Single-molecule near-field optical energy transfer microscopy

2002 ◽  
Vol 81 (11) ◽  
pp. 2118-2120 ◽  
Author(s):  
W. Trabesinger ◽  
A. Kramer ◽  
M. Kreiter ◽  
B. Hecht ◽  
U. P. Wild
Keyword(s):  
Energy Transfer ◽  
Single Molecule ◽  
Near Field ◽  
Optical Energy

Single-molecule near-field optical energy transfer microscopy with dielectric tips

2003 ◽  
Vol 209 (3) ◽  
pp. 249-253 ◽  
Author(s):  
W. Trabesinger ◽  
A. Kramer ◽  
M. Kreiter ◽  
B. Hecht ◽  
U. P. Wild
Keyword(s):  
Energy Transfer ◽  
Single Molecule ◽  
Near Field ◽  
Optical Energy

Single molecule fluorescence resonance energy transfer scanning near-field optical microscopy: potentials and challenges

2015 ◽  
Vol 184 ◽  
pp. 51-69 ◽  
Author(s):  
S. K. Sekatskii ◽  
K. Dukenbayev ◽  
M. Mensi ◽  
A. G. Mikhaylov ◽  
E. Rostova ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Fluorescence Resonance Energy Transfer ◽  
Single Molecule ◽  
Near Field ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Nitrogen Vacancy ◽  
Single Molecule Fluorescence ◽  
Single Molecule Fret ◽  
Fluorescence Resonance

A few years ago, single molecule Fluorescence Resonance Energy Transfer Scanning Near-Field Optical Microscope (FRET SNOM) images were demonstrated using CdSe semiconductor nanocrystal–dye molecules as donor–acceptor pairs. Corresponding experiments reveal the necessity to exploit much more photostable fluorescent centers for such an imaging technique to become a practically used tool. Here we report the results of our experiments attempting to use nitrogen vacancy (NV) color centers in nanodiamond (ND) crystals, which are claimed to be extremely photostable, for FRET SNOM. All attempts were unsuccessful, and as a plausible explanation we propose the absence (instability) of NV centers lying close enough to the ND border. We also report improvements in SNOM construction that are necessary for single molecule FRET SNOM imaging. In particular, we present the first topographical images of single strand DNA molecules obtained with fiber-based SNOM. The prospects of using rare earth ions in crystals, which are known to be extremely photostable, for single molecule FRET SNOM at room temperature and quantum informatics at liquid helium temperatures, where FRET is a coherent process, are also discussed.

Heat-assisted magnetic recording by a near-field transducer with efficient optical energy transfer

2009 ◽  
Vol 3 (4) ◽  
pp. 220-224 ◽  
Author(s):  
W. A. Challener ◽  
Chubing Peng ◽  
A. V. Itagi ◽  
D. Karns ◽  
Wei Peng ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Magnetic Recording ◽  
Near Field ◽  
Optical Energy ◽  
Heat Assisted Magnetic Recording

scholarly journals Erratum: Heat-assisted magnetic recording by a near-field transducer with efficient optical energy transfer

2009 ◽  
Vol 3 (5) ◽  
pp. 303-303 ◽  
Author(s):  
W. A. Challener ◽  
Chubing Peng ◽  
A. V. Itagi ◽  
D. Karns ◽  
Wei Peng ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Magnetic Recording ◽  
Near Field ◽  
Optical Energy ◽  
Heat Assisted Magnetic Recording

Monitoring the Structural Dynamics of U2 snRNA Via Single-Molecule Förster Resonance Energy Transfer

2018 ◽  
Author(s):  
Alexander Carl DeHaven
Keyword(s):  
Energy Transfer ◽  
Structural Dynamics ◽  
Single Molecule ◽  
Conformational Dynamics ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Förster Resonance Energy Transfer ◽  
U2 Snrna ◽  
Folding Dynamics ◽  
The Impact

This thesis contains four topic areas: a review of single-molecule microscropy methods and splicing, conformational dynamics of stem II of the U2 snRNA, the impact of post-transcriptional modifications on U2 snRNA folding dynamics, and preliminary findings on Mango aptamer folding dynamics.

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