Automated tracking and laser micromanipulation of motile cells

2005 ◽  
Vol 76 (3) ◽  
pp. 035105 ◽  
Author(s):  
B. Stuhrmann ◽  
M. Gögler ◽  
T. Betz ◽  
A. Ehrlicher ◽  
D. Koch ◽  
...  
Keyword(s):  
Automated Tracking ◽  
Motile Cells ◽  
Laser Micromanipulation

Modulated polarization microscopy displays the dynamics of cytoskeletal structures in living, motile cells

1995 ◽  
Vol 53 ◽  
pp. 902-903
Author(s):  
J. R. Kuhn ◽  
M. Poenie
Keyword(s):  
Mitotic Spindle ◽  
Actin Filaments ◽  
Cell Shape ◽  
Dynamic Structure ◽  
Living Cells ◽  
Cytoskeletal Proteins ◽  
Polarization Microscopy ◽  
Video Enhancement ◽  
Ultrastructural Studies ◽  
Motile Cells

Cell shape and movement are controlled by elements of the cytoskeleton including actin filaments an microtubules. Unfortunately, it is difficult to visualize the cytoskeleton in living cells and hence follow it dynamics. Immunofluorescence and ultrastructural studies of fixed cells while providing clear images of the cytoskeleton, give only a static picture of this dynamic structure. Microinjection of fluorescently Is beled cytoskeletal proteins has proved useful as a way to follow some cytoskeletal events, but long terry studies are generally limited by the bleaching of fluorophores and presence of unassembled monomers.Polarization microscopy has the potential for visualizing the cytoskeleton. Although at present, it ha mainly been used for visualizing the mitotic spindle. Polarization microscopy is attractive in that it pro vides a way to selectively image structures such as cytoskeletal filaments that are birefringent. By combing ing standard polarization microscopy with video enhancement techniques it has been possible to image single filaments. In this case, however, filament intensity depends on the orientation of the polarizer and analyzer with respect to the specimen.

Development of Automated Tracking System with Active Cameras for Figure Skating

2011 ◽  
Vol 131 (4) ◽  
pp. 565-571
Author(s):  
Tomohiko Haraguchi ◽  
Tsuyoshi Taki ◽  
Junichi Hasegawa
Keyword(s):  
Tracking System ◽  
Figure Skating ◽  
Automated Tracking

MAMAS: Supporting Parent--Child Mealtime Interactions Using Automated Tracking and Speech Recognition

2020 ◽  
Vol 4 (CSCW1) ◽  
pp. 1-32
Author(s):  
Eunkyung Jo ◽  
Hyeonseok Bang ◽  
Myeonghan Ryu ◽  
Eun Jee Sung ◽  
Sungmook Leem ◽  
...  
Keyword(s):  
Speech Recognition ◽  
Automated Tracking ◽  
Mealtime Interactions ◽  
Parent Child

scholarly journals NaCl Promotes the Efficient Formation of Haematococcus pluvialis Nonmotile Cells under Phosphorus Deficiency

Marine Drugs ◽  
2021 ◽  
Vol 19 (6) ◽  
pp. 337
Author(s):  
Feng Li ◽  
Ning Zhang ◽  
Yulei Zhang ◽  
Qingsheng Lian ◽  
Caiying Qin ◽  
...  
Keyword(s):  
Haematococcus Pluvialis ◽  
Phosphorus Deficiency ◽  
Cell Formation ◽  
Animal Nutrition ◽  
Cell Factory ◽  
Cell Type ◽  
Negative Effects ◽  
Related Factors ◽  
Motile Cells ◽  
Application Prospects

