Direct visualization of triplex DNA molecular dynamics by fluorescence resonance energy transfer and atomic force microscopy measurements

2007 ◽  
Vol 91 (20) ◽  
pp. 203901 ◽  
Author(s):  
Chia-Ching Chang ◽  
Po-Yen Lin ◽  
Yen-Fu Chen ◽  
Chia-Seng Chang ◽  
Lou-Sing Kan
Keyword(s):  
Molecular Dynamics ◽  
Atomic Force Microscopy ◽  
Energy Transfer ◽  
Fluorescence Resonance Energy Transfer ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Triplex Dna ◽  
Direct Visualization ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Protein O-Fucosyltransferase 1 Undergoes Interdomain Flexibility in Solution

Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2105
Author(s):  
Erandi Lira-Navarrete ◽  
María Carmen Pallarés ◽  
Fabio Castello ◽  
Maria J. Ruedas-Rama ◽  
Angel Orte ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Single Molecule ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Catalytic Cycle ◽  
Conformational Behavior ◽  
Single Molecule Fluorescence ◽  
Force Microscopy ◽  
Atomic Force ◽  
Dynamic Population

Protein O-fucosyltransferase 1 (PoFUT1) is a GT-B fold enzyme that fucosylates proteins containing EGF-like repeats. GT-B glycosyltransferases have shown a remarkable grade of plasticity adopting closed and open conformations as a way of tuning their catalytic cycle, a feature that has not been observed for PoFUT1. Here, we analyzed Caenorhabditis elegans PoFUT1 (CePoFUT1) conformational behavior in solution by atomic force microscopy (AFM) and single-molecule fluorescence resonance energy transfer (SMF-FRET). Our results show that this enzyme is very flexible and adopts mainly compact conformations and to a lesser extend a highly dynamic population that oscillates between compact and highly extended conformations. Overall, our experiments illustrate the inherent complexity of CePoFUT1 dynamics, which might play a role during its catalytic cycle.

scholarly journals Esterase 2 as a Fluorescent Biosensor for the Detection of Organophosphorus Compounds: Docking and Electronic Insights from Molecular Dynamics

2019 ◽  
Author(s):  
Ingrid G. Prandi ◽  
Teodorico Ramalho ◽  
Tanos C.C. França
Keyword(s):  
Molecular Dynamics ◽  
Energy Transfer ◽  
Computational Chemistry ◽  
Fluorescence Resonance Energy Transfer ◽  
Organophosphorus Compounds ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Rural Workers ◽  
Fluorescent Biosensor ◽  
Transfer Theory

<div>Organophosphorus compounds (OP) are mainly used in agriculture as pesticides. Unfortunately, each year many rural workers die intoxicated by those compounds. Sometimes the diagnosis of the exact molecule that caused the intoxication can be tardy. In this work, we have applied computational chemistry methodologies and Förster fluorescence resonance energy transfer theory to have a better understanding of the fluorescence profiles that are described in the literature. </div>

scholarly journals Esterase 2 as a Fluorescent Biosensor for the Detection of Organophosphorus Compounds: Docking and Electronic Insights from Molecular Dynamics

2019 ◽  
Author(s):  
Ingrid G. Prandi ◽  
Teodorico Ramalho ◽  
Tanos C.C. França
Keyword(s):  
Molecular Dynamics ◽  
Energy Transfer ◽  
Computational Chemistry ◽  
Fluorescence Resonance Energy Transfer ◽  
Organophosphorus Compounds ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Rural Workers ◽  
Fluorescent Biosensor ◽  
Transfer Theory

<div>Organophosphorus compounds (OP) are mainly used in agriculture as pesticides. Unfortunately, each year many rural workers die intoxicated by those compounds. Sometimes the diagnosis of the exact molecule that caused the intoxication can be tardy. In this work, we have applied computational chemistry methodologies and Förster fluorescence resonance energy transfer theory to have a better understanding of the fluorescence profiles that are described in the literature. </div>

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