Nanodimentional Aggregates In Organic Monolayers Studied With Atomic Force Microscopy (AFM) And Fluorescence Lifetime Imaging Microscopy (FLIM)

2007 ◽  
Author(s):  
George R. Ivanov ◽  
Julian Burov
Keyword(s):  
Atomic Force Microscopy ◽  
Fluorescence Lifetime ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Organic Monolayers ◽  
Force Microscopy ◽  
Lifetime Imaging ◽  
Atomic Force

scholarly journals Synchronous, Crosstalk-free Correlative AFM and Confocal Microscopies/Spectroscopies

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Thales F. D. Fernandes ◽  
Oscar Saavedra-Villanueva ◽  
Emmanuel Margeat ◽  
Pierre-Emmanuel Milhiet ◽  
Luca Costa
Keyword(s):  
Fluorescence Lifetime ◽  
Interaction Force ◽  
Metallic Surface ◽  
Fluorescence Lifetime Imaging ◽  
Time Resolved ◽  
Force Microscopy ◽  
Lifetime Imaging ◽  
Atomic Force ◽  
Dimensional Mapping ◽  
Time Acquisition

Abstract Microscopies have become pillars of our characterization tools to observe biological systems and assemblies. Correlative and synchronous use of different microscopies relies on the fundamental assumption of non-interference during images acquisitions. In this work, by exploring the correlative use of Atomic Force Microscopy and confocal-Fluorescence-Lifetime Imaging Microscopy (AFM-FLIM), we quantify cross-talk effects occurring during synchronous acquisition. We characterize and minimize optomechanical forces on different AFM cantilevers interfering with normal AFM operation as well as spurious luminescence from the tip and cantilever affecting time-resolved fluorescence detection. By defining non-interfering experimental imaging parameters, we show accurate real-time acquisition and two-dimensional mapping of interaction force, fluorescence lifetime and intensity characterizing morphology (AFM) and local viscosity (FLIM) of gel and fluid phases separation of supported lipid model membranes. Finally, as proof of principle by means of synchronous force and fluorescence spectroscopies, we precisely tune the lifetime of a fluorescent nanodiamond positioned on the AFM tip by controlling its distance from a metallic surface. This opens up a novel pathway of quench sensing to image soft biological samples such as membranes since it does not require tip-sample mechanical contact in contrast with conventional AFM in liquid.

scholarly journals Direct organelle thermometry with fluorescence lifetime imaging microscopy in single myotubes

2016 ◽  
Vol 52 (24) ◽  
pp. 4458-4461 ◽  
Author(s):  
Hideki Itoh ◽  
Satoshi Arai ◽  
Thankiah Sudhaharan ◽  
Sung-Chan Lee ◽  
Young-Tae Chang ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Heat Production ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
C2c12 Myotube

FLIM of ER thermo yellow and non-targeted mCherry reveals the Ca2+-dependent heat production localized to SR in C2C12 myotube.

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