Slice imaging: A new approach to ion imaging and velocity mapping

2001 ◽  
Vol 72 (10) ◽  
pp. 3848-3853 ◽  
Author(s):  
Christoph R. Gebhardt ◽  
T. Peter Rakitzis ◽  
Peter C. Samartzis ◽  
Vlassis Ladopoulos ◽  
Theofanis N. Kitsopoulos
Keyword(s):  
Ion Imaging ◽  
Velocity Mapping ◽  
New Approach ◽  
Slice Imaging

Multiphoton ionization/dissociation dynamics of formyl fluoride by velocity mapping ion imaging

2009 ◽  
Vol 11 (39) ◽  
pp. 8733 ◽  
Author(s):  
Fengyan Wang ◽  
Yongwei Zhang ◽  
Hua Wang ◽  
Jie Liu ◽  
Bo Jiang ◽  
...  
Keyword(s):  
Multiphoton Ionization ◽  
Ion Imaging ◽  
Velocity Mapping ◽  
Dissociation Dynamics

Visualizing rotational wave functions of electronically excited nitric oxide molecules by using an ion imaging technique

2018 ◽  
Vol 20 (5) ◽  
pp. 3303-3309 ◽  
Author(s):  
Kenta Mizuse ◽  
Nao Chizuwa ◽  
Dai Ikeda ◽  
Takashi Imajo ◽  
Yasuhiro Ohshima
Keyword(s):  
Nitric Oxide ◽  
Imaging Technique ◽  
Wave Functions ◽  
Ion Imaging ◽  
Rotational Wave ◽  
Electronically Excited ◽  
Slice Imaging

Rotational eigenstates in electronically excited NO molecules have been visualized by a photoion spatial-slice imaging technique.

Slice imaging and velocity mapping using a single field

2006 ◽  
Vol 77 (8) ◽  
pp. 083101 ◽  
Author(s):  
Vassilios Papadakis ◽  
Theofanis N. Kitsopoulos
Keyword(s):  
Velocity Mapping ◽  
Single Field ◽  
Slice Imaging

Photodissociation dynamics of bromoiodomethane from the first and second absorption bands. A combined velocity map and slice imaging study

2018 ◽  
Vol 20 (5) ◽  
pp. 3490-3503 ◽  
Author(s):  
Sonia Marggi Poullain ◽  
David V. Chicharro ◽  
Eduardo Navarro ◽  
Luis Rubio-Lago ◽  
Jesús González-Vázquez ◽  
...  
Keyword(s):  
Bond Cleavage ◽  
Imaging Study ◽  
Ion Imaging ◽  
Absorption Bands ◽  
Slice Imaging

Ion imaging is applied to disentangle the selective bond cleavage in the photodissociation of bromoiodomethane from the two first absorption bands.

Dark-Field Scanning Transmission Ion Microscopy via Detection of Forward-Scattered Helium Ions with a Microchannel Plate

2016 ◽  
Vol 22 (3) ◽  
pp. 544-550 ◽  
Author(s):  
Taylor J. Woehl ◽  
Ryan M. White ◽  
Robert R. Keller
Keyword(s):  
Magnetite Nanoparticles ◽  
Dark Field ◽  
Microchannel Plate ◽  
Sensitive Detector ◽  
Ion Imaging ◽  
Helium Ions ◽  
New Approach ◽  
Scanning Transmission ◽  
Atomic Number Contrast ◽  
Ion Microscopy

AbstractA microchannel plate was used as an ion sensitive detector in a commercial helium ion microscope (HIM) for dark-field transmission imaging of nanomaterials, i.e. scanning transmission ion microscopy (STIM). In contrast to previous transmission HIM approaches that used secondary electron conversion holders, our new approach detects forward-scattered helium ions on a dedicated annular shaped ion sensitive detector. Minimum collection angles between 125 mrad and 325 mrad were obtained by varying the distance of the sample from the microchannel plate detector during imaging. Monte Carlo simulations were used to predict detector angular ranges at which dark-field images with atomic number contrast could be obtained. We demonstrate atomic number contrast imagingviascanning transmission ion imaging of silica-coated gold nanoparticles and magnetite nanoparticles. Although the resolution of STIM is known to be degraded by beam broadening in the substrate, we imaged magnetite nanoparticles with high contrast on a relatively thick silicon nitride substrate. We expect this new approach to annular dark-field STIM will open avenues for more quantitative ion imaging techniques and advance fundamental understanding of underlying ion scattering mechanisms leading to image formation.

The O–C diagrams of minor planets − a new approach to modelling the rotation

1999 ◽  
Vol 173 ◽  
pp. 185-188
Author(s):  
Gy. Szabó ◽  
K. Sárneczky ◽  
L.L. Kiss
Keyword(s):  
Error Analysis ◽  
Time Dependence ◽  
Theoretical Work ◽  
Minor Planet ◽  
Minor Planets ◽  
New Approach ◽  
Preliminary Results ◽  
Ccd Observations

AbstractA widely used tool in studying quasi-monoperiodic processes is the O–C diagram. This paper deals with the application of this diagram in minor planet studies. The main difference between our approach and the classical O–C diagram is that we transform the epoch (=time) dependence into the geocentric longitude domain. We outline a rotation modelling using this modified O–C and illustrate the abilities with detailed error analysis. The primary assumption, that the monotonity and the shape of this diagram is (almost) independent of the geometry of the asteroids is discussed and tested. The monotonity enables an unambiguous distinction between the prograde and retrograde rotation, thus the four-fold (or in some cases the two-fold) ambiguities can be avoided. This turned out to be the main advantage of the O–C examination. As an extension to the theoretical work, we present some preliminary results on 1727 Mette based on new CCD observations.

A New Approach to the Demonstration of Oxidase-Localization Using Tannic Acid Or Catechin

1973 ◽  
Vol 31 ◽  
pp. 698-699
Author(s):  
V. Mizuhira ◽  
Y. Futaesaku
Keyword(s):  
Cross Section ◽  
Tannic Acid ◽  
Elastic Fiber ◽  
The Other ◽  
New Approach ◽  
Tissue Block ◽  
Active Reaction ◽  
The Cross

Previously we reported that tannic acid is a very effective fixative for proteins including polypeptides. Especially, in the cross section of microtubules, thirteen submits in A-tubule and eleven in B-tubule could be observed very clearly. An elastic fiber could be demonstrated very clearly, as an electron opaque, homogeneous fiber. However, tannic acid did not penetrate into the deep portion of the tissue-block. So we tried Catechin. This shows almost the same chemical natures as that of proteins, as tannic acid. Moreover, we thought that catechin should have two active-reaction sites, one is phenol,and the other is catechole. Catechole site should react with osmium, to make Os- black. Phenol-site should react with peroxidase existing perhydroxide.

Ratio imaging of intracellular ion concentration

1992 ◽  
Vol 50 (2) ◽  
pp. 1160-1161
Author(s):  
Stephen R. Bolsover
Keyword(s):  
Individual Cell ◽  
Video Camera ◽  
Field Of View ◽  
Ion Concentration ◽  
Fluorescence Signal ◽  
Ion Imaging ◽  
Ratiometric Fluorescence ◽  
Ratio Imaging ◽  
The Mean ◽  
The Many

The field of intracellular ion concentration measurement expanded greatly in the 1980's due primarily to the development by Roger Tsien of ratiometric fluorescence dyes. These dyes have many applications, and in particular they make possible to image ion concentrations: to produce maps of the ion concentration within living cells. Ion imagers comprise a fluorescence microscope, an imaging light detector such as a video camera, and a computer system to process the fluorescence signal and display the map of ion concentration.Ion imaging can be used for two distinct purposes. In the first, the imager looks at a field of cells, measuring the mean ion concentration in each cell of the many in the field of view. One can then, for instance, challenge the cells with an agonist and examine the response of each individual cell. Ion imagers are not necessary for this sort of experiment: one can instead use a system that measures the mean ion concentration in a just one cell at any one time. However, they are very much more convenient.

Processing Immunoperoxidase Labeled Cell Monolayers and Cryostat Sesctions For Electron Microscopy

1985 ◽  
Vol 43 ◽  
pp. 714-715
Author(s):  
K. Chien ◽  
R. Van de Velde ◽  
I.P. Shintaku ◽  
A.F. Sassoon
Keyword(s):  
Cell Monolayer ◽  
Cell Types ◽  
Surface Antigens ◽  
Immunoperoxidase Method ◽  
Reaction Step ◽  
Hot Plate ◽  
Air Drying ◽  
New Approach ◽  
Cell Monolayers ◽  
Glass Slides

Immunoelectron microscopy of neoplastic lymphoma cells is valuable for precise localization of surface antigens and identification of cell types. We have developed a new approach in which the immunohistochemical staining can be evaluated prior to embedding for EM and desired area subsequently selected for ultrathin sectioning.A freshly prepared lymphoma cell suspension is spun onto polylysine hydrobromide- coated glass slides by cytocentrifugation and immediately fixed without air drying in polylysine paraformaldehyde (PLP) fixative. After rinsing in PBS, slides are stained by a 3-step immunoperoxidase method. Cell monolayer is then fixed in buffered 3% glutaraldehyde prior to DAB reaction. After the DAB reaction step, wet monolayers can be examined under LM for presence of brown reaction product and selected monolayers then processed by routine methods for EM and embedded with the Chien Re-embedding Mold. After the polymerization, the epoxy blocks are easily separated from the glass slides by heatingon a 100°C hot plate for 20 seconds.

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