Regulation of Human MC2-R Gene Expression by CREB, CREM, and ICER in the Adrenocortical Cell Line Y1

2007 ◽  
Vol 39 (8) ◽  
pp. 560-566 ◽  
Author(s):  
O. Zwermann ◽  
A. Braun ◽  
E. Lalli ◽  
P. Sassone-Corsi ◽  
F. Beuschlein ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
R Gene ◽  
Adrenocortical Cell

Regulation of ACTH-R gene expression by CREB, CREMt and ICER in the adrenocortical cell line Y1

2005 ◽  
Vol 113 (S 1) ◽  
Author(s):  
O Zwermann ◽  
A Braun ◽  
E Lalli ◽  
F Beuschlein ◽  
M Reincke
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
R Gene ◽  
Adrenocortical Cell

scholarly journals Inhibition of cellular proliferation by the Wilms' tumor suppressor WT1 is associated with suppression of insulin-like growth factor I receptor gene expression.

1995 ◽  
Vol 15 (7) ◽  
pp. 3516-3522 ◽  
Author(s):  
H Werner ◽  
Z Shen-Orr ◽  
F J Rauscher ◽  
J F Morris ◽  
C T Roberts ◽  
...  
Keyword(s):  
Gene Expression ◽  
Growth Factor ◽  
Tumor Suppressor ◽  
Cell Line ◽  
Cellular Proliferation ◽  
Wilms Tumor ◽  
Gene Promoter ◽  
R Gene ◽  
Factor I ◽  
Igf I

We have investigated the regulation of the insulin-like growth factor I receptor (IGF-I-R) gene promoter by the Wilms' tumor suppressor WT1 in intact cells. The levels of endogenous IGF-I-R mRNA and the activity of IGF-I-R gene promoter fragments in luciferase reporter constructs were found to be significantly higher in G401 cells (a Wilms' tumor-derived cell line lacking detectable WT1 mRNA) than in 293 cells (a human embryonic kidney cell line which expresses significant levels of WT1 mRNA). To study whether WT1 could suppress the expression of the endogenous IGF-I-R gene, WT1-negative G401 cells were stably transfected with a WT1 expression vector. Expression of WT1 mRNA in G401 cells resulted in a significant decrease in the rate of cellular proliferation, which was associated with a reduction in the levels of IGF-I-R mRNA, promoter activity, and ligand binding and with a reduction in IGF-I-stimulated cellular proliferation, thymidine incorporation, and anchorage-independent growth. These data suggest that a major aspect of the action of the WT1 tumor suppressor is the repression of IGF-I-R gene expression.

scholarly journals Adrenal Corticosteroid Perturbation by the Endocrine Disruptor BDE-47 in a Human Adrenocortical Cell Line and Male Rats

Endocrinology ◽  
2021 ◽  
Author(s):  
Benjamin M Dungar ◽  
Chad D Schupbach ◽  
Jessie R Jacobson ◽  
Phillip G Kopf
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
Polybrominated Diphenyl Ethers ◽  
Plasma Corticosterone ◽  
Male Rats ◽  
Cortisol Secretion ◽  
Adrenocortical Cell ◽  
Sprague Dawley ◽  
Treatment Groups ◽  
Corticosteroid Secretion

Abstract Polybrominated diphenyl ethers (PBDEs) have been previously shown to alter various endocrine biosynthetic pathways. Growing epidemiological evidence suggests that PBDEs alter cardiovascular function. The goal of this study was to examine the effects of BDE-47 on adrenal corticosteroid pathways that play vital roles in cardiovascular homeostasis and pathophysiology. The effect of BDE-47 on aldosterone and cortisol secretion was characterized in a human adrenocortical cell line. HAC15 cells were exposed to various concentrations of BDE-47 (1 nM-100 μM). Cell viability, corticosteroid secretion, gene expression of enzymes involved in corticosteroid synthesis, and metabolic activity was examined. Additionally, Sprague Dawley male rats were orally exposed to BDE-47 (10 or 100 µg/kg), 5 days per week for 16 weeks. Organ weights and plasma corticosteroid levels were measured. In HAC15 cells, basal and stimulated aldosterone and cortisol secretion was significantly increased by BDE-47. Gene expression of several enzymes involved in corticosteroid synthesis and mitochondrial metabolism were also increased. In Sprague Dawley rats, adrenal, but not heart, kidney, or liver weights, were significantly increased in BDE-47 treatment groups. Plasma corticosterone levels were significantly increased in the 100 µg BDE-47/kg treatment group. No change in plasma aldosterone levels were observed with BDE-47 exposure. These data indicate that BDE-47 disrupts the regulation of corticosteroid secretion and provides further evidence that PBDEs are potential endocrine disruptors. Future studies will determine the underlying molecular mechanism of altered corticosteroid production and examine whether these alterations result in underlying cardiovascular disease in our rodent model of 16 week BDE-47 exposure.

Effects of mitotane on gene expression in the adrenocortical cell line NCI-H295R: a microarray study

2012 ◽  
Vol 13 (12) ◽  
pp. 1351-1361 ◽  
Author(s):  
Adrienn Zsippai ◽  
Diana Rita Szabó ◽  
Zsófia Tömböl ◽  
Peter M Szabó ◽  
Katalin Éder ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
Adrenocortical Cell ◽  
Microarray Study
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