Optimal Cell Source for Cardiovascular Tissue Engineering: Venous vs. Aortic Human Myofibroblasts

2001 ◽  
Vol 49 (04) ◽  
pp. 221-225 ◽  
Author(s):  
A. Schnell ◽  
S. Hoerstrup ◽  
G. Zund ◽  
S. Kolb ◽  
R. Sodian ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cardiovascular Tissue ◽  
Cell Source ◽  
Cardiovascular Tissue Engineering

Cryopreserved prenatal progenitor cells as cell source for cardiovascular tissue engineering

2007 ◽  
Vol 55 (S 1) ◽  
Author(s):  
D Schmidt ◽  
C Breymann ◽  
J Achermann ◽  
B Odermatt ◽  
M Genoni ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Progenitor Cells ◽  
Cardiovascular Tissue ◽  
Cell Source ◽  
Cardiovascular Tissue Engineering

Human Adipose Stem Cells: A Potential Cell Source for Cardiovascular Tissue Engineering

2008 ◽  
Vol 187 (4) ◽  
pp. 263-274 ◽  
Author(s):  
Sepideh Heydarkhan-Hagvall ◽  
Katja Schenke-Layland ◽  
Jin Q. Yang ◽  
Sanaz Heydarkhan ◽  
Yuhuan Xu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Adipose Stem Cells ◽  
Cardiovascular Tissue ◽  
Cell Source ◽  
Cardiovascular Tissue Engineering ◽  
Human Adipose Stem Cells

The cardiotomy reservoir – a preliminary evaluation of a new cell source for cardiovascular tissue engineering

2017 ◽  
Vol 41 (2) ◽  
pp. 115-123
Author(s):  
Sophie von Nathusius ◽  
Fabian König ◽  
Ralf Sodian ◽  
Frank Born ◽  
Christian Hagl ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Lines ◽  
Easy Access ◽  
Control Group ◽  
Positive Staining ◽  
Widespread Application ◽  
Blood Samples ◽  
Cardiovascular Tissue ◽  
Cell Source ◽  
Cardiovascular Tissue Engineering

Objectives: Cell sources for cardiovascular tissue engineering (TE) are scant. However, the need for an ideal TE cardiovascular implant persists. We investigated the cardiotomy reservoir (CR) as a potential cell source that is more accessible and less ethically problematic. Methods: CR (n = 10) were removed from the bypass system after surgery. Isolation was performed using different isolation methods: blood samples were taken from the cardiopulmonary bypass and centrifuged at low density. The venous filter screen was cut out and placed into petri dishes for cultivation. The spongelike filter was removed, washed and treated in the same way as the blood samples. After cultivation, cell lines of fibroblasts (FB) and endothelial cells (EC) were obtained for analysis. The cells were seeded on polyurethane patches and analyzed via scanning electron microscopy (SEM), Life/Dead assay and immunohistochemistry. Results: No correlation between age, time of surgery and quality of cells was observed. The successful extraction of FB and was proven by positive staining results for TE-7, CD31 and vWF. Cell morphology, cytoskeleton staining and quantification of proliferation using WST-1 assay resembled the cells of the control group in all ways. The topography of a confluent and vital cell layer after cell seeding was displayed by SEM analysis, Life/Dead Assay and immunohistochemistry. The establishment of an extracellular matrix (ECM) was proven by positive staining for collagen IV, laminin, fibronectin and elastin. Conclusions: Viable FB and EC cell lines were extracted from the CR after surgery. Easy access and high availability make this cell source destined for widespread application in cardiovascular tissue engineering.

scholarly journals Human umbilical cord cells: a new cell source for cardiovascular tissue engineering

2002 ◽  
Vol 74 (4) ◽  
pp. 1422-1428 ◽  
Author(s):  
Alexander Kadner ◽  
Simon P Hoerstrup ◽  
Jay Tracy ◽  
Christian Breymann ◽  
C.hristine F Maurus ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Umbilical Cord ◽  
Human Umbilical Cord ◽  
Cardiovascular Tissue ◽  
Cell Source ◽  
Cardiovascular Tissue Engineering

Regenerative implants for cardiovascular tissue engineering

2014 ◽  
Vol 163 (4) ◽  
pp. 321-341 ◽  
Author(s):  
Avione Y. Lee ◽  
Nathan Mahler ◽  
Cameron Best ◽  
Yong-Ung Lee ◽  
Christopher K. Breuer
Keyword(s):  
Tissue Engineering ◽  
Cardiovascular Tissue ◽  
Cardiovascular Tissue Engineering
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