Vacuum and Pressure System for NMR Sample Preparation

1963 ◽  
Vol 34 (8) ◽  
pp. 944-944 ◽  
Author(s):  
Robert A. Cacciarelli ◽  
Burch B. Stewart
Keyword(s):  
Sample Preparation ◽  
Pressure System ◽  
Nmr Sample Preparation

scholarly journals Electrokinetically Assisted Paper-Based DNA Concentration for Enhanced qPCR Sensing

Proceedings ◽  
2020 ◽  
Vol 60 (1) ◽  
pp. 33
Author(s):  
Md Nazibul Islam ◽  
Jarad Yost ◽  
Zachary Gagnon
Keyword(s):  
Sample Preparation ◽  
Nucleic Acids ◽  
Pore Size ◽  
Fluid Transport ◽  
Low Cost ◽  
Quantitative Polymerase Chain Reaction ◽  
Pressure System ◽  
Bulk Fluid ◽  
Paper Based Microfluidics ◽  
Copper Tape

Paper-based microfluidics have gained widespread attention for use as low-cost microfluidic diagnostic devices in low-resource settings. However, variability in fluid transport due to evaporation and lack of reproducibility with processing real-world samples limits their commercial potential and widespread adoption. We have developed a novel fabrication method to address these challenges. This approach, known as “Microfluidic Pressure in Paper” (μPiP), combines thin laminating polydimethylsiloxane (PDMS) membranes and precision laser-cut paper microfluidic structures to produce devices that are low-cost, scalable, and exhibit controllable and reproducible fluid flow dynamics similar to conventional microfluidic devices. We present a new μPiP DNA sample preparation and processing device that reduces the number of sample preparation steps and improves sensitivity of the quantitative polymerase chain reaction (qPCR) by electrophoretically separating and concentrating nucleic acids (NAs) continuously on paper. Our device was assembled using two different microfluidic paper channels: one with a larger pore (25 microns) size for bulk fluid transport and another with a smaller pore size (11 microns) for electrophoretic sample concentration. These two paper types were aligned and laminated within PDMS sheets, and integrated with adhesive copper tape electrodes. A solution containing a custom DNA sequence was introduced into the large pore size paper channel using a low-cost pressure system and a DC voltage was applied to the copper tape to electrophoretically deflect the solution containing NAs into the paper channel with the smaller pore size. Samples were collected from both DNA enriched and depleted channels and analyzed using qPCR. Our results demonstrate the ability to use these paper devices to process and concentrate nucleic acids. Our concentration device has the potential to reduce the number of sample preparation steps and to improve qPCR sensitivity, which has immediate applications in disease diagnostics, microbial contamination, and public health monitoring.

Submicro Scale NMR Sample Preparation for Volatile Chemicals

2004 ◽  
Vol 30 (11) ◽  
pp. 2153-2161 ◽  
Author(s):  
Satoshi Nojima ◽  
David J. Kiemle ◽  
Francis X. Webster ◽  
Wendell L. Roelofs
Keyword(s):  
Sample Preparation ◽  
Volatile Chemicals ◽  
Nmr Sample Preparation

A new solution 31 P NMR sample preparation scheme for marine sediments

2017 ◽  
Vol 15 (4) ◽  
pp. 381-393 ◽  
Author(s):  
Delphine Defforey ◽  
Barbara J. Cade-Menun ◽  
Adina Paytan
Keyword(s):  
Sample Preparation ◽  
Marine Sediments ◽  
Nmr Sample Preparation

scholarly journals A Triple Layer of Immiscible Solvents for NMR Sample Preparation: Enhanced Sensitivity and Reduced Deuterated Solvent for Observation of Heteronuclei

2019 ◽  
Author(s):  
Bikash Baishya ◽  
Atish Chandra
Keyword(s):  
Sample Preparation ◽  
Signal To Noise Ratio ◽  
Bottom Part ◽  
Enhanced Sensitivity ◽  
Magnetic Field Homogeneity ◽  
Field Homogeneity ◽  
Nmr Sample Preparation ◽  
Set Up ◽  
The Magnetic Field ◽  
Sample Dilution

<p>Organic chemistry labs routinely perform NMR in a standard 5 mm NMR tube. NMR sample is prepared by filling the lowermost 4 cm length of a regular 5 mm 0.d. tube that holds approximately 0.55 ml of a deuterated solvent. This is actually a sample dilution procedure as the signal mainly comes from the central part, i.e. 1.8 cm length sample that fits the typical coil length of 1.8 cm in regular NMR spectrometers. The diluted top and bottom part of the sample is away from the coil and contributes less signal. This dilution procedure amplifies the requirement of expensive deuterated solvent and lowers sensitivity. The present study explores a new way of sample preparation which involves sandwiching a small amount of the deuterated solvent (D<sub>2</sub>O) of length 1.8 cm containing the analyte between two non-deuterated solvents (CCl<sub>4</sub> at bottom of length ≈ 1 cm and similarly C<sub>6</sub>H<sub>6</sub> at top), which are immiscible with the former using a regular 5 mm o.d. NMR tube in such a way that total length is still 4 cm as demanded by the magnetic field homogeneity considerations. The analyte now being closer to NMR coil dissolved in 1.8 cm solvent, improves sensitivity and reduces deuterated solvent hitherto required. <sup>13</sup>C and <sup>15</sup>N spectra from such a set-up display two to three-fold higher signal to noise ratio and hence four to eightfold savings in experimental time or faster data collection.</p><br>

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