scholarly journals Fluorescence correlation spectroscopy with sub-diffraction-limited resolution using near-field optical probes

2008 ◽  
Vol 93 (16) ◽  
pp. 163904 ◽  
Author(s):  
Dusan Vobornik ◽  
Daniel S. Banks ◽  
Zhengfang Lu ◽  
Cécile Fradin ◽  
Rod Taylor ◽  
...  
Keyword(s):  
Fluorescence Correlation Spectroscopy ◽  
Near Field ◽  
Correlation Spectroscopy ◽  
Optical Probes ◽  
Fluorescence Correlation

scholarly journals Fluorescence Correlation Spectroscopy with Sub-Diffraction-Limited Resolution Using Near-field Optical Probes

2009 ◽  
Vol 96 (3) ◽  
pp. 26a
Author(s):  
Linda J. Johnston ◽  
Dusan Vobornik ◽  
Zhengfang Lu ◽  
Rod Taylor ◽  
Daniel Banks ◽  
...  
Keyword(s):  
Fluorescence Correlation Spectroscopy ◽  
Near Field ◽  
Correlation Spectroscopy ◽  
Optical Probes ◽  
Fluorescence Correlation

scholarly journals Near-field optical probes provide subdiffraction-limited excitation areas for fluorescence correlation spectroscopy on membranes

2009 ◽  
Vol 81 (9) ◽  
pp. 1645-1653 ◽  
Author(s):  
Dusan Vobornik ◽  
Daniel S. Banks ◽  
Zhengfang Lu ◽  
Cécile Fradin ◽  
Rod Taylor ◽  
...  
Keyword(s):  
Fluorescence Correlation Spectroscopy ◽  
Near Field ◽  
Correlation Spectroscopy ◽  
Probe Design ◽  
Aqueous Environment ◽  
Optical Probes ◽  
Aperture Diameter ◽  
Fluorescence Correlation ◽  
Observation Area ◽  
Order Of Magnitude

Near-field optical probes have been used to produce a subdiffraction-limited observation area for fluorescence correlation spectroscopy (FCS) experiments on supported membranes. The design of a bent, etched fiber probe that is compatible with biological imaging in an aqueous environment is described. This probe design is used for proof of principle experiments to measure lipid diffusion in a fluid-supported bilayer. A reduction in excitation area of approximately one order of magnitude (relative to a confocal FCS experiment) is obtained with a probe aperture diameter of 140 nm. We also demonstrate a simple approach for modeling the autocorrelation decay due to diffusion within the excitation profile at the near-field scanning optical microscopy (NSOM) probe aperture. The use of probes with smaller apertures is expected to provide an additional order of magnitude reduction in the observation area, thus enabling the study of cellular membranes with higher concentrations of fluorophores than is currently possible with diffraction-limited techniques.

scholarly journals An Array of Planar Apertures for Near-Field Fluorescence Correlation Spectroscopy

2011 ◽  
Vol 100 (7) ◽  
pp. L34-L36 ◽  
Author(s):  
Christopher V. Kelly ◽  
Barbara A. Baird ◽  
Harold G. Craighead
Keyword(s):  
Fluorescence Correlation Spectroscopy ◽  
Near Field ◽  
Correlation Spectroscopy ◽  
Fluorescence Correlation

scholarly journals Near-field optical fluorescence correlation spectroscopy

2010 ◽  
Author(s):  
José Pérez ◽  
Michael Herrmann ◽  
Dietmar Gradl ◽  
Andreas Naber
Keyword(s):  
Fluorescence Correlation Spectroscopy ◽  
Near Field ◽  
Correlation Spectroscopy ◽  
Fluorescence Correlation ◽  
Optical Fluorescence

Plasmonic near-field in the vicinity of a single gold nanoparticle investigated with fluorescence correlation spectroscopy

Nanoscale ◽  
2012 ◽  
Vol 4 (11) ◽  
pp. 3359 ◽  
Author(s):  
Guowei Lu ◽  
Jie Liu ◽  
Tianyue Zhang ◽  
Wenqiang Li ◽  
Lei Hou ◽  
...  
Keyword(s):  
Gold Nanoparticle ◽  
Fluorescence Correlation Spectroscopy ◽  
Near Field ◽  
Correlation Spectroscopy ◽  
Fluorescence Correlation
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