Three-dimensional orientation of single molecules observed by far- and near-field fluorescence microscopy

2003 ◽  
Vol 118 (12) ◽  
pp. 5279-5282 ◽  
Author(s):  
Martin Vacha ◽  
Masahiro Kotani
Keyword(s):  
Fluorescence Microscopy ◽  
Near Field ◽  
Single Molecules ◽  
Three Dimensional

Two-Photon Microscopy of Single Molecules

2000 ◽  
Vol 6 (S2) ◽  
pp. 802-803
Author(s):  
J. T. Fourkas ◽  
M. J. R. Previte ◽  
R. A. Farrer ◽  
C. Olson ◽  
L. A. Peyser
Keyword(s):  
Near Field ◽  
Single Molecules ◽  
Three Dimensional ◽  
Excitation Beam ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Higher Power ◽  
Excitation Techniques ◽  
Field Excitation ◽  
Heterogeneous Ensemble

The ability to observe the fluorescence arising from single molecules has revolutionized our ability to study the structure and dynamics of materials on a microscopic level and to probe the properties of individual members of a heterogeneous ensemble. A variety of near-field and far-field excitation techniques have been employed to study single molecules. Multiphoton excitation (MPE) techniques have a number of advantages that make them particularly attractive for singlemolecule detection. First, because the excitation and fluorescence wavelengths are significantly different from one another, Rayleigh and Raman scattering can easily be filtered out, leading to a low number of background counts. Second, because the probability for MPE depends on the excitation intensity to the second or higher power, the excitation is localized to the point in space where the excitation beam is most tightly focussed, thus providing three-dimensional resolution.

scholarly journals Simultaneous near-field and far-field fluorescence microscopy of single molecules

2011 ◽  
Vol 19 (7) ◽  
pp. 6836 ◽  
Author(s):  
Thomas Ruckstuhl ◽  
Dorinel Verdes ◽  
Christian M. Winterflood ◽  
Stefan Seeger
Keyword(s):  
Fluorescence Microscopy ◽  
Near Field ◽  
Single Molecules ◽  
Far Field

Nanocrystals and nano-optics

1995 ◽  
Vol 353 (1703) ◽  
pp. 313-321 ◽  
Keyword(s):  
Optical Spectroscopy ◽  
Quantum Confinement ◽  
Near Field ◽  
Science And Technology ◽  
Semiconductor Nanocrystals ◽  
Single Molecules ◽  
Three Dimensional ◽  
Nanometer Scale ◽  
New Science

Three dimensional electronic quantum confinement in semiconductor nanocrystals, and near-field optical spectroscopy of single molecules, are briefly discussed as examples of new science and technology at the nanometer scale.

scholarly journals Imaging of bi-anisotropic periodic structures from electromagnetic near-field data

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Dinh-Liem Nguyen ◽  
Trung Truong
Keyword(s):  
Inverse Scattering ◽  
Maxwell Equations ◽  
Field Data ◽  
Periodic Structures ◽  
Near Field ◽  
Scattering Problem ◽  
Three Dimensional ◽  
Inverse Scattering Problem ◽  
Factorization Method ◽  
Unique Determination

AbstractThis paper is concerned with the inverse scattering problem for the three-dimensional Maxwell equations in bi-anisotropic periodic structures. The inverse scattering problem aims to determine the shape of bi-anisotropic periodic scatterers from electromagnetic near-field data at a fixed frequency. The factorization method is studied as an analytical and numerical tool for solving the inverse problem. We provide a rigorous justification of the factorization method which results in the unique determination and a fast imaging algorithm for the periodic scatterer. Numerical examples for imaging three-dimensional periodic structures are presented to examine the efficiency of the method.

Three-Dimensional Finite-Difference Time-Domain Method Modeling of Nanowire Optical Probe

2014 ◽  
Vol 602-605 ◽  
pp. 3359-3362
Author(s):  
Chun Li Zhu ◽  
Jing Li
Keyword(s):  
Finite Difference ◽  
Time Domain ◽  
Finite Difference Time Domain ◽  
Incident Light ◽  
Near Field ◽  
Three Dimensional ◽  
Polarization Direction ◽  
Near Field Imaging ◽  
Difference Time ◽  
Field Imaging

In this paper, output near fields of nanowires with different optical and structure configurations are calculated by using the three-dimensional finite-difference time-domain (3D FDTD) method. Then a nanowire with suitable near field distribution is chosen as the probe for scanning dielectric and metal nanogratings. Scanning results show that the resolution in near-field imaging of dielectric nanogratings can be as low as 80nm, and the imaging results are greatly influenced by the polarization direction of the incident light. Compared with dielectric nanogratings, metal nanogratings have significantly enhanced resolutions when the arrangement of gratings is perpendicular to the polarization direction of the incident light due to the enhancement effect of the localized surface plasmons (SPs). Results presented here could offer valuable references for practical applications in near-field imaging with nanowires as optical probes.

scholarly journals Automated Three-Dimensional Detection and Shape Classification of Dendritic Spines from Fluorescence Microscopy Images

PLoS ONE ◽  
2008 ◽  
Vol 3 (4) ◽  
pp. e1997 ◽  
Author(s):  
Alfredo Rodriguez ◽  
Douglas B. Ehlenberger ◽  
Dara L. Dickstein ◽  
Patrick R. Hof ◽  
Susan L. Wearne
Keyword(s):  
Fluorescence Microscopy ◽  
Dendritic Spines ◽  
Three Dimensional ◽  
Shape Classification ◽  
Microscopy Images
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