Simultaneous detection and identification of multigas pollutants using filament-induced nonlinear spectroscopy

2007 ◽  
Vol 90 (10) ◽  
pp. 101106 ◽  
Author(s):  
H. L. Xu ◽  
Y. Kamali ◽  
C. Marceau ◽  
P. T. Simard ◽  
W. Liu ◽  
...  
Keyword(s):  
Simultaneous Detection ◽  
Nonlinear Spectroscopy ◽  
Detection And Identification

A new one-step multiplex PCR assay for simultaneous detection and identification of avian haemosporidian parasites

2018 ◽  
Vol 118 (1) ◽  
pp. 191-201 ◽  
Author(s):  
Arif Ciloglu ◽  
Vincenzo A. Ellis ◽  
Rasa Bernotienė ◽  
Gediminas Valkiūnas ◽  
Staffan Bensch
Keyword(s):  
Multiplex Pcr ◽  
Simultaneous Detection ◽  
Pcr Assay ◽  
Haemosporidian Parasites ◽  
Multiplex Pcr Assay ◽  
Detection And Identification ◽  
One Step

Multiplex polymerase chain reaction for simultaneous detection and identification of Bursaphelenchus xylophilus, B. mucronatus and B. fraudulentus – three closely related species within the xylophilus group

Nematology ◽  
2017 ◽  
Vol 19 (9) ◽  
pp. 1107-1116 ◽  
Author(s):  
Anna Filipiak ◽  
Przemysław Wieczorek ◽  
Marek Tomalak
Keyword(s):  
Multiplex Pcr ◽  
Simultaneous Detection ◽  
Morphological Characters ◽  
Bursaphelenchus Xylophilus ◽  
Chain Reaction ◽  
Closely Related Species ◽  
Detection And Identification ◽  
Different Populations ◽  
Polymerase Chain ◽  
Simultaneous Identification

Differentiation between Bursaphelenchus xylophilus and other related, non-pathogenic species can be ambiguous when based exclusively on morphological characters. The morphology of B. mucronatus and B. fraudulentus most closely resembles that of B. xylophilus. Moreover, all of these nematodes are found in both Asia and Europe and can colonise various species of pine. Therefore, for phytosanitary purposes it is necessary to identify the three species precisely and rapidly. We report the results of a multiplex PCR that utilises four primers to identify and discriminate the three Bursaphelenchus species simultaneously. The multiplex PCR yielded DNA fragments of 767, 305 and 132 bp, for B. xylophilus, B. mucronatus and B. fraudulentus, respectively. This primer combination has produced reliable results in multiplex PCR assays with a number of different populations of the listed species, and no cross-reactions were observed with other Bursaphelenchus species. The described approach is simple, reliable and cheaper than other molecular methods presently used for simultaneous identification of the above three species within the xylophilus group.

scholarly journals Clinical Accuracy of a PLEX-ID Flu Device for Simultaneous Detection and Identification of Influenza Viruses A and B

2012 ◽  
Vol 51 (1) ◽  
pp. 40-45 ◽  
Author(s):  
Yi-Wei Tang ◽  
Kristin S. Lowery ◽  
Alexandra Valsamakis ◽  
Virginia C. Schaefer ◽  
James D. Chappell ◽  
...  
Keyword(s):  
Simultaneous Detection ◽  
Influenza Viruses ◽  
Detection And Identification ◽  
Clinical Accuracy

scholarly journals Simultaneous Detection and Identification of Human Parainfluenza Viruses 1, 2, and 3 from Clinical Samples by Multiplex PCR

1998 ◽  
Vol 36 (5) ◽  
pp. 1388-1391 ◽  
Author(s):  
Juan E. Echevarría ◽  
Dean D. Erdman ◽  
Ella M. Swierkosz ◽  
Brian P. Holloway ◽  
Larry J. Anderson
Keyword(s):  
Simultaneous Detection ◽  
Clinical Samples ◽  
Rt Pcr ◽  
Negative Results ◽  
Parainfluenza Viruses ◽  
Detection And Identification ◽  
Serotype 1 ◽  
Pcr Assays ◽  
Syncytial Virus ◽  
Human Parainfluenza Viruses

Reverse transcription (RT)-PCR assays have been widely described for use in the diagnosis of human parainfluenza viruses (HPIVs) and other respiratory virus pathogens. However, these assays are mostly monospecific, requiring separate amplifications for each HPIV type. In the present work, we describe multiplex RT-PCR assays that detect and differentiate HPIV serotypes 1, 2, and 3 in a combined reaction. Specifically, a mixture of three pairs of primers to conserved regions of the hemagglutinin-neuraminidase gene of each HPIV serotype was used for primary amplification, yielding amplicons with similar sizes. For typing, a second amplification was performed with a mixture of nested primers, yielding amplicons with sizes easily differentiated by agarose gel electrophoresis. A modified single-amplification RT-PCR assay with fluorescence-labeled nested primers, followed by analysis of the labeled products on an automated sequencing gel, was also evaluated. Fifteen temporally and geographically diverse HPIV isolates from the Centers for Disease Control and Prevention archives and 26 of 30 (87%) previously positive nasopharyngeal specimens (8 of 10 positive for HPIV serotype 1 [HPIV1], 9 of 10 positive for HPIV2, and 9 of 10 positive for HPIV3) were positive and were correctly typed by both assays. Negative results were obtained with naso- or oropharyngeal specimens and/or culture isolates of 33 unrelated respiratory tract pathogens, including HPIV4, enterovirus, rhinovirus, respiratory syncytial virus, adenovirus, influenza virus, and Streptococcus pneumoniae. Our multiplex RT-PCR assays provide sensitive, specific, and simplified tools for the rapid diagnosis of HPIV infections.

scholarly journals One-Step Multiplex PCR for Simultaneous Detection and Identification of Eight Medically Important <i>Candida</i> Species

2021 ◽  
Vol 11 (01) ◽  
pp. 14-24
Author(s):  
Akira Fukatsu ◽  
Osamu Tsuzukibashi ◽  
Hidenori Suzuk ◽  
Katsuhiro Asaka ◽  
Yoshinori Ono ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Simultaneous Detection ◽  
Detection And Identification ◽  
One Step
Sign in / Sign up

Export Citation Format

Share Document