A high sensitivity electron momentum spectrometer with simultaneous detection in energy and momentum

2002 ◽  
Vol 73 (6) ◽  
pp. 2242-2248 ◽  
Author(s):  
Masahiko Takahashi ◽  
Taku Saito ◽  
Motoaki Matsuo ◽  
Yasuo Udagawa
Keyword(s):  
Simultaneous Detection ◽  
High Sensitivity ◽  
Electron Momentum ◽  
Energy And Momentum

scholarly journals Microfluidics-Based Plasmonic Biosensing System Based on Patterned Plasmonic Nanostructure Arrays

Micromachines ◽  
2021 ◽  
Vol 12 (7) ◽  
pp. 826
Author(s):  
Yanting Liu ◽  
Xuming Zhang
Keyword(s):  
Plasmon Resonance ◽  
Point Of Care ◽  
Simultaneous Detection ◽  
High Sensitivity ◽  
Microfluidic Chips ◽  
Label Free ◽  
Plasmonic Nanostructure ◽  
Point Of Care Diagnostics ◽  
Surface Enhanced Raman ◽  
Nanostructure Arrays

This review aims to summarize the recent advances and progress of plasmonic biosensors based on patterned plasmonic nanostructure arrays that are integrated with microfluidic chips for various biomedical detection applications. The plasmonic biosensors have made rapid progress in miniaturization sensors with greatly enhanced performance through the continuous advances in plasmon resonance techniques such as surface plasmon resonance (SPR) and localized SPR (LSPR)-based refractive index sensing, SPR imaging (SPRi), and surface-enhanced Raman scattering (SERS). Meanwhile, microfluidic integration promotes multiplexing opportunities for the plasmonic biosensors in the simultaneous detection of multiple analytes. Particularly, different types of microfluidic-integrated plasmonic biosensor systems based on versatile patterned plasmonic nanostructured arrays were reviewed comprehensively, including their methods and relevant typical works. The microfluidics-based plasmonic biosensors provide a high-throughput platform for the biochemical molecular analysis with the advantages such as ultra-high sensitivity, label-free, and real time performance; thus, they continue to benefit the existing and emerging applications of biomedical studies, chemical analyses, and point-of-care diagnostics.

scholarly journals Simultaneous Rapid Detection of Aflatoxin B1 and Ochratoxin A in Spices Using Lateral Flow Immuno-Chromatographic Assay

Foods ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2738
Author(s):  
Xue Zhao ◽  
Xindi Jin ◽  
Zhang Lin ◽  
Qi Guo ◽  
Bin Liu ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Aflatoxin B1 ◽  
High Performance ◽  
Simultaneous Detection ◽  
High Sensitivity ◽  
Strong Stability ◽  
Test Strip ◽  
Chromatography Analysis ◽  
Relative Standard ◽  
Strip Test

Spices are susceptible to contamination by aflatoxin B1 (AFB1) and ochratoxin A (OTA), which are both mycotoxins with high toxicity and carcinogenicity. In this study, we aimed to develop an immuno-chromatographic strip test for the simultaneous quantification of AFB1 and OTA in spices by spraying the coupled antigens AFB1–ovalbumin (AFB1–OVA) and OTA–ovalbumin (OTA–OVA) on a nitrocellulose membrane. The test strip had high sensitivity, good specificity, and strong stability. The detection limits of these two mycotoxins in Chinese prickly ash, pepper, chili, cinnamon, and aniseed were 5 μg/kg. The false positivity rate was 2%, and the false negativity rate was 0%. The maximum coefficient of variation was 4.28% between batches and 5.72% within batches. The average recovery rates of AFB1 and OTA in spices were 81.2–113.7% and 82.2–118.6%, respectively, and the relative standard deviation (RSD) was <10%. The actual sample detection was consistent with high performance liquid chromatography analysis results. Therefore, the immuno-chromatographic test strips developed in this study can be used for the on-site simultaneous detection of AFB1 and OTA in spices. This method would allow the relevant regulatory agencies to strengthen supervision in an effort to reduce the possible human health hazards of such contaminated spices.

Simultaneous sensitive analysis of Cd(ii), Pb(ii) and As(iii) using a dual-channel anodic stripping voltammetry approach

2020 ◽  
Vol 44 (15) ◽  
pp. 5739-5745
Author(s):  
Lijuan Bu ◽  
Qingji Xie ◽  
Hai Ming
Keyword(s):  
Heavy Metals ◽  
Anodic Stripping Voltammetry ◽  
Simultaneous Detection ◽  
High Sensitivity ◽  
Stripping Voltammetry ◽  
Sensitive Analysis ◽  
Trace Heavy Metals ◽  
Dual Channel ◽  
Anodic Stripping

A dual-channel anodic stripping voltammetry protocol is proposed for the simultaneous detection of trace heavy metals in one experiment with high sensitivity.

