Automation of High-Throughput Crystal Screening and Data Collection at SSRL

2004 ◽  
Author(s):  
Mitchell D. Miller
Keyword(s):  
Data Collection ◽  
High Throughput

scholarly journals Correction to: Soybean iron deficiency chlorosis high throughput phenotyping using an unmanned aircraft system

Plant Methods ◽  
2019 ◽  
Vol 15 (1) ◽  
Author(s):  
Austin A. Dobbels ◽  
Aaron J. Lorenz
Keyword(s):  
Iron Deficiency ◽  
Data Collection ◽  
Data Processing ◽  
High Throughput ◽  
Image Data ◽  
Unmanned Aircraft ◽  
Unmanned Aircraft System ◽  
Iron Deficiency Chlorosis ◽  
High Throughput Phenotyping ◽  
Aircraft System

In the original article [1], under the subheading “Image data processing”, last paragraph, last sentence that reads as “The least …… data collection” was incorrectly published. The correct sentence should read as “Least-significant differences (P < 0.20) were calculated for all 36 trials on both ground-based and UAS-image based scores for both dates of data collection.” The original article has been corrected.

scholarly journals NMRbot: Python scripts enable high-throughput data collection on current Bruker BioSpin NMR spectrometers

Metabolomics ◽  
2013 ◽  
Vol 9 (3) ◽  
pp. 558-563 ◽  
Author(s):  
Lawrence J. Clos ◽  
M. Fransisca Jofre ◽  
James J. Ellinger ◽  
William M. Westler ◽  
John L. Markley
Keyword(s):  
Data Collection ◽  
High Throughput ◽  
High Throughput Data

scholarly journals Robust indexing for automatic data collection

2004 ◽  
Vol 37 (3) ◽  
pp. 399-409 ◽  
Author(s):  
Nicholas K. Sauter ◽  
Ralf W. Grosse-Kunstleve ◽  
Paul D. Adams
Keyword(s):  
Data Collection ◽  
High Throughput ◽  
Visual Inspection ◽  
The Novel ◽  
Automatic Data ◽  
X Ray ◽  
Alternative Approach ◽  
Automatic Data Collection ◽  
Diffraction Patterns ◽  
Improved Methods

Improved methods for indexing diffraction patterns from macromolecular crystals are presented. The novel procedures include a more robust way to verify the position of the incident X-ray beam on the detector, an algorithm to verify that the deduced lattice basis is consistent with the observations, and an alternative approach to identify the metric symmetry of the lattice. These methods help to correct failures commonly experienced during indexing, and increase the overall success rate of the process. Rapid indexing, without the need for visual inspection, will play an important role as beamlines at synchrotron sources prepare for high-throughput automation.

scholarly journals High-throughput sample handling and data collection at synchrotrons: embedding the ESRF into the high-throughput gene-to-structure pipeline

2006 ◽  
Vol 62 (10) ◽  
pp. 1162-1169 ◽  
Author(s):  
A. Beteva ◽  
F. Cipriani ◽  
S. Cusack ◽  
S. Delageniere ◽  
J. Gabadinho ◽  
...  
Keyword(s):  
Data Collection ◽  
High Throughput ◽  
Sample Handling

scholarly journals MicroED data collection with SerialEM

2018 ◽  
Author(s):  
M. Jason de la Cruz ◽  
Michael W. Martynowycz ◽  
Johan Hattne ◽  
Tamir Gonen
Keyword(s):  
Data Collection ◽  
High Throughput ◽  
Large Scale ◽  
Large Scale Data ◽  
Data Analyses ◽  
Continuous Rotation ◽  
Scale Data

AbstractWe developed a procedure for the cryoEM method MicroED using SerialEM. With this approach, SerialEM coordinates stage rotation, microscope operation, and camera functions for automated continuous-rotation MicroED data collection. More than 300 datasets can be collected overnight in this way, facilitating high-throughput MicroED data collection for large-scale data analyses.

scholarly journals Case Study of High-Throughput Drug Screening and Remote Data Collection for SARS-CoV-2 Main Protease by Using Serial Femtosecond X-ray Crystallography

Crystals ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 1579
Author(s):  
Omur Guven ◽  
Mehmet Gul ◽  
Esra Ayan ◽  
J Austin Johnson ◽  
Baris Cakilkaya ◽  
...  
Keyword(s):  
Data Collection ◽  
High Throughput ◽  
Drug Screening ◽  
Dynamic Range ◽  
Drug Repurposing ◽  
Low Noise ◽  
Drug Binding ◽  
Array Detector ◽  
X Ray ◽  
Main Protease

Since early 2020, COVID-19 has grown to affect the lives of billions globally. A worldwide investigation has been ongoing for characterizing the virus and also for finding an effective drug and developing vaccines. As time has been of the essence, a crucial part of this research has been drug repurposing; therefore, confirmation of in silico drug screening studies have been carried out for this purpose. Here we demonstrated the possibility of screening a variety of drugs efficiently by leveraging a high data collection rate of 120 images/second with the new low-noise, high dynamic range ePix10k2M Pixel Array Detector installed at the Macromolecular Femtosecond Crystallography (MFX) instrument at the Linac Coherent Light Source (LCLS). The X-ray Free-Electron Laser (XFEL) is used for remote high-throughput data collection for drug repurposing of the main protease (Mpro) of SARS-CoV-2 at ambient temperature with mitigated X-ray radiation damage. We obtained multiple structures soaked with nine drug candidate molecules in two crystal forms. Although our drug binding attempts failed, we successfully established a high-throughput Serial Femtosecond X-ray crystallographic (SFX) data collection protocol.

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