scholarly journals Enhancement factor distribution around a single surface-enhanced Raman scattering hot spot and its relation to single molecule detection

2006 ◽  
Vol 125 (20) ◽  
pp. 204701 ◽  
Author(s):  
E. C. Le Ru ◽  
P. G. Etchegoin ◽  
M. Meyer
Keyword(s):  
Raman Scattering ◽  
Single Molecule ◽  
Enhancement Factor ◽  
Hot Spot ◽  
Surface Enhanced Raman Scattering ◽  
Single Molecule Detection ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering

Structural Dynamics of a Single Photoreceptor Protein Molecule Monitored With Surface-Enhanced Raman Scattering Substrates

2008 ◽  
Vol 1077 ◽  
Author(s):  
Kushagra Singhal ◽  
Karthik Bhatt ◽  
Zhouyang Kang ◽  
Wouter Hoff ◽  
Aihua Xie ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Single Molecule ◽  
Enhancement Factor ◽  
Protein Molecule ◽  
Surface Enhanced Raman Scattering ◽  
Initial Study ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering ◽  
Halorhodospira Halophila

ABSTRACTPhotoactive yellow protein (PYP) is a small cytosolic photoreceptor that actuates the negative phototactic response in its host organism Halorhodospira halophila. It has an optical absorption maximum at 446 nm (blue light). We report an initial study of the photocycle of PYP at the single molecule level using “high enhancement factor” surface-enhanced Raman scattering (SERS)-active nanostructures with 514 nm laser excitation. The SERS-active “nanometal-on-semiconductor” structures are prepared employing a redox technique on thin germanium films, coated on glass slides. Single molecule spectra are observed in terms of sudden appearance of discernable Raman peaks with spectral fluctuations. The single molecule spectra capture protonation, photo-isomerization, and H-bond breaking - the steps that are instrumental in the photocycle of PYP. This is indicative of single PYP molecules diffusing to high-enhancement-factor SERS sites, and undergoing photo-cycle under 514 nm excitation.

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