A high-sensitivity angle and energy dipersive multichannel electron momentum spectrometer with 2πangle range

2011 ◽  
Vol 82 (3) ◽  
pp. 033110 ◽  
Author(s):  
QiGuo Tian ◽  
KeDong Wang ◽  
Xu Shan ◽  
XiangJun Chen
Keyword(s):  
High Sensitivity ◽  
Electron Momentum

scholarly journals Recent Advances in the Detection of Neurotransmitters

Chemosensors ◽  
2018 ◽  
Vol 6 (1) ◽  
pp. 1 ◽  
Author(s):  
Bo Si ◽  
Edward Song
Keyword(s):  
Ex Vivo ◽  
Simultaneous Detection ◽  
High Sensitivity ◽  
Mental Illnesses ◽  
Electrochemical Sensing ◽  
Fast Scan Cyclic Voltammetry ◽  
In Vivo Detection ◽  
Recent Developments ◽  
Materials Used

Neurotransmitters are chemicals that act as messengers in the synaptic transmission process. They are essential for human health and any imbalance in their activities can cause serious mental disorders such as Parkinson’s disease, schizophrenia, and Alzheimer’s disease. Hence, monitoring the concentrations of various neurotransmitters is of great importance in studying and diagnosing such mental illnesses. Recently, many researchers have explored the use of unique materials for developing biosensors for both in vivo and ex vivo neurotransmitter detection. A combination of nanomaterials, polymers, and biomolecules were incorporated to implement such sensor devices. For in vivo detection, electrochemical sensing has been commonly applied, with fast-scan cyclic voltammetry being the most promising technique to date, due to the advantages such as easy miniaturization, simple device architecture, and high sensitivity. However, the main challenges for in vivo electrochemical neurotransmitter sensors are limited target selectivity, large background signal and noise, and device fouling and degradation over time. Therefore, achieving simultaneous detection of multiple neurotransmitters in real time with long-term stability remains the focus of research. The purpose of this review paper is to summarize the recently developed sensing techniques with the focus on neurotransmitters as the target analyte, and to discuss the outlook of simultaneous detection of multiple neurotransmitter species. This paper is organized as follows: firstly, the common materials used for developing neurotransmitter sensors are discussed. Secondly, several sensor surface modification approaches to enhance sensing performance are reviewed. Finally, we discuss recent developments in the simultaneous detection capability of multiple neurotransmitters.

scholarly journals Tools for diagnosis, monitoring and screening ofSchistosomainfections utilizing lateral-flow based assays and upconverting phosphor labels

Parasitology ◽  
2014 ◽  
Vol 141 (14) ◽  
pp. 1841-1855 ◽  
Author(s):  
PAUL L. A. M. CORSTJENS ◽  
CLAUDIA J. DE DOOD ◽  
DIEUWKE KORNELIS ◽  
ELISA M. TJON KON FAT ◽  
R. ALAN WILSON ◽  
...  
Keyword(s):  
Full Range ◽  
Simultaneous Detection ◽  
High Sensitivity ◽  
Low Complexity ◽  
Lateral Flow ◽  
Antibody Detection ◽  
Multiple Test ◽  
Control Programmes ◽  
Recent Developments ◽  
Single Worm

SUMMARYThe potential of various quantitative lateral flow (LF) based assays utilizing up-converting phosphor (UCP) reporters for the diagnosis of schistosomiasis is reviewed including recent developments. Active infections are demonstrated by screening for the presence of regurgitated worm antigens (genus specific polysaccharides), whereas anti-Schistosomaantibodies may indicate ongoing as well as past infections. The circulating anodic antigen (CAA) in serum or urine (and potentially also saliva) is identified as the marker that may allow detection of single-worm infections. Quantitation of antigen levels is a reliable method to study effects of drug administration, worm burden and anti-fecundity mechanisms. Moreover, the ratio of CAA and circulating cathodic antigen (CCA) is postulated to facilitate identification of eitherSchistosoma mansoniorSchistosoma haematobiuminfections. The UCP-LF assays allow simultaneous detection of multiple targets on a single strip, a valuable feature for antibody detection assays. Although antibody detection in endemic regions is not a useful tool to diagnose active infections, it gains potential when the ratio of different classes of antibody specific for the parasite/disease can be determined. The UCP-LF antibody assay format allows this type of multiplexing, including testing a linear array of up to 20 different targets. Multiple test spots would allow detection of specific antibodies, e.g. against differentSchistosomaspecies or other pathogens as soil-transmitted helminths. Concluding, the different UCP-LF based assays for diagnosis of schistosomiasis provide a collection of tests with relatively low complexity and high sensitivity, covering the full range of diagnostics needed in control programmes for mapping, screening and monitoring.

scholarly journals Comparison of Different Double Immunostaining Protocols for Paraffin Embedded Liver Tissue

1999 ◽  
Vol 18 (4) ◽  
pp. 227-233 ◽  
Author(s):  
Alexander Schütz ◽  
Andrea Tannapfel ◽  
Christian Wittekind
Keyword(s):  
Alkaline Phosphatase ◽  
Liver Tissue ◽  
Simultaneous Detection ◽  
High Sensitivity ◽  
Time Cost ◽  
Double Immunostaining ◽  
Intracellular Protein ◽  
Background Staining ◽  
Fresh Frozen ◽  
Sensitivity Specificity

