Local Compositional Changes in Alkali Silicate Glasses during Electron Microprobe Analysis

1967 ◽  
Vol 38 (5) ◽  
pp. 2406-2407 ◽  
Author(s):  
M. P. Borom ◽  
R. E. Hanneman
Keyword(s):  
Electron Microprobe ◽  
Microprobe Analysis ◽  
Electron Microprobe Analysis ◽  
Silicate Glasses ◽  
Alkali Silicate ◽  
Compositional Changes

Electron Microprobe Analysis of Weathered Florida Chert

1979 ◽  
Vol 44 (3) ◽  
pp. 517-524 ◽  
Author(s):  
David E. Clark ◽  
Barbara A. Purdy
Keyword(s):  
Electron Microprobe ◽  
Microprobe Analysis ◽  
Electron Microprobe Analysis ◽  
Sandy Clay ◽  
Diffusion Controlled ◽  
Stratigraphic Position ◽  
Acid Environment ◽  
Compositional Changes ◽  
Time Required ◽  
The Given

Underlying paleo-Indian period artifacts, deeply weathered chert implements have been recovered in Florida from a sandy clay horizon heretofore considered culturally sterile. Although the stratigraphic position of the stone implements in the sandy clay deposit has been demonstrated without question, dating has been a problem because no carbon-containing material has been preserved. Thus experiments were undertaken to investigate the environmental conditions under which the stone artifacts have weathered and the time required for the given extent of weathering. The electron microprobe was used to evaluate the surface compositional changes that occur in Florida chert during weathering. The patina of a chert surface exposed to an acid environment was found to be depleted of iron to a depth of ∼ 600μm. The mechanism through which Fe is leached from the surface is thought to be a diffusion-controlled process in which the depth of leaching is proportional to the square root of exposure time. This may provide a means of dating artifacts fashioned from these cherts.

Scanning Electron Microscopy and Electron Microprobe Analysis of Malignant Cell Cultures

1968 ◽  
Vol 26 ◽  
pp. 164-165
Author(s):  
R. I. Johnsson-Hegyeli ◽  
A. F. Hegyeli ◽  
D. K. Landstrom ◽  
W. C. Lane
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Cell Cultures ◽  
Electron Microprobe ◽  
Microprobe Analysis ◽  
Electron Microprobe Analysis ◽  
Three Dimensional ◽  
Malignant Cell ◽  
Interference Microscopy ◽  
Scanning Electron

Last year we reported on the use of reflected light interference microscopy (RLIM) for the direct color photography of the surfaces of living normal and malignant cell cultures without the use of replicas, fixatives, or stains. The surface topography of living cells was found to follow underlying cellular structures such as nuceloli, nuclear membranes, and cytoplasmic organelles, making possible the study of their three-dimensional relationships in time. The technique makes possible the direct examination of cells grown on opaque as well as transparent surfaces. The successful in situ electron microprobe analysis of the elemental composition and distribution within single tissue culture cells was also reported.This paper deals with the parallel and combined use of scanning electron microscopy (SEM) and the two previous techniques in a study of living and fixed cancer cells. All three studies can be carried out consecutively on the same experimental specimens without disturbing the cells or their structural relationships to each other and the surface on which they are grown. KB carcinoma cells were grown on glass coverslips in closed Leighto tubes as previously described. The cultures were photographed alive by means of RLIM, then fixed with a fixative modified from Sabatini, et al (1963).

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