Selective excitation of tryptophan fluorescence decay in proteins using a subnanosecond 295nm light-emitting diode and time-correlated single-photon counting

2005 ◽  
Vol 86 (26) ◽  
pp. 261911 ◽  
Author(s):  
Colin D. McGuinness ◽  
Kulwinder Sagoo ◽  
David McLoskey ◽  
David J. S. Birch
Keyword(s):  
Single Photon ◽  
Light Emitting Diode ◽  
Photon Counting ◽  
Tryptophan Fluorescence ◽  
Selective Excitation ◽  
Fluorescence Decay ◽  
Single Photon Counting ◽  
Light Emitting

Picosecond Tryptophan Fluorescence of Human Blood Serum Orosomucoid

1996 ◽  
Vol 61 (5) ◽  
pp. 808-818 ◽  
Author(s):  
Martin Hof ◽  
Stefan Vajda ◽  
Vlastimil Fidler ◽  
Vladimír Karpenko
Keyword(s):  
Single Photon ◽  
Global Analysis ◽  
Photon Counting ◽  
Tryptophan Fluorescence ◽  
Fluorescence Decay ◽  
Amino Acid Residues ◽  
Counting System ◽  
Single Photon Counting ◽  
Decay Behaviour ◽  
The Individual

The state of three tryptophyl residues in human serum orosomucoid was estimated by prediction methods based on parameters characterizing their hydrophobicity either directly, or in terms of buried surfaces of the individual amino acid residues. It is shown that tryptophan 25 is the most buried, while Trp 160 is the most exposed to the solvent. Trp 122 is in this respect in an intermediate state. The fluorescence decay behaviour was determined using a picosecond single photon counting system. The multiwavelength data were analyzed using a global analysis as well as a distribution of lifetimes program. Both procedures yielded the existence of four wavelength independent lifetimes (0.22 ns, 1.0 ns, 2.5 ns, and 8.4 ns). A tentative assignment of the decay associated spectra of the four components to the three individual tryptophans is presented.

Single Particle Spectroscopy of Radiative Processes in Colloid-to-Film-Coupled Nanoantennas

2018 ◽  
Vol 232 (9-11) ◽  
pp. 1593-1606 ◽  
Author(s):  
Max J. Schnepf ◽  
Yannic Brasse ◽  
Fabian R. Goßler ◽  
Anja Maria Steiner ◽  
Julian Obermeier ◽  
...  
Keyword(s):  
Single Particle ◽  
Radiative Decay ◽  
Single Photon ◽  
Photon Counting ◽  
Life Time ◽  
Fluorescence Decay ◽  
Decay Rates ◽  
Single Photon Counting ◽  
Radiative Processes ◽  
Particle Level

Abstract We present a fluorescent emitter (rhodamine B) coupled to a dielectric or metallic interface as well as a metallic cavity to study their radiative decay processes. Supported by finite-difference time-domain (FDTD) simulations, we correlate the non-radiative and radiative decay rates with the absorption and scattering cross section efficiencies, respectively. On a single particle level, we use atomic force microscopy (AFM), scanning electron microscopy (SEM), scattering spectroscopy, fluorescence life time imaging (FLIM) and time-correlated single photon counting (TCSPC) to evaluate the enhanced fluorescence decay at the same location. With this study, we show a colloidal gain material, which can be integrated into lattices using existing directed self-assembled methods to study their coherent energy transfer.

scholarly journals Investigation of a single-photon hybrid emitting system based on NV-centers in nanodiamonds integrated with GaP NWs

2021 ◽  
Vol 2086 (1) ◽  
pp. 012142
Author(s):  
A S Goltaev ◽  
A M Mozharov ◽  
V V Yaroshenko ◽  
D A Zuev ◽  
I S Mukhin
Keyword(s):  
Molecular Beam ◽  
Quantum Communication ◽  
Single Photon ◽  
Photon Counting ◽  
Single Photon Counting ◽  
Light Emitting ◽  
Optical Modes ◽  
Photon Counting Method ◽  
Nv Centers

Abstract NV-centers can be used for quantum informatics, quantum communication and quantum sensing. The calculation of optical modes formed in a GaP cylindrical nanocavity covered by nanodiamonds has been performed. GaP nanowires have been synthesized with molecular beam epitaxy and played the role of optical resonators for light-emitting centers on the base of nanodiamonds with NV-centers. The optical characteristics of the GaP-based nanocavity were analyzed. The increase in the rate of spontaneous emission of NV-centers optically coupled to the nanocavity was estimated by the time correlated single photon counting method.

Measurement of fluorescence decay curves by the single photon counting method

1981 ◽  
Vol 34 (2) ◽  
pp. 165-168
Author(s):  
A. B. Evdokimov ◽  
G. V. Lukomskii ◽  
B. S. Neporent ◽  
O. V. Stolbova
Keyword(s):  
Single Photon ◽  
Photon Counting ◽  
Fluorescence Decay ◽  
Counting Method ◽  
Single Photon Counting ◽  
Photon Counting Method
Sign in / Sign up

Export Citation Format

Share Document