scholarly journals Tip-sample distance control using photothermal actuation of a small cantilever for high-speed atomic force microscopy

2007 ◽  
Vol 78 (8) ◽  
pp. 083702 ◽  
Author(s):  
Hayato Yamashita ◽  
Noriyuki Kodera ◽  
Atsushi Miyagi ◽  
Takayuki Uchihashi ◽  
Daisuke Yamamoto ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
High Speed ◽  
Force Microscopy ◽  
Atomic Force ◽  
Distance Control ◽  
Sample Distance

scholarly journals Histone variant H2A.B-H2B dimers are spontaneously exchanged with canonical H2A-H2B in the nucleosome

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Rina Hirano ◽  
Yasuhiro Arimura ◽  
Tomoya Kujirai ◽  
Mikihiro Shibata ◽  
Aya Okuda ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Dna Repair ◽  
High Speed ◽  
Histone Chaperones ◽  
Histone H2a ◽  
Force Microscopy ◽  
Atomic Force ◽  
Open Conformation ◽  
Replication Sites ◽  
Histone Exchange

AbstractH2A.B is an evolutionarily distant histone H2A variant that accumulates on DNA repair sites, DNA replication sites, and actively transcribing regions in genomes. In cells, H2A.B exchanges rapidly in chromatin, but the mechanism has remained enigmatic. In the present study, we found that the H2A.B-H2B dimer incorporated within the nucleosome exchanges with the canonical H2A-H2B dimer without assistance from additional factors, such as histone chaperones and nucleosome remodelers. High-speed atomic force microscopy revealed that the H2A.B nucleosome, but not the canonical H2A nucleosome, transiently forms an intermediate “open conformation”, in which two H2A.B-H2B dimers may be detached from the H3-H4 tetramer and bind to the DNA regions near the entry/exit sites. Mutational analyses revealed that the H2A.B C-terminal region is responsible for the adoption of the open conformation and the H2A.B-H2B exchange in the nucleosome. These findings provide mechanistic insights into the histone exchange of the H2A.B nucleosome.

High-Speed Atomic Force Microscopy for Studying the Dynamic Behavior of Protein Molecules at Work

2006 ◽  
Vol 45 (3B) ◽  
pp. 1897-1903 ◽  
Author(s):  
Toshio Ando ◽  
Takayuki Uchihashi ◽  
Noriyuki Kodera ◽  
Atsushi Miyagi ◽  
Ryo Nakakita ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Dynamic Behavior ◽  
High Speed ◽  
Force Microscopy ◽  
Atomic Force ◽  
Protein Molecules

Direct observation of surfactant aggregate behavior on a mica surface using high-speed atomic force microscopy

2011 ◽  
Vol 47 (17) ◽  
pp. 4974 ◽  
Author(s):  
Shigeto Inoue ◽  
Takayuki Uchihashi ◽  
Daisuke Yamamoto ◽  
Toshio Ando
Keyword(s):  
Atomic Force Microscopy ◽  
Direct Observation ◽  
High Speed ◽  
Mica Surface ◽  
Force Microscopy ◽  
Atomic Force ◽  
Aggregate Behavior

Serum-induced degradation of 3D DNA box origami observed with high-speed atomic force microscopy

Nano Research ◽  
2015 ◽  
Vol 8 (7) ◽  
pp. 2170-2178 ◽  
Author(s):  
Zaixing Jiang ◽  
Shuai Zhang ◽  
Chuanxu Yang ◽  
Jørgen Kjems ◽  
Yudong Huang ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
High Speed ◽  
Force Microscopy ◽  
Atomic Force
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