Normal Vibrations of the Polymer Molecules of Helical Conformation. VI. Polytetrahydrofuran and Deuterated Polytetrahydrofurans

1965 ◽  
Vol 42 (8) ◽  
pp. 2807-2816 ◽  
Author(s):  
Kiyohisa Imada ◽  
Hiroyuki Tadokoro ◽  
Akira Umehara ◽  
Shunsuke Murahashi
Keyword(s):  
Helical Conformation ◽  
Normal Vibrations ◽  
Polymer Molecules

Normal Vibrations of the Polymer Molecules of Helical Conformation. V. Isotactic Polypropylene and Its Deuteroderivatives

1965 ◽  
Vol 42 (4) ◽  
pp. 1432-1449 ◽  
Author(s):  
Hiroyuki Tadokoro ◽  
Masamichi Kobayashi ◽  
Minoru Ukita ◽  
Katsutoshi Yasufuku ◽  
Shunsuke Murahashi ◽  
...  
Keyword(s):  
Isotactic Polypropylene ◽  
Helical Conformation ◽  
Normal Vibrations ◽  
Polymer Molecules

Normal Vibrations of the Polymer Molecules of Helical Configuration. III. Polyoxymethylene and Polyoxymethylene‐d2

1963 ◽  
Vol 38 (3) ◽  
pp. 703-721 ◽  
Author(s):  
Hiroyuki Tadokoro ◽  
Masamichi Kobayashi ◽  
Yôichi Kawaguchi ◽  
Akira Kobayashi ◽  
Shunsuke Murahashi
Keyword(s):  
Normal Vibrations ◽  
Polymer Molecules ◽  
Helical Configuration

scholarly journals Naphthalimide-Containing BP100 Leads to Higher Model Membranes Interactions and Antimicrobial Activity

Biomolecules ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 542
Author(s):  
Gustavo Penteado Battesini Carretero ◽  
Greice Kelle Viegas Saraiva ◽  
Magali Aparecida Rodrigues ◽  
Sumika Kiyota ◽  
Marcelo Porto Bemquerer ◽  
...  
Keyword(s):  
Anticancer Agents ◽  
Biological Activities ◽  
Biological Effects ◽  
Membrane Surface ◽  
Helical Structure ◽  
Helical Conformation ◽  
Property A ◽  
Cellular Processes ◽  
Double Stranded Dna ◽  
Low Toxicity

In a large variety of organisms, antimicrobial peptides (AMPs) are primary defenses against pathogens. BP100 (KKLFKKILKYL-NH2), a short, synthetic, cationic AMP, is active against bacteria and displays low toxicity towards eukaryotic cells. BP100 acquires a α-helical conformation upon interaction with membranes and increases membrane permeability. Despite the volume of information available, the action mechanism of BP100, the selectivity of its biological effects, and possible applications are far from consensual. Our group synthesized a fluorescent BP100 analogue containing naphthalimide linked to its N-terminal end, NAPHT-BP100 (Naphthalimide-AAKKLFKKILKYL-NH2). The fluorescence properties of naphthalimides, especially their spectral sensitivity to microenvironment changes, are well established, and their biological activities against transformed cells and bacteria are known. Naphthalimide derived compounds are known to interact with DNA disturbing related processes as replication and transcription, and used as anticancer agents due to this property. A wide variety of techniques were used to demonstrate that NAPHT-BP100 bound to and permeabilized zwitterionic POPC and negatively charged POPC:POPG liposomes and, upon interaction, acquired a α-helical structure. Membrane surface high peptide/lipid ratios triggered complete permeabilization of the liposomes in a detergent-like manner. Membrane disruption was driven by charge neutralization, lipid aggregation, and bilayer destabilization. NAPHT-BP100 also interacted with double-stranded DNA, indicating that this peptide could also affect other cellular processes besides causing membrane destabilization. NAPHT-BP100 showed increased antibacterial and hemolytic activities, compared to BP100, and may constitute an efficient antimicrobial agent for dermatological use. By conjugating BP100 and naphthalimide DNA binding properties, NAPHT-BP100 bound to a large extent to the bacterial membrane and could more efficiently destabilize it. We also speculate that peptide could enter the bacteria cell and interact with its DNA in the cytoplasm.

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