Dynamics of the spontaneous formation of a planar phospholipid bilayer: A new approach by simultaneous electrical and optical measurements

2003 ◽  
Vol 119 (13) ◽  
pp. 6768-6775 ◽  
Author(s):  
Hisashi Fujiwara ◽  
Masayuki Fujihara ◽  
Takashi Ishiwata
1979 ◽  
Author(s):  
L. Mester ◽  
L. Szabados ◽  
J.M. Launay ◽  
M. Mester

I-Desoxy-l-(5-hydroxytryptamino)-D-fructose is a synthetic sugar derivative of serotonin, however, its spontaneous formation under physiological conditions is quite possible (5th Inter. Congr. Thromb. Haemost., Paris, 1975, Abstr. n°233). This, and similar sugar derivatives of serotonin show a serotonin-like activity, however, a discrepancy has been observed between their affinities towards D and M type tryptaminergic receptors, when compared with the affinity of serotonin for the same receptors. The sugar derivatives have been distinguished by an increased passage of the blood-brain barrier and by a limited uptake by theplatelet. They exhibit a mechanism which mildly inhibits platelet functions, while mildly supporting haemostasis by vascular smooth muscle contraction. Besides the regulation of blood pressure, heart rate and sinusal coronary flow, some typical central effects (anti-stress activity, suppression of the carotid occlusion response, etc.) have also been observed, which have a beneficial effect on the cardiovascular system. Progressing in this way, it is hoped to have a new approach to inhibiting thrombosis without significantly interfering with haemostasis.


2002 ◽  
Vol 3 (1) ◽  
pp. 27-35 ◽  
Author(s):  
C. A. Naumann ◽  
O. Prucker ◽  
T. Lehmann ◽  
J. Rühe ◽  
W. Knoll ◽  
...  

2015 ◽  
Vol 135 ◽  
pp. 274-285 ◽  
Author(s):  
J. De Gracia ◽  
A. Boyano ◽  
A. Arrese ◽  
F. Mujika

1999 ◽  
Author(s):  
Vadim P. Veiko ◽  
Nikolay B. Voznesensky ◽  
Vitaly M. Domnenko ◽  
Alexey E. Goussev ◽  
Tatyana V. Ivanova ◽  
...  

2020 ◽  
Vol 18 (1) ◽  
pp. 97-112 ◽  
Author(s):  
Vyacheslav I. Terkulov

The paper is devoted to compound abbreviated appellatives, i.e. non-initial common abbreviations, related to the collocations by means of “spontaneous” formation (in diachrony) and/or motivation (in synchrony).The relevance of the investigation is determined by implementing a new approach to interpreting abbreviation based on identifying onomasiologically significant mechanisms of the producing unit transformation into an abbreviation as well as on the differentiating synchronous and diachronic methods to abbreviations. As the result, the author can define new principles of abbreviations’ lexicographic description. The aim of the article is to establish the basic options for determining and describing compound abbreviated appellatives as an autonomous onomasiological type of abbreviations in diachrony and in synchrony. The novelty of the research consists in the following facts: firstly, the necessity to differentiate abbreviations as nominative units, arising from overcoming asymmetric dualism of descriptive collocations, from reductions, which act as formal doublets of abbreviated words because they preserve the onomasiological status of the original unit, is justified; secondly, it defines the reasons to refer compound abbreviated appellatives to an autonomous onomasiological class of abbreviations; thirdly, it shows the need to differentiate synchronous and diachronic descriptions of abbreviations. The practical significance lies in the fact that the results of the study can be used for composing dictionaries of abbreviations.


