OBSERVATION OF SPONTANEOUS MODE LOCKING IN A HIGH‐PRESSURE CO2LASER

1970 ◽  
Vol 17 (11) ◽  
pp. 474-476 ◽  
Author(s):  
D. L. Lyon ◽  
E. V. George ◽  
H. A. Haus
Keyword(s):  
High Pressure ◽  
Mode Locking

PASSIVE MODE LOCKING OF A HIGH-PRESSURE CO2LASER WITH A CO2SATURABLE ABSORBER

1971 ◽  
Vol 18 (12) ◽  
pp. 546-548 ◽  
Author(s):  
A. F. Gibson ◽  
M. F. Kimmitt ◽  
C. A. Rosito
Keyword(s):  
High Pressure ◽  
Mode Locking ◽  
Passive Mode

Active mode locking of a high-pressure pulsed N2O laser

1971 ◽  
Vol 7 (9) ◽  
pp. 462-463 ◽  
Author(s):  
J. Gilbert ◽  
J. Lachambre ◽  
F. Rheault
Keyword(s):  
High Pressure ◽  
Mode Locking ◽  
Active Mode ◽  
N2o Laser

Active mode locking of a high pressure CW waveguide CO2 laser

1987 ◽  
Vol 44 (2) ◽  
pp. 107-110 ◽  
Author(s):  
G. L. Bourdet ◽  
R. A. Muller ◽  
G. M. Mullot ◽  
J. Y. Vinet
Keyword(s):  
High Pressure ◽  
Co2 Laser ◽  
Mode Locking ◽  
Active Mode

The Forced Mode-Locking of a Transversely Excited High Pressure CO2Laser

1973 ◽  
Vol 12 (12) ◽  
pp. 1950-1951 ◽  
Author(s):  
Toshio Sakane ◽  
Hiroshi Kashiwagi
Keyword(s):  
High Pressure ◽  
Mode Locking

High-pressure freezing of cells in suspension for low-voltage scanning electron microscopy (LVSEM)

1991 ◽  
Vol 49 ◽  
pp. 64-65
Author(s):  
Marek Malecki ◽  
James Pawley ◽  
Hans Ris
Keyword(s):  
Electron Microscopy ◽  
High Pressure ◽  
Low Voltage ◽  
Culture Media ◽  
Cell Structure ◽  
Freeze Substitution ◽  
Ice Crystal ◽  
High Pressure Freezing ◽  
Cell Culture Media ◽  
Voltage Scanning

The ultrastructure of cells suspended in physiological fluids or cell culture media can only be studied if the living processes are stopped while the cells remain in suspension. Attachment of living cells to carrier surfaces to facilitate further processing for electron microscopy produces a rapid reorganization of cell structure eradicating most traces of the structures present when the cells were in suspension. The structure of cells in suspension can be immobilized by either chemical fixation or, much faster, by rapid freezing (cryo-immobilization). The fixation speed is particularly important in studies of cell surface reorganization over time. High pressure freezing provides conditions where specimens up to 500μm thick can be frozen in milliseconds without ice crystal damage. This volume is sufficient for cells to remain in suspension until frozen. However, special procedures are needed to assure that the unattached cells are not lost during subsequent processing for LVSEM or HVEM using freeze-substitution or freeze drying. We recently developed such a procedure.

Prolonged Fixation Studies For Spaceflight

1973 ◽  
Vol 31 ◽  
pp. 332-333
Author(s):  
Robert Corbett ◽  
Delbert E. Philpott ◽  
Sam Black
Keyword(s):  
High Pressure ◽  
Living Systems ◽  
Prolonged Storage ◽  
Time Intervals ◽  
Early Signs ◽  
Large Numbers

Observation of subtle or early signs of change in spaceflight induced alterations on living systems require precise methods of sampling. In-flight analysis would be preferable but constraints of time, equipment, personnel and cost dictate the necessity for prolonged storage before retrieval. Because of this, various tissues have been stored in fixatives and combinations of fixatives and observed at various time intervals. High pressure and the effect of buffer alone have also been tried.Of the various tissues embedded, muscle, cartilage and liver, liver has been the most extensively studied because it contains large numbers of organelles common to all tissues (Fig. 1).

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