Natural astaxanthin helps reduce the negative effects caused by oxidative stress and other related factors, thereby minimizing oxidative damage. Therefore, it has considerable potential and broad application prospects in human health and animal nutrition. Haematococcus pluvialis is considered to be the most promising cell factory for the production of natural astaxanthin. Previous studies have confirmed that nonmotile cells of H. pluvialis are more tolerant to high intensity of light than motile cells. Cultivating nonmotile cells as the dominant cell type in the red stage can significantly increase the overall astaxanthin productivity. However, we know very little about how to induce nonmotile cell formation. In this work, we first investigated the effect of phosphorus deficiency on the formation of nonmotile cells of H. pluvialis, and then investigated the effect of NaCl on the formation of nonmotile cells under the conditions of phosphorus deficiency. The results showed that, after three days of treatment with 0.1% NaCl under phosphorus deficiency, more than 80% of motile cells had been transformed into nonmotile cells. The work provides the most efficient method for the cultivation of H. pluvialis nonmotile cells so far, and it significantly improves the production of H. pluvialis astaxanthin.

scholarly journals Cell migration guided by long-lived spatial memory

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Joseph d’Alessandro ◽  
Alex Barbier--Chebbah ◽  
Victor Cellerin ◽  
Olivier Benichou ◽  
René Marc Mège ◽  
...  
Keyword(s):  
Cell Migration ◽  
Spatial Memory ◽  
Large Scale ◽  
Single Cells ◽  
Environmental Perturbations ◽  
Motile Cells ◽  
Unicellular Organisms ◽  
Biochemical Signals ◽  
The Impact ◽  
Cell Trajectories

AbstractLiving cells actively migrate in their environment to perform key biological functions—from unicellular organisms looking for food to single cells such as fibroblasts, leukocytes or cancer cells that can shape, patrol or invade tissues. Cell migration results from complex intracellular processes that enable cell self-propulsion, and has been shown to also integrate various chemical or physical extracellular signals. While it is established that cells can modify their environment by depositing biochemical signals or mechanically remodelling the extracellular matrix, the impact of such self-induced environmental perturbations on cell trajectories at various scales remains unexplored. Here, we show that cells can retrieve their path: by confining motile cells on 1D and 2D micropatterned surfaces, we demonstrate that they leave long-lived physicochemical footprints along their way, which determine their future path. On this basis, we argue that cell trajectories belong to the general class of self-interacting random walks, and show that self-interactions can rule large scale exploration by inducing long-lived ageing, subdiffusion and anomalous first-passage statistics. Altogether, our joint experimental and theoretical approach points to a generic coupling between motile cells and their environment, which endows cells with a spatial memory of their path and can dramatically change their space exploration.

epiTracker: A Framework for Highly Reliable Particle Tracking for the Quantitative Analysis of Fish Movements in Tanks

2020 ◽  
pp. 247263032097745
Author(s):  
Roman Bruch ◽  
Paul M. Scheikl ◽  
Ralf Mikut ◽  
Felix Loosli ◽  
Markus Reischl
Keyword(s):  
Social Interactions ◽  
User Interfaces ◽  
Particle Tracking ◽  
Group Behavior ◽  
Correction Method ◽  
Graphical User Interfaces ◽  
Study Group ◽  
Automated Tracking ◽  
User Friendly ◽  
Track Analysis

Behavioral analysis of moving animals relies on a faithful recording and track analysis to extract relevant parameters of movement. To study group behavior and social interactions, often simultaneous analyses of individuals are required. To detect social interactions, for example to identify the leader of a group as opposed to followers, one needs an error-free segmentation of individual tracks throughout time. While automated tracking algorithms exist that are quick and easy to use, inevitable errors will occur during tracking. To solve this problem, we introduce a robust algorithm called epiTracker for segmentation and tracking of multiple animals in two-dimensional (2D) videos along with an easy-to-use correction method that allows one to obtain error-free segmentation. We have implemented two graphical user interfaces to allow user-friendly control of the functions. Using six labeled 2D datasets, the effort to obtain accurate labels is quantified and compared to alternative available software solutions. Both the labeled datasets and the software are publicly available.

Bilateral control system for laser micromanipulation by force feedback

2000 ◽  
Vol 14 (5) ◽  
pp. 381-383 ◽  
Author(s):  
Fumihito Arai ◽  
Masanobu Ogawa ◽  
Toshio Fukuda
Keyword(s):  
Control System ◽  
Force Feedback ◽  
Bilateral Control ◽  
Laser Micromanipulation
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