Amorphous/nanocrystalline silicon biosensor for the specific identification of unamplified nucleic acid sequences using gold nanoparticle probes

2007 ◽  
Vol 90 (2) ◽  
pp. 023903 ◽  
Author(s):  
Rodrigo Martins ◽  
Pedro Baptista ◽  
Leandro Raniero ◽  
Gonçalo Doria ◽  
Leonardo Silva ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Gold Nanoparticle ◽  
Nanocrystalline Silicon ◽  
Specific Identification ◽  
Nanoparticle Probes ◽  
Nucleic Acid Sequences

Disposable Nucleic Acid Biosensors Based on Gold Nanoparticle Probes and Lateral Flow Strip

2009 ◽  
Vol 81 (4) ◽  
pp. 1660-1668 ◽  
Author(s):  
Xun Mao ◽  
Yunqing Ma ◽  
Aiguo Zhang ◽  
Lurong Zhang ◽  
Lingwen Zeng ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Gold Nanoparticle ◽  
Lateral Flow ◽  
Lateral Flow Strip ◽  
Nanoparticle Probes

Gold nanoparticle probes for the detection of nucleic acid targets

2006 ◽  
Vol 363 (1-2) ◽  
pp. 120-126 ◽  
Author(s):  
C. Shad Thaxton ◽  
Dimitra G. Georganopoulou ◽  
Chad A. Mirkin
Keyword(s):  
Nucleic Acid ◽  
Gold Nanoparticle ◽  
Nanoparticle Probes

Gold-Aptamer-Nanoconstructs Engineered to Detect Conserved Enteroviral Nucleic Acid Sequences

2019 ◽  
Author(s):  
Veeren Chauhan ◽  
Mohamed M Elsutohy ◽  
C Patrick McClure ◽  
Will Irving ◽  
Neil Roddis ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
In Silico ◽  
Point Of Care ◽  
Lateral Flow ◽  
Nucleic Acid Sequence ◽  
In Silico Screening ◽  
Lateral Flow Assays ◽  
Life Threatening ◽  
Software And Hardware ◽  
Nucleic Acid Sequences

<p>Enteroviruses are a ubiquitous mammalian pathogen that can produce mild to life-threatening disease. Bearing this in mind, we have developed a rapid, accurate and economical point-of-care biosensor that can detect a nucleic acid sequences conserved amongst 96% of all known enteroviruses. The biosensor harnesses the physicochemical properties of gold nanoparticles and aptamers to provide colourimetric, spectroscopic and lateral flow-based identification of an exclusive enteroviral RNA sequence (23 bases), which was identified through in silico screening. Aptamers were designed to demonstrate specific complementarity towards the target enteroviral RNA to produce aggregated gold-aptamer nanoconstructs. Conserved target enteroviral nucleic acid sequence (≥ 1x10<sup>-7</sup> M, ≥1.4×10<sup>-14</sup> g/mL), initiates gold-aptamer-nanoconstructs disaggregation and a signal transduction mechanism, producing a colourimetric and spectroscopic blueshift (544 nm (purple) > 524 nm (red)). Furthermore, lateral-flow-assays that utilise gold-aptamer-nanoconstructs were unaffected by contaminating human genomic DNA, demonstrated rapid detection of conserved target enteroviral nucleic acid sequence (< 60 s) and could be interpreted with a bespoke software and hardware electronic interface. We anticipate our methodology will translate in-silico screening of nucleic acid databases to a tangible enteroviral desktop detector, which could be readily translated to related organisms. This will pave-the-way forward in the clinical evaluation of disease and complement existing strategies at overcoming antimicrobial resistance.</p>

scholarly journals Structural motifs and intramolecular interactions in non-canonical G-quadruplexes

2021 ◽  
Author(s):  
Jagannath Jana ◽  
Swantje Mohr ◽  
Yoanes Maria Vianney ◽  
Klaus Weisz
Keyword(s):  
Nucleic Acid ◽  
Structural Features ◽  
Intramolecular Interactions ◽  
Structural Motifs ◽  
Nucleic Acid Sequences

G-rich nucleic acid sequences encompassing G-tracts of varying lengths can fold into different non-canonical G-quadruplexes with distinct structural features.

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