1999 ◽  
Vol 173 ◽  
pp. 185-188
Author(s):  
Gy. Szabó ◽  
K. Sárneczky ◽  
L.L. Kiss

AbstractA widely used tool in studying quasi-monoperiodic processes is the O–C diagram. This paper deals with the application of this diagram in minor planet studies. The main difference between our approach and the classical O–C diagram is that we transform the epoch (=time) dependence into the geocentric longitude domain. We outline a rotation modelling using this modified O–C and illustrate the abilities with detailed error analysis. The primary assumption, that the monotonity and the shape of this diagram is (almost) independent of the geometry of the asteroids is discussed and tested. The monotonity enables an unambiguous distinction between the prograde and retrograde rotation, thus the four-fold (or in some cases the two-fold) ambiguities can be avoided. This turned out to be the main advantage of the O–C examination. As an extension to the theoretical work, we present some preliminary results on 1727 Mette based on new CCD observations.


Author(s):  
V. Mizuhira ◽  
Y. Futaesaku

Previously we reported that tannic acid is a very effective fixative for proteins including polypeptides. Especially, in the cross section of microtubules, thirteen submits in A-tubule and eleven in B-tubule could be observed very clearly. An elastic fiber could be demonstrated very clearly, as an electron opaque, homogeneous fiber. However, tannic acid did not penetrate into the deep portion of the tissue-block. So we tried Catechin. This shows almost the same chemical natures as that of proteins, as tannic acid. Moreover, we thought that catechin should have two active-reaction sites, one is phenol,and the other is catechole. Catechole site should react with osmium, to make Os- black. Phenol-site should react with peroxidase existing perhydroxide.


Author(s):  
K. Chien ◽  
R. Van de Velde ◽  
I.P. Shintaku ◽  
A.F. Sassoon

Immunoelectron microscopy of neoplastic lymphoma cells is valuable for precise localization of surface antigens and identification of cell types. We have developed a new approach in which the immunohistochemical staining can be evaluated prior to embedding for EM and desired area subsequently selected for ultrathin sectioning.A freshly prepared lymphoma cell suspension is spun onto polylysine hydrobromide- coated glass slides by cytocentrifugation and immediately fixed without air drying in polylysine paraformaldehyde (PLP) fixative. After rinsing in PBS, slides are stained by a 3-step immunoperoxidase method. Cell monolayer is then fixed in buffered 3% glutaraldehyde prior to DAB reaction. After the DAB reaction step, wet monolayers can be examined under LM for presence of brown reaction product and selected monolayers then processed by routine methods for EM and embedded with the Chien Re-embedding Mold. After the polymerization, the epoxy blocks are easily separated from the glass slides by heatingon a 100°C hot plate for 20 seconds.


Author(s):  
W. E. Lee

An optical waveguide consists of a several-micron wide channel with a slightly different index of refraction than the host substrate; light can be trapped in the channel by total internal reflection.Optical waveguides can be formed from single-crystal LiNbO3 using the proton exhange technique. In this technique, polished specimens are masked with polycrystal1ine chromium in such a way as to leave 3-13 μm wide channels. These are held in benzoic acid at 249°C for 5 minutes allowing protons to exchange for lithium ions within the channels causing an increase in the refractive index of the channel and creating the waveguide. Unfortunately, optical measurements often reveal a loss in waveguiding ability up to several weeks after exchange.


Author(s):  
W. A. Chiou ◽  
N. Kohyama ◽  
B. Little ◽  
P. Wagner ◽  
M. Meshii

The corrosion of copper and copper alloys in a marine environment is of great concern because of their widespread use in heat exchangers and steam condensers in which natural seawater is the coolant. It has become increasingly evident that microorganisms play an important role in the corrosion of a number of metals and alloys under a variety of environments. For the past 15 years the use of SEM has proven to be useful in studying biofilms and spatial relationships between bacteria and localized corrosion of metals. Little information, however, has been obtained using TEM capitalizing on its higher spacial resolution and the transmission observation of interfaces. The research presented herein is the first step of this new approach in studying the corrosion with biological influence in pure copper.Commercially produced copper (Cu, 99%) foils of approximately 120 μm thick exposed to a copper-tolerant marine bacterium, Oceanospirillum, and an abiotic culture medium were subsampled (1 cm × 1 cm) for this study along with unexposed control samples.


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