Use of Stagnation Point Flows for DNA Trapping, Manipulation, and Target Sequence Detection

2008 ◽  
Author(s):  
Rebecca Dylla-Spears ◽  
Lydia L. Sohn ◽  
Susan J. Muller ◽  
Albert Co ◽  
Gary L. Leal ◽  
...  
Keyword(s):  
Stagnation Point ◽  
Target Sequence ◽  
Sequence Detection ◽  
Dna Trapping

scholarly journals A rapid, accurate, scalable, and portable testing system for COVID-19 diagnosis

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Guanhua Xun ◽  
Stephan Thomas Lane ◽  
Vassily Andrew Petrov ◽  
Brandon Elliott Pepa ◽  
Huimin Zhao
Keyword(s):  
Limit Of Detection ◽  
Low Cost ◽  
High Volume ◽  
N Gene ◽  
Testing System ◽  
Target Sequence ◽  
One Pot ◽  
Sequence Detection ◽  
Highly Sensitive ◽  
Speed Accuracy

AbstractThe need for rapid, accurate, and scalable testing systems for COVID-19 diagnosis is clear and urgent. Here, we report a rapid Scalable and Portable Testing (SPOT) system consisting of a rapid, highly sensitive, and accurate assay and a battery-powered portable device for COVID-19 diagnosis. The SPOT assay comprises a one-pot reverse transcriptase-loop-mediated isothermal amplification (RT-LAMP) followed by PfAgo-based target sequence detection. It is capable of detecting the N gene and E gene in a multiplexed reaction with the limit of detection (LoD) of 0.44 copies/μL and 1.09 copies/μL, respectively, in SARS-CoV-2 virus-spiked saliva samples within 30 min. Moreover, the SPOT system is used to analyze 104 clinical saliva samples and identified 28/30 (93.3% sensitivity) SARS-CoV-2 positive samples (100% sensitivity if LoD is considered) and 73/74 (98.6% specificity) SARS-CoV-2 negative samples. This combination of speed, accuracy, sensitivity, and portability will enable high-volume, low-cost access to areas in need of urgent COVID-19 testing capabilities.

scholarly journals Nanopore-Based Target Sequence Detection

PLoS ONE ◽  
2016 ◽  
Vol 11 (5) ◽  
pp. e0154426 ◽  
Author(s):  
Trevor J. Morin ◽  
Tyler Shropshire ◽  
Xu Liu ◽  
Kyle Briggs ◽  
Cindy Huynh ◽  
...  
Keyword(s):  
Target Sequence ◽  
Sequence Detection

scholarly journals Single-molecule sequence detection via microfluidic planar extensional flow at a stagnation point

Lab on a Chip ◽  
2010 ◽  
Vol 10 (12) ◽  
pp. 1543 ◽  
Author(s):  
Rebecca Dylla-Spears ◽  
Jacqueline E. Townsend ◽  
Linda Jen-Jacobson ◽  
Lydia L. Sohn ◽  
Susan J. Muller
Keyword(s):  
Stagnation Point ◽  
Single Molecule ◽  
Extensional Flow ◽  
Sequence Detection ◽  
Planar Extensional Flow
Sign in / Sign up

Export Citation Format

Share Document