Picosecond‐laser‐pulse‐duration measurements with a low‐light‐level electro‐optic two‐photon fluorescence technique

1978 ◽  
Vol 49 (11) ◽  
pp. 5669-5671 ◽  
Author(s):  
J. Taboada ◽  
D. D. Venable
Keyword(s):  
Laser Pulse ◽  
Picosecond Laser ◽  
Light Level ◽  
Fluorescence Technique ◽  
Low Light ◽  
Picosecond Laser Pulse ◽  
Two Photon ◽  
Electro Optic ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

Single and two-photon fluorescence control of Er3+ ions by phase-shaped femtosecond laser pulse

2014 ◽  
Vol 104 (1) ◽  
pp. 014101 ◽  
Author(s):  
Shian Zhang ◽  
Shuwu Xu ◽  
Jingxin Ding ◽  
Chenhui Lu ◽  
Tianqing Jia ◽  
...  
Keyword(s):  
Laser Pulse ◽  
Femtosecond Laser ◽  
Femtosecond Laser Pulse ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

Two-photon fluorescence silica nanoparticles-based FRET nanoprobe platform for effectively ratiometric bioimaging of intracellular endogenous adenosine triphosphate

The Analyst ◽  
2021 ◽  
Author(s):  
Dian Li ◽  
Ying Liu ◽  
Shengrong Yu ◽  
Dailiang Zhang ◽  
Xiao Wang ◽  
...  
Keyword(s):  
Light Scattering ◽  
Silica Nanoparticles ◽  
Penetration Depth ◽  
Fluorescence Imaging ◽  
Adenosine Triphosphate ◽  
Low Light ◽  
Two Photon ◽  
Endogenous Adenosine ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

Two-photon fluorescence imaging is one of the most attractive imaging technologies for monitoring important biomolecule in biomedicine, due to its advantages of low light scattering, deeper penetration depth and suppressed...

scholarly journals Simultaneous Two-Photon Fluorescence Microscopy of NADH and FAD Using Pixel-to-Pixel Wavelength-Switching

2021 ◽  
Vol 9 ◽  
Author(s):  
Yifan Qin ◽  
Yuanqin Xia
Keyword(s):  
Biological Tissues ◽  
Wavelength Switching ◽  
Excitation Scheme ◽  
Excitation Efficiency ◽  
Two Photon ◽  
Electro Optic ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence ◽  
Bleed Through ◽  
Electro Optic Modulator

Two-photon fluorescence (TPF) microscopy of intrinsic fluorophores provides physiological and pathological information from biological tissues. Reduced nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FAD) are two endogenous fluorescent coenzymes existing on the intracellular scale. Autofluorescence images of NADH and FAD have been applied to noninvasively record changes during metabolism, according to their distributions and concentrations. However, the widely used sequential (non-simultaneous) excitation scheme results in artifacts caused by sample motion or laser power fluctuation. The single-wavelength illumination scheme suffers from low excitation efficiency and spectral bleed-through. In this paper, we demonstrate a new imaging system simultaneously capturing autofluorescence images from NADH and FAD, with high excitation efficiency and negligible spectral bleed-through. Two temporally multiplexed and spatially overlapped excitation beams were achieved with fast-switching light paths based on an electro-optic modulator. The switching beams were centered at 750 and 860 nm, enabling independent excitations of NADH and FAD. Autofluorescence images of NADH and FAD were acquired at the wavelength ranges of 415–455 nm and 500–550 nm, respectively. The electro-optic modulator was synchronized with the pixel clock from the microscope, achieving pixel-to-pixel wavelength-switching. The capability of the system was demonstrated by performing TPF imaging of freshly excised mouse colon tissues. The microenvironment of the colon wall was depicted by the distributions of colonocytes, goblet cells, and crypts of Lieberkühn, and the relative concentrations of NADH and FAD were estimated. The experimental results show that the system can effectively perform simultaneous imaging of NADH and FAD, and is considered a promising tool for investigations into metabolism-associated processes and diseases.

Experimental chaotic map generated by picosecond laser pulse-seeded electro-optic nonlinear delay dynamics

2008 ◽  
Vol 18 (1) ◽  
pp. 013110 ◽  
Author(s):  
Mélanie Grapinet ◽  
Vladimir Udaltsov ◽  
Maxime Jacquot ◽  
Pierre-Ambroise Lacourt ◽  
John M. Dudley ◽  
...  
Keyword(s):  
Laser Pulse ◽  
Chaotic Map ◽  
Picosecond Laser ◽  
Picosecond Laser Pulse ◽  
Electro Optic ◽  
Nonlinear Delay
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