Most of the double immunostaining protocols that have been introduced so far have been developed for application on fresh frozen material or based on different species antibodies. In liver tissue, general problems of double immunostaining techniques are further complicated by tissue‐specific difficulties, such as necrosis or high intracellular protein content. To assess a reliable double immunostaining protocol for archived, paraffin embedded liver tissue, different protocols based on the use of same species primary antibodies were evaluated in terms of sensitivity, specificity and non‐specific background staining in pathological liver specimens. We compared peroxidase–anti‐peroxidase, alkaline phosphatase–anti‐alkaline phosphatase (PAP/APAP), labelled‐avidin–biotin (LAB/LAB) and digoxigenin–anti‐digoxigenin (dig–a‐dig/PAP) techniques using different cytokeratin antibodies and an antibody against PCNA. Comparison of the double immunostaining techniques revealed a high sensitivity and specificity in all procedures. Sections, which were stained employing PAP/APAP‐technique, displayed a higher background staining compared to sections which were treated with the LAB/LAB or dig–a‐dig/PAP protocol. In contrast to the dig–a‐dig/PAP protocol, the LAB/LAB technique provides a better time/cost relationship. Therefore, we would like to recommend a modified LAB/LAB protocol for simultaneous detection of different antigens in archived liver tissue.

scholarly journals Nucleic acid-based detection of influenza A virus subtypes H7 and N9 with a special emphasis on the avian H7N9 virus

2014 ◽  
Vol 19 (10) ◽  
Author(s):  
D Kalthoff ◽  
J Bogs ◽  
T Harder ◽  
C Grund ◽  
A Pohlmann ◽  
...  
Keyword(s):  
Influenza A Virus ◽  
Influenza A ◽  
Animal Experiments ◽  
Simultaneous Detection ◽  
High Sensitivity ◽  
Epidemiological Studies ◽  
Severe Illness ◽  
The Novel ◽  
H7n9 Virus ◽  
Diagnostic Approaches

In 2013, a novel influenza A virus of subtype H7N9 was transmitted from avian sources to humans in China, causing severe illness and substantial mortality. Rapid and sensitive diagnostic approaches are the basis of epidemiological studies and of utmost importance for the detection of infected humans and animals. We developed various quantitative reverse transcriptase PCR (RT-qPCR) assays for (i) the generic detection of the haemagglutinin (HA) gene of H7 viruses or the neuraminidase (NA) gene of N9 viruses, and (ii) the specific detection of HA and NA of the novel avian H7N9/2013 virus. The sensitivity of the newly developed assays was compared with previously published PCRs, and the specificity of all RT-qPCRs was examined using a panel of 42 different H7 and 16 different N9 isolates. Furthermore, we analysed the performance of the RT-qPCR assays with dilution series and diagnostic samples obtained from animal experiments. Our study provides a comprehensive set of RT-qPCR assays for the reliable detection of the novel avian H7N9 virus, with high sensitivity and improved and tailored specificity values compared with published assays. Finally, we also present data about the robustness of a duplex assay for the simultaneous detection of HA and NA of the avian influenza H7N9/2013 virus.

scholarly journals Bioprocessing and integration of a high flux screening systematic platform based on isothermal amplification for the detection on 8 common pathogens

2020 ◽  
Author(s):  
Huamin Zhong ◽  
Hongwei Deng ◽  
Ming Li ◽  
Huahong Zhong
Keyword(s):  
Simultaneous Detection ◽  
High Sensitivity ◽  
Isothermal Amplification ◽  
High Flux ◽  
Double Blind ◽  
Screening Platform ◽  
Unique Specificity ◽  
Species Specific ◽  
Sensitivity Specificity ◽  
Human Infections

Abstract During a large variety of common pathogens, E. coli, P. aeruginosa, MRSA, MRCNS, V. parahaemolyticus, L. monocytogenes and Salmonella are the leading pathogens responsible for large number of human infections and diseases. In this study, a high flux screening based on nucleic acid isothermal amplification technique has been developed. For the 8 common pathogens, species-specific targets had been selected and analyzed for their unique specificity. After optimization, separate LAMP reaction assays had been bioprocessed and integrated into one systematic detection platform, including 8 strips (PCR tubes) and 96-well plates. Eight standard strains verified for the accuracy. Application of the established high flux screening platform was used for detection for 48 samples in 4 different 96-well plates, with 2 groups of 2 operators using double-blind procedure. The accuracy of 100% was obtained, with the total time consumption as 66–75 min (for 12 samples detection on 8 different pathogens). As concluded, through the bioprocess of the systematic platform based on LAMP technique, it’s been demonstrated to be capable of simultaneous detection of 8 pathogens, with high sensitivity, specificity, rapidity and convenience.

Sign in / Sign up

Export Citation Format

Share Document