Ultrasensitive, label-free, and real-time immunodetection using silicon field-effect transistors

2007 ◽  
Vol 91 (10) ◽  
pp. 103901 ◽  
Author(s):  
Ansoon Kim ◽  
Chil Seong Ah ◽  
Han Young Yu ◽  
Jong-Heon Yang ◽  
In-Bok Baek ◽  
...  
Keyword(s):  
Real Time ◽  
Field Effect ◽  
Field Effect Transistors ◽  
Label Free

Label-Free and Real-Time Detection of Avian Influenza Using Nanowire Field Effect Transistors

2015 ◽  
Vol 11 (9) ◽  
pp. 1640-1643 ◽  
Author(s):  
Jae-Hyuk Ahn ◽  
Maesoon Im ◽  
Tae Jung Park ◽  
Sang Yup Lee ◽  
Yang-Kyu Choi
Keyword(s):  
Avian Influenza ◽  
Real Time ◽  
Field Effect ◽  
Field Effect Transistors ◽  
Label Free ◽  
Real Time Detection

scholarly journals High-Throughput Peptide Epitope Mapping Using Carbon Nanotube Field-Effect Transistors

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Steingrimur Stefansson ◽  
Martha Knight ◽  
Hena H. Kwon ◽  
Lára A. Stefansson ◽  
Saeyoung Nate Ahn
Keyword(s):  
Carbon Nanotube ◽  
Real Time ◽  
High Throughput ◽  
Field Effect ◽  
Epitope Mapping ◽  
Field Effect Transistors ◽  
Peptide Library ◽  
Label Free ◽  
Peptide Epitope ◽  
Binding Data

Label-free and real-time detection technologies can dramatically reduce the time and cost of pharmaceutical testing and development. However, to reach their full promise, these technologies need to be adaptable to high-throughput automation. To demonstrate the potential of single-walled carbon nanotube field-effect transistors (SWCNT-FETs) for high-throughput peptide-based assays, we have designed circuits arranged in an 8 × 12 (96-well) format that are accessible to standard multichannel pipettors. We performed epitope mapping of two HIV-1 gp160 antibodies using an overlapping gp160 15-mer peptide library coated onto nonfunctionalized SWCNTs. The 15-mer peptides did not require a linker to adhere to the non-functionalized SWCNTs, and binding data was obtained in real time for all 96 circuits. Despite some sequence differences in the HIV strains used to generate these antibodies and the overlapping peptide library, respectively, our results using these antibodies are in good agreement with known data, indicating that peptides immobilized onto SWCNT are accessible and that linear epitope mapping can be performed in minutes using SWCNT-FET.

scholarly journals Biologically sensitive field-effect transistors: from ISFETs to NanoFETs

2016 ◽  
Vol 60 (1) ◽  
pp. 81-90 ◽  
Author(s):  
Vivek Pachauri ◽  
Sven Ingebrandt
Keyword(s):  
Field Effect ◽  
Gate Dielectric ◽  
Gate Dielectrics ◽  
Field Effect Transistors ◽  
Silicon Nanowire ◽  
Label Free ◽  
Biomolecular Detection ◽  
New Device ◽  
Label Free Detection ◽  
History Of

Biologically sensitive field-effect transistors (BioFETs) are one of the most abundant classes of electronic sensors for biomolecular detection. Most of the time these sensors are realized as classical ion-sensitive field-effect transistors (ISFETs) having non-metallized gate dielectrics facing an electrolyte solution. In ISFETs, a semiconductor material is used as the active transducer element covered by a gate dielectric layer which is electronically sensitive to the (bio-)chemical changes that occur on its surface. This review will provide a brief overview of the history of ISFET biosensors with general operation concepts and sensing mechanisms. We also discuss silicon nanowire-based ISFETs (SiNW FETs) as the modern nanoscale version of classical ISFETs, as well as strategies to functionalize them with biologically sensitive layers. We include in our discussion other ISFET types based on nanomaterials such as carbon nanotubes, metal oxides and so on. The latest examples of highly sensitive label-free detection of deoxyribonucleic acid (DNA) molecules using SiNW FETs and single-cell recordings for drug screening and other applications of ISFETs will be highlighted. Finally, we suggest new device platforms and newly developed, miniaturized read-out tools with multichannel potentiometric and impedimetric measurement capabilities for future biomedical applications.

NO2 Detection and Real-Time Sensing with Field-Effect Transistors

2013 ◽  
Vol 26 (1) ◽  
pp. 773-785 ◽  
Author(s):  
Anne-Marije Andringa ◽  
Claudia Piliego ◽  
Ilias Katsouras ◽  
Paul W. M. Blom ◽  
Dago M. de Leeuw
Keyword(s):  
Real Time ◽  
Field Effect ◽  
Field Effect Transistors ◽  
No2 Detection

Real-Time DNA Detection Using Reduced Graphene Oxide Field Effect Transistors

2010 ◽  
Vol 22 (46) ◽  
pp. 5297-5300 ◽  
Author(s):  
Rory Stine ◽  
Jeremy T. Robinson ◽  
Paul E. Sheehan ◽  
Cy R. Tamanaha
Keyword(s):  
Graphene Oxide ◽  
Real Time ◽  
Reduced Graphene Oxide ◽  
Field Effect ◽  
Field Effect Transistors ◽  
Dna Detection ◽  
Reduced Graphene

scholarly journals Real-time Monitoring of Cell Activities by Diamond Solution-gated Field Effect Transistors

2016 ◽  
Vol 168 ◽  
pp. 469-472 ◽  
Author(s):  
Tibor Ižák ◽  
Václav Procházka ◽  
Toshiya Sakata ◽  
Bohuslav Rezek ◽  
Alexander Kromka
Keyword(s):  
Real Time ◽  
Field Effect ◽  
Field Effect Transistors ◽  
Real Time Monitoring
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