In Situ Electron‐Microscopic Observation of Epitaxial Growth of PbS and PbSe on MgO

1969 ◽  
Vol 40 (9) ◽  
pp. 3857-3859 ◽  
Author(s):  
K. Yagi ◽  
K. Kobayashi ◽  
G. Honjo
Keyword(s):  
Epitaxial Growth ◽  
Microscopic Observation ◽  
Electron Microscopic Observation ◽  
Electron Microscopic

scholarly journals Immunological dissimilarity in protein component (dynein 1) between outer and inner arms within sea urchin sperm axonemes

1982 ◽  
Vol 92 (3) ◽  
pp. 706-713 ◽  
Author(s):  
K Ogawa ◽  
S Negishi ◽  
M Obika
Keyword(s):  
Atpase Activity ◽  
Sea Urchin ◽  
Microscopic Observation ◽  
Electron Microscopic Observation ◽  
Double Diffusion ◽  
Diffusion Test ◽  
Electron Microscopic ◽  
Antibody Method ◽  
Sea Urchin Sperm

The 0.5 M KCl-treatment solubilizes the outer arms from sea urchin sperm axonemes. Approximately 30 percent of A-polypeptide, corresponding to dynein 1 in SDS- polyacrylamide gel, was solubilized by this treatment (as SEA-dynein 1). Electron microscopic observation indicated that the extracted axonemes lacked the outer arms in various degrees. The DEA-dynein 1 was that the extracted axonemes lacked the outer arms in various degrees. The SEA-dyenin 1 was purified and an antiserum against it was prepared in rabbits. The specificity of antiserum to dynein 1 was determined by immunoelectrophoresis and ouchterlony's double-diffusion test. The anti-dynein 1 serum inhibited ATPase activity of purified SEA-dynein 1 by 95 percent. By the indirect peroxidase-conjugated antibody method, the loci of SEA-dynein 1 within the intact, salt- extracted and mechanically disrupted axonemes were determined to be the outer arms: deposition of electron-dense materials which represents their localization was detected at the distal ends of the outer arms, in the case of intact axonemes. The 5-6 cross- bridge was hardly decorated. No decoration was seen in the salt-extracted axonemes lacking all the outer arms. In disrupted axonemes, which consist of single to several peripheral doublets, electron-dense materials were deposited only on the outer arms. Approximately 73 percent of axonemal ATPase activity sensitive to antiserum was solubilized by repeated salt-extractions. One-half of A-polypeptide (SEA-dynein 1 located at the outer arms) was contained in the pooled extracts. The extracted axonemes contained another half of A-polypeptide (SUA-dynein 1 supposed to locate at the inner arms) and retained 31 percent of axonemal ATPase activity that was almost resistant to antiserum. Solubilized SUA-dynein 1 was immunologically the same as SEA-dynein 1. This result indicates that in situ SUA-dynein 1 did not receive anti-dynein 1 antibodies, coinciding with the result obtained for salt-extracted axonemes lacking all the outer arms by the enzyme-antibody method mentioned above. These observations suggest that immunological dissimilarity in dynein 1 between outer and inner arms but do not tell us that the inner arms do not contain dynein 1.

scholarly journals In situ Electron Microscopic Observation of Negatively Stained Tissue Culture Cells Contaminated with Mycoplasmas

Microbiology ◽  
1983 ◽  
Vol 129 (11) ◽  
pp. 3379-3384
Author(s):  
H. Takayama ◽  
T. Katsumoto ◽  
K. Ohno ◽  
A. Nakaso ◽  
A. Takagi ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Microscopic Observation ◽  
Electron Microscopic Observation ◽  
Electron Microscopic ◽  
Culture Cells ◽  
Tissue Culture Cells

In situ electron microscopic observation of electrochemical Li-intercalation into MoS2

2020 ◽  
Vol 357 ◽  
pp. 115488
Author(s):  
Kaname Yoshida ◽  
Tadahiro Kawasaki ◽  
Akihide Kuwabara ◽  
Yoshio Ukyo ◽  
Yuichi Ikuhara
Keyword(s):  
Microscopic Observation ◽  
Electron Microscopic Observation ◽  
Electron Microscopic

In Situ Liquid Cell Transmission Electron Microscopic Observation of Electron Beam Induced Au Crystal Growth in a Solution

2012 ◽  
Vol 18 (S2) ◽  
pp. 1098-1099 ◽  
Author(s):  
J. Park ◽  
S. Kodambaka ◽  
F.M. Ross ◽  
J.M. Grogan ◽  
H.H. Bau
Keyword(s):  
Electron Beam ◽  
Crystal Growth ◽  
Microscopic Observation ◽  
Electron Microscopic Observation ◽  
Electron Microscopic ◽  
Transmission Electron ◽  
Transmission Electron Microscopic Observation ◽  
Transmission Electron Microscopic ◽  
Liquid Cell

Extended abstract of a paper presented at Microscopy and Microanalysis 2012 in Phoenix, Arizona, USA, July 29 – August 2, 2012.

Electron Microscopic Observation of the Longitudinal Organization of Poly (p-Phenylene Terephthalamide) Fibers

1982 ◽  
Vol 40 ◽  
pp. 680-681
Author(s):  
Li Li-Sheng ◽  
L.F. Allard ◽  
W.C. Bigelow
Keyword(s):  
Electron Microscopy ◽  
Mechanical Properties ◽  
Scanning Electron Microscopy ◽  
Microscopic Observation ◽  
Electron Microscopic Observation ◽  
Electron Microscopic ◽  
Aromatic Polyamides ◽  
Radial Arrangement ◽  
Scanning Electron ◽  
Better Than

The aromatic polyamides form a class of fibers having mechanical properties which are much better than those of aliphatic polyamides. Currently, the accepted morphology of these fibers as proposed by M.G. Dobb, et al. is a radial arrangement of pleated sheets, with the plane of the pleats parallel to the axis of the fiber. We have recently obtained evidence which supports a different morphology of this type of fiber, using ultramicrotomy and ion-thinning techniques to prepare specimens for transmission and scanning electron microscopy.

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