽  
Ultra Performance Liquid Chromatography ◽  
Charge Ratio ◽  
High Resolution Mass ◽  
Resolution Mass

Auxins are signaling molecules involved in multiple stages of plant growth and development. The levels of the most important auxin, indole-3-acetic acid (IAA), are regulated by the formation of amide and ester conjugates with amino acids and sugars. In this work, IAA and IAA amide conjugates with amino acids bearing a free carboxylic group or a methyl ester group, along with some selected IAA metabolites, were studied in positive and negative electrospray ionization (ESI) modes, utilizing high-resolution mass spectrometry (HRMS) as a tool for their structural analysis. HRMS/MS spectra revealed the fragmentation patterns that enable us to identify IAA metabolites in plant extracts from eight vegetables of the Brassicaceae family using a fast and reliable ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-QToF-MS) method. The accurate m/z (mass to charge) ratio and abundance of the molecular and fragment ions of the studied compounds in plant extracts matched those obtained from commercially available or synthesized compounds and confirmed the presence of IAA metabolites.

scholarly journals Szentiamide, an N-formylated Cyclic Depsipeptide from Xenorhabdus szentirmaii DSM 16338T

2011 ◽  
Vol 6 (9) ◽  
pp. 1934578X1100600 ◽  
Author(s):  
Birgit Ohlendorf ◽  
Sven Simon ◽  
Jutta Wiese ◽  
Johannes F. Imhoff
Keyword(s):  
Mass Spectrometry ◽  
Amino Acids ◽  
High Resolution ◽  
Nmr Spectra ◽  
High Resolution Mass Spectrometry ◽  
Culture Broth ◽  
Two Dimensional ◽  
Cyclic Depsipeptide ◽  
High Resolution Mass ◽  
Resolution Mass

Szentiamide (1) a new cyclic hexadepsipeptide was isolated from the culture broth of the entomopathogenic bacterium Xenorhabdus szentirmaii DSM 16338T. The structure was elucidated by analysis of one- and two-dimensional NMR spectra and high resolution mass spectrometry. The amino acids were determined to be D-leucine, L-threonine, D-phenylalanine, D-valine, L-tyrosine and L-tryptophane after hydrolysis and derivatization with D-FDVA [ Nα-(2,4-dinitro-5-fluorophenyl)-D-valinamide].

Sign in / Sign up

Export Citation Format

Share Document