Nondestructive micropatterning of living animal cells using focused femtosecond laser-induced impulsive force

2007 ◽  
Vol 91 (2) ◽  
pp. 023904 ◽  
Author(s):  
Takahiro Kaji ◽  
Syoji Ito ◽  
Hiroshi Miyasaka ◽  
Yoichiroh Hosokawa ◽  
Hiroshi Masuhara ◽  
...  
Keyword(s):  
Femtosecond Laser ◽  
Living Animal ◽  
Impulsive Force ◽  
Animal Cells

Nanoparticle injection to single animal cells using femtosecond laser-induced impulsive force

2008 ◽  
Vol 93 (1) ◽  
pp. 39-43 ◽  
Author(s):  
Atsushi Yamaguchi ◽  
Yoichiroh Hosokawa ◽  
Guillaume Louit ◽  
Tsuyoshi Asahi ◽  
Chisa Shukunami ◽  
...  
Keyword(s):  
Femtosecond Laser ◽  
Impulsive Force ◽  
Animal Cells ◽  
Single Animal

scholarly journals Translation of plant-specific messenger RNAs in living animal cells

FEBS Letters ◽  
1977 ◽  
Vol 81 (1) ◽  
pp. 10-12 ◽  
Author(s):  
J. Schröder ◽  
F. Kreuzaler ◽  
J. Schmock
Keyword(s):  
Messenger Rnas ◽  
Living Animal ◽  
Animal Cells

Nondestructive isolation of single cultured animal cells by femtosecond laser-induced shockwave

2004 ◽  
Vol 79 (4-6) ◽  
pp. 795-798 ◽  
Author(s):  
Y. Hosokawa ◽  
H. Takabayashi ◽  
S. Miura ◽  
C. Shukunami ◽  
Y. Hiraki ◽  
...  
Keyword(s):  
Femtosecond Laser ◽  
Animal Cells

Mechanical response of single nerve cells estimated by femtosecond laser-induced impulsive force

2015 ◽  
Author(s):  
Takanori Iino ◽  
Tadahide Furuno ◽  
Man Hagiyama ◽  
Akihiko Ito ◽  
Yoichiroh Hosokawa
Keyword(s):  
Femtosecond Laser ◽  
Mechanical Response ◽  
Nerve Cells ◽  
Impulsive Force ◽  
Single Nerve

scholarly journals Direct observation of branching MT nucleation in living animal cells

2019 ◽  
Vol 218 (9) ◽  
pp. 2829-2840 ◽  
Author(s):  
Vikash Verma ◽  
Thomas J. Maresca
Keyword(s):  
Direct Observation ◽  
Branch Point ◽  
Living Animal ◽  
Nucleation Event ◽  
Animal Cells ◽  
Rhoa Activation ◽  
Branch Angle ◽  
Nucleation Mechanisms ◽  
Ring Complex ◽  
Drosophila Cells

Centrosome-mediated microtubule (MT) nucleation has been well characterized; however, numerous noncentrosomal MT nucleation mechanisms exist. The branching MT nucleation pathway envisages that the γ-tubulin ring complex (γ-TuRC) is recruited to MTs by the augmin complex to initiate nucleation of new MTs. While the pathway is well conserved at a molecular and functional level, branching MT nucleation by core constituents has never been directly observed in animal cells. Here, multicolor TIRF microscopy was applied to visualize and quantitatively define the entire process of branching MT nucleation in dividing Drosophila cells during anaphase. The steps of a stereotypical branching nucleation event entailed augmin binding to a mother MT and recruitment of γ-TuRC after 15 s, followed by nucleation 16 s later of a daughter MT at a 36° branch angle. Daughters typically remained attached throughout their ∼40-s lifetime unless the mother depolymerized past the branch point. Assembly of branched MT arrays, which did not require Drosophila TPX2, enhanced localized RhoA activation during cytokinesis.

Morphological evaluation of cell differentiation after the isolation of single cells by a femtosecond laser-induced impulsive force

2010 ◽  
Vol 13 (1) ◽  
pp. 117-122 ◽  
Author(s):  
Yasuyo Maezawa ◽  
Kazunori Okano ◽  
Mie Matsubara ◽  
Hiroshi Masuhara ◽  
Yoichiroh Hosokawa
Keyword(s):  
Cell Differentiation ◽  
Femtosecond Laser ◽  
Single Cells ◽  
Impulsive Force ◽  
Morphological Evaluation

Dicistronic selection for nuclear proteins in living animal cells

Gene ◽  
1993 ◽  
Vol 137 (1) ◽  
pp. 145-149 ◽  
Author(s):  
an Smarda ◽  
Joseph S. Lipsick
Keyword(s):  
Nuclear Proteins ◽  
Living Animal ◽  
Animal Cells ◽  
Selection For
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