Measurements of picosecond lifetimes by time correlated single photon counting method: The effect of the refraction index of the solvent on the instrument response function

2004 ◽  
Vol 75 (10) ◽  
pp. 3107-3121 ◽  
Author(s):  
Teresa Wróżowa ◽  
Barbara Ciesielska ◽  
Dariusz Komar ◽  
Jerzy Karolczak ◽  
Andrzej Maciejewski ◽  
...  
Keyword(s):  
Response Function ◽  
Single Photon ◽  
Photon Counting ◽  
Refraction Index ◽  
Counting Method ◽  
Instrument Response Function ◽  
Single Photon Counting ◽  
Instrument Response ◽  
Photon Counting Method

scholarly journals On Synthetic Instrument Response Functions of Time-Correlated Single-Photon Counting Based Fluorescence Lifetime Imaging Analysis

2021 ◽  
Vol 9 ◽  
Author(s):  
Dong Xiao ◽  
Natakorn Sapermsap ◽  
Mohammed Safar ◽  
Margaret Rose Cunningham ◽  
Yu Chen ◽  
...  
Keyword(s):  
Response Function ◽  
Fluorescence Lifetime ◽  
Single Photon ◽  
Photon Counting ◽  
Simulated Data ◽  
Fluorescence Lifetime Imaging ◽  
Instrument Response Function ◽  
Single Photon Counting ◽  
Lifetime Imaging ◽  
Instrument Response

Time-correlated single-photon counting (TCSPC) has been the gold standard for fluorescence lifetime imaging (FLIM) techniques due to its high signal-to-noize ratio and high temporal resolution. The sensor system's temporal instrument response function (IRF) should be considered in the deconvolution procedure to extract the real fluorescence decay to compensate for the distortion on measured decays contributed by the system imperfections. However, to measure the instrument response function is not trivial, and the measurement setup is different from measuring the real fluorescence. On the other hand, automatic synthetic IRFs can be directly derived from the recorded decay profiles and provide appropriate accuracy. This paper proposed and examined a synthetic IRF strategy. Compared with traditional automatic synthetic IRFs, the new proposed automatic synthetic IRF shows a broader dynamic range and better accuracy. To evaluate its performance, we examined simulated data using nonlinear least square deconvolution based on both the Levenberg-Marquardt algorithm and the Laguerre expansion method for bi-exponential fluorescence decays. Furthermore, experimental FLIM data of cells were also analyzed using the proposed synthetic IRF. The results from both the simulated data and experimental FLIM data show that the proposed synthetic IRF has a better performance compared to traditional synthetic IRFs. Our work provides a faster and precise method to obtain IRF, which may find various FLIM-based applications. We also reported in which conditions a measured or a synthesized IRF can be applied.

Design Automation of Single Photon Counting Method for Quantum Random Number Generation

2021 ◽  
Author(s):  
Dwi Novazrianto ◽  
Alwan Muhammad Fajar ◽  
Aprilia Kusuma Dewi ◽  
Muhammad Yusuf ◽  
Rini Wisnu Wardhani ◽  
...  
Keyword(s):  
Design Automation ◽  
Random Number ◽  
Single Photon ◽  
Photon Counting ◽  
Random Number Generation ◽  
Counting Method ◽  
Single Photon Counting ◽  
Number Generation ◽  
Photon Counting Method

Measurement of fluorescence decay curves by the single photon counting method

1981 ◽  
Vol 34 (2) ◽  
pp. 165-168
Author(s):  
A. B. Evdokimov ◽  
G. V. Lukomskii ◽  
B. S. Neporent ◽  
O. V. Stolbova
Keyword(s):  
Single Photon ◽  
Photon Counting ◽  
Fluorescence Decay ◽  
Counting Method ◽  
Single Photon Counting ◽  
Photon Counting Method
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