scholarly journals Rotational dynamics of colloidal spheres probed with fluorescence recovery after photobleaching

2004 ◽  
Vol 120 (9) ◽  
pp. 4517-4529 ◽  
Author(s):  
M. P. Lettinga ◽  
G. H. Koenderink ◽  
B. W. M. Kuipers ◽  
E. Bessels ◽  
A. P. Philipse
Keyword(s):  
Fluorescence Recovery After Photobleaching ◽  
Rotational Dynamics ◽  
Fluorescence Recovery ◽  
Colloidal Spheres

scholarly journals Improved Model of Fluorescence Recovery Expands the Application of Multiphoton Fluorescence Recovery after Photobleaching in Vivo

2009 ◽  
Vol 96 (12) ◽  
pp. 5082-5094 ◽  
Author(s):  
Kelley D. Sullivan ◽  
William H. Sipprell ◽  
Edward B. Brown ◽  
Edward B. Brown
Keyword(s):  
Fluorescence Recovery After Photobleaching ◽  
Fluorescence Recovery ◽  
Improved Model

scholarly journals Role for Actin in the Polarized Release of Rotavirus

2007 ◽  
Vol 81 (9) ◽  
pp. 4892-4894 ◽  
Author(s):  
Agnès Gardet ◽  
Michelyne Breton ◽  
Germain Trugnan ◽  
Serge Chwetzoff
Keyword(s):  
Fluorescence Recovery After Photobleaching ◽  
Spike Protein ◽  
Fluorescence Recovery ◽  
Actin Network ◽  
Apical Pole ◽  
Apical Side ◽  
Functional Consequences ◽  
Diffusional Mobility

ABSTRACT Rotaviruses are characterized by polarized release from the apical side of infected enterocytes, and the rotavirus VP4 spike protein specifically binds to the actin network at the apical pole of differentiated enterocytic cells. To determine the functional consequences of this VP4-actin interaction, fluorescence recovery after photobleaching experiments were carried out to measure the diffusional mobility of VP4 associated with the microfilaments. Results show that VP4 binds to barbed ends of microfilaments by using actin treadmilling. Actin treadmilling inhibition results in the loss of rotavirus apical preferential release, suggesting a major role for actin in polarized rotavirus release.

A novel mesoionic carbene based highly fluorescent Pd(ii) complex as an endoplasmic reticulum tracker in live cells

2018 ◽  
Vol 47 (44) ◽  
pp. 15646-15650 ◽  
Author(s):  
Sanjay K. Verma ◽  
Pratibha Kumari ◽  
Shagufi Naz Ansari ◽  
Mohd Ovais Ansari ◽  
Dondinath Deori ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Fluorescence Recovery After Photobleaching ◽  
Fluorescence Recovery ◽  
Live Cells

Synthesis of new organometallic MIC based mononuclear Pd(ii) complex 1, specifically target ER of live cells and have fluorescence recovery after photobleaching (FRAP) property.

Fluorescence recovery after photobleaching reveals that LPS rapidly transfers from CD14 to hsp70 and hsp90 on the cell membrane

2001 ◽  
Vol 114 (13) ◽  
pp. 2535-2545
Author(s):  
Kathy Triantafilou ◽  
Martha Triantafilou ◽  
Shab Ladha ◽  
Alan Mackie ◽  
Russell L. Dedrick ◽  
...  
Keyword(s):  
Fluorescence Recovery After Photobleaching ◽  
Lipoteichoic Acid ◽  
Fluorescence Recovery ◽  
Outer Cell ◽  
Immune Recognition ◽  
Gram Positive Bacteria ◽  
Innate Recognition ◽  
Functional Receptor ◽  
Attachment Process ◽  
Outer Cell Wall

Although CD14 has been implicated in the immune recognition of bacterial lipopolysaccharide (LPS) from Gram-negative bacteria and also peptidoglycan (PGN) and lipoteichoic acid (LTA) from the outer cell wall of Gram-positive bacteria, accumulating evidence has suggested the possible existence of other functional receptor(s). In this study, we have used fluorescence recovery after photobleaching (FRAP) in order to get the first dynamic picture of the innate recognition of bacteria. We have found that the diffusion coefficient of CD14 remains unaffected after LPS ligation and that the diffusion coefficients of FITC-LPS and FITC-LTA bound to cells differ from that of CD14. Furthermore, FITC-LPS/LTA rapidly become immobile when bound to cells, suggesting that FITC-LPS/LTA must briefly associate with CD14 in the initial attachment process and rapidly move on to an immobile receptor or to a complex of receptors. Further FRAP experiments revealed that heat shock protein 70 (hsp70) and hsp90 are immobile in cell membranes, and antibodies against them were found to block the transfer of LPS to the immobile receptor and to inhibit interleukin 6 production upon LPS stimulation. These experiments indicated that LPS transfers from CD14 to hsp70 and hsp90, which may be part of an LPS/LTA multimeric receptor complex. Thus, hsps are implicated as mediators of the innate activation by bacteria.

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