scholarly journals Effect of Recombinant Surfactant Protein C–Based Surfactant on the Acute Respiratory Distress Syndrome

2004 ◽  
Vol 351 (9) ◽  
pp. 884-892 ◽  
Author(s):  
Roger G. Spragg ◽  
James F. Lewis ◽  
Hans-Dieter Walmrath ◽  
Jay Johannigman ◽  
Geoff Bellingan ◽  
...  
Keyword(s):  
Acute Respiratory Distress Syndrome ◽  
Respiratory Distress Syndrome ◽  
Respiratory Distress ◽  
Protein C ◽  
Distress Syndrome ◽  
Surfactant Protein ◽  
Surfactant Protein C

scholarly journals Synthetic surfactant with a recombinant surfactant protein C analogue improves lung function and attenuates inflammation in a model of acute respiratory distress syndrome in adult rabbits

2019 ◽  
Vol 20 (1) ◽  
Author(s):  
J. Zebialowicz Ahlström ◽  
F. Massaro ◽  
P. Mikolka ◽  
R. Feinstein ◽  
G. Perchiazzi ◽  
...  
Keyword(s):  
Body Weight ◽  
Acute Respiratory Distress Syndrome ◽  
Lung Function ◽  
Respiratory Distress Syndrome ◽  
Respiratory Distress ◽  
Protein C ◽  
Distress Syndrome ◽  
Surfactant Protein ◽  
Lung Oedema ◽  
Surfactant Protein C

Abstract Aim In acute respiratory distress syndrome (ARDS) damaged alveolar epithelium, leakage of plasma proteins into the alveolar space and inactivation of pulmonary surfactant lead to respiratory dysfunction. Lung function could potentially be restored with exogenous surfactant therapy, but clinical trials have so far been disappointing. These negative results may be explained by inactivation and/or too low doses of the administered surfactant. Surfactant based on a recombinant surfactant protein C analogue (rSP-C33Leu) is easy to produce and in this study we compared its effects on lung function and inflammation with a commercial surfactant preparation in an adult rabbit model of ARDS. Methods ARDS was induced in adult New Zealand rabbits by mild lung-lavages followed by injurious ventilation (VT 20 m/kg body weight) until P/F ratio < 26.7 kPa. The animals were treated with two intratracheal boluses of 2.5 mL/kg of 2% rSP-C33Leu in DPPC/egg PC/POPG, 50:40:10 or poractant alfa (Curosurf®), both surfactants containing 80 mg phospholipids/mL, or air as control. The animals were subsequently ventilated (VT 8–9 m/kg body weight) for an additional 3 h and lung function parameters were recorded. Histological appearance of the lungs, degree of lung oedema and levels of the cytokines TNFα IL-6 and IL-8 in lung homogenates were evaluated. Results Both surfactant preparations improved lung function vs. the control group and also reduced inflammation scores, production of pro-inflammatory cytokines, and formation of lung oedema to similar degrees. Poractant alfa improved compliance at 1 h, P/F ratio and PaO2 at 1.5 h compared to rSP-C33Leu surfactant. Conclusion This study indicates that treatment of experimental ARDS with synthetic lung surfactant based on rSP-C33Leu improves lung function and attenuates inflammation.

Treatment of Acute Respiratory Distress Syndrome with Recombinant Surfactant Protein C Surfactant

2003 ◽  
Vol 167 (11) ◽  
pp. 1562-1566 ◽  
Author(s):  
Roger G. Spragg ◽  
James F. Lewis ◽  
Wilhelm Wurst ◽  
Dietrich Häfner ◽  
Robert P. Baughman ◽  
...  
Keyword(s):  
Acute Respiratory Distress Syndrome ◽  
Respiratory Distress Syndrome ◽  
Respiratory Distress ◽  
Protein C ◽  
Distress Syndrome ◽  
Surfactant Protein ◽  
Surfactant Protein C

scholarly journals Very low surfactant protein C contents in newborn Belgian White and Blue calves with respiratory distress syndrome

2000 ◽  
Vol 351 (3) ◽  
pp. 779-787 ◽  
Author(s):  
Fabien DANLOIS ◽  
Shahparak ZALTASH ◽  
Jan JOHANSSON ◽  
Bengt ROBERTSON ◽  
Henk P. HAAGSMAN ◽  
...  
Keyword(s):  
Surface Tension ◽  
Respiratory Distress Syndrome ◽  
Respiratory Distress ◽  
Protein C ◽  
Distress Syndrome ◽  
Surfactant Protein ◽  
Healthy Controls ◽  
Surfactant Protein C ◽  
Organic Extracts ◽  
Newborn Calves

We have studied a respiratory distress syndrome (RDS) occurring in newborn calves of the Belgian White and Blue (BWB) breed that represents the large majority of beef cattle in Belgium. Pulmonary surfactant isolated from 14 BWB newborn calves that died from RDS and from 7 healthy controls was analysed for composition and surface activity. An extremely low content or, in some instances, an absence of surfactant protein C (SP-C) was detected in the RDS samples by Western blotting and differential amino acid analysis [0.03±0.01% (w/w) relative to total phospholipids, compared with 0.39±0.06% for healthy controls (means±S.E.M., P < 0.001)]. The contents of surfactant protein B (SP-B) were similar in RDS and control samples. The crude surfactant samples isolated from RDS calves had higher ratios of total protein to total phospholipid, altered phospholipid profiles and lower SP-A contents. Both crude and organic extracts of RDS surfactant samples showed increased dynamic surface tension compared with healthy controls when evaluated with a pulsating-bubble surfactometer. The addition of purified SP-C to organic extracts of RDS surfactant samples lowered surface tension. Strongly decreased levels of mature SP-C associated with fatal RDS and altered surface activity in vitro have, to the best of our knowledge, not been previously reported. The mechanisms underlying RDS and the decrease in SP-C in BWB calves remain to be established.

scholarly journals Two Mutations in Surfactant Protein C Gene Associated with Neonatal Respiratory Distress

2015 ◽  
Vol 2015 ◽  
pp. 1-3 ◽  
Author(s):  
Anna Tarocco ◽  
Elisa Ballardini ◽  
Maria Raffaella Contiero ◽  
Giampaolo Garani ◽  
Silvia Fanaro
Keyword(s):  
Respiratory Distress Syndrome ◽  
Respiratory Distress ◽  
Protein C ◽  
Distress Syndrome ◽  
Clinical Manifestations ◽  
Male Infant ◽  
Surfactant Protein ◽  
Extremely Low Birth Weight ◽  
Surfactant Protein C ◽  
Neonatal Respiratory Distress

Multiple mutations of surfactant genes causing surfactant dysfunction have been described. Surfactant protein C (SP-C) deficiency is associated with variable clinical manifestations ranging from neonatal respiratory distress syndrome to lethal lung disease. We present an extremely low birth weight male infant with an unusual course of respiratory distress syndrome associated with two mutations in the SFTPC gene: C43-7G>A and 12T>A. He required mechanical ventilation for 26 days and was treated with 5 subsequent doses of surfactant with temporary and short-term efficacy. He was discharged at 37 weeks of postconceptional age without any respiratory support. During the first 16 months of life he developed five respiratory infections that did not require hospitalization.Conclusion. This mild course in our patient with two mutations is peculiar because the outcome in patients with a single SFTPC mutation is usually poor.

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