Novel apparatus to measure hyperthermal heavy ion damage to DNA: Strand breaks, base loss, and fragmentation

2007 ◽  
Vol 78 (8) ◽  
pp. 085111 ◽  
Author(s):  
L. Sellami ◽  
S. Lacombe ◽  
D. Hunting ◽  
R. J. Wagner ◽  
M. A. Huels
Keyword(s):  
Heavy Ion ◽  
Dna Strand Breaks ◽  
Base Loss ◽  
Strand Breaks ◽  
Ion Damage ◽  
Dna Strand

scholarly journals Visualization of Heavy Ion Tracks by Labeling 3'-OH Termini of Induced DNA Strand Breaks

2011 ◽  
Vol 52 (4) ◽  
pp. 433-440 ◽  
Author(s):  
Teruaki KONISHI ◽  
Akihiro TAKEYASU ◽  
Toshiyuki NATSUME ◽  
Yoshiya FURUSAWA ◽  
Kotaro HIEDA
Keyword(s):  
Heavy Ion ◽  
Dna Strand Breaks ◽  
Strand Breaks ◽  
Ion Tracks ◽  
Dna Strand

scholarly journals Repair of DNA Strand Breaks by the Overlapping Functions of Lesion-Specific and Non-Lesion-Specific DNA 3′ Phosphatases

2001 ◽  
Vol 21 (21) ◽  
pp. 7191-7198 ◽  
Author(s):  
John R. Vance ◽  
Thomas E. Wilson
Keyword(s):  
Dna Damage ◽  
Oxidative Dna Damage ◽  
Synthetic Lethality ◽  
Dna Strand Breaks ◽  
Triple Mutant ◽  
Phosphatase Domain ◽  
Strand Breaks ◽  
Alternative Pathways ◽  
Processing Enzymes ◽  
Dna Strand

ABSTRACT In Saccharomyces cerevisiae, the apurinic/apyrimidinic (AP) endonucleases Apn1 and Apn2 act as alternative pathways for the removal of various 3′-terminal blocking lesions from DNA strand breaks and in the repair of abasic sites, which both result from oxidative DNA damage. Here we demonstrate that Tpp1, a homologue of the 3′ phosphatase domain of polynucleotide kinase, is a third member of this group of redundant 3′ processing enzymes. Unlike Apn1 and Apn2, Tpp1 is specific for the removal of 3′ phosphates at strand breaks and does not possess more general 3′ phosphodiesterase, exonuclease, or AP endonuclease activities. Deletion ofTPP1 in an apn1 apn2 mutant background dramatically increased the sensitivity of the double mutant to DNA damage caused by H2O2 and bleomycin but not to damage caused by methyl methanesulfonate. The triple mutant was also deficient in the repair of 3′ phosphate lesions left by Tdp1-mediated cleavage of camptothecin-stabilized Top1-DNA covalent complexes. Finally, the tpp1 apn1 apn2 triple mutation displayed synthetic lethality in combination with rad52, possibly implicating postreplication repair in the removal of unrepaired 3′-terminal lesions resulting from endogenous damage. Taken together, these results demonstrate a clear role for the lesion-specific enzyme, Tpp1, in the repair of a subset of DNA strand breaks.

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