Docosahexaenoic acid is a potent inducer of apoptosis in HT-29 colon cancer cells

2000 ◽  
Vol 63 (5) ◽  
pp. 301-308 ◽  
Author(s):  
Z.Y. Chen ◽  
N.W. Istfan
Keyword(s):  
Colon Cancer ◽  
Docosahexaenoic Acid ◽  
Cancer Cells ◽  
Colon Cancer Cells ◽  
Potent Inducer ◽  
Ht 29

scholarly journals A Schiff Base-Derived Copper (II) Complex Is a Potent Inducer of Apoptosis in Colon Cancer Cells by Activating the Intrinsic Pathway

2014 ◽  
Vol 2014 ◽  
pp. 1-12 ◽  
Author(s):  
Maryam Hajrezaie ◽  
Mohammadjavad Paydar ◽  
Soheil Zorofchian Moghadamtousi ◽  
Pouya Hassandarvish ◽  
Nura Suleiman Gwaram ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Schiff Base ◽  
Cancer Cells ◽  
Chemotherapeutic Agents ◽  
Antiproliferative Effect ◽  
Great Promise ◽  
Potential Candidate ◽  
Colon Cancer Cells ◽  
Potent Inducer ◽  
Ht 29

Metal-based drugs with extensive clinical applications hold great promise for the development of cancer chemotherapeutic agents. In the last few decades, Schiff bases and their complexes have become well known for their extensive biological potential. In the present study, we examined the antiproliferative effect of a copper (II) complex on HT-29 colon cancer cells. The Cu(BrHAP)2Schiff base compound demonstrated a potent antiproliferative effect in HT-29 cells, with an IC50value of 2.87 μg/ml after 72 h of treatment. HT-29 cells treated with Cu (II) complexes underwent apoptosis death, as exhibited by a progressive elevation in the proportion of the G1cell population. At a concentration of 6.25 μg/ml, the Cu(BrHAP)2compound caused significant elevation in ROS production following perturbation of mitochondrial membrane potential and cytochromecrelease, as assessed by the measurement of fluorescence intensity in stained cells. Furthermore, the activation of caspases 3/7 and 9 was part of the Cu (II) complex-induced apoptosis, which confirmed the involvement of mitochondrial-mediated apoptosis. Meanwhile, there was no significant activation of caspase-8. Taken together, these results imply that the Cu(BrHAP)2compound is a potential candidate for furtherin vivoand clinical colon cancer studies to develop novel chemotherapeutic agents derived from metal-based agents.

scholarly journals Pro-Apoptotic Activity of Artichoke Leaf Extracts in Human HT-29 and RKO Colon Cancer Cells

2021 ◽  
Vol 18 (8) ◽  
pp. 4166
Author(s):  
Milena Villarini ◽  
Mattia Acito ◽  
Raffaella di Vito ◽  
Samuele Vannini ◽  
Luca Dominici ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Colon Cancer ◽  
Chlorogenic Acid ◽  
Cancer Cells ◽  
Herbaceous Plant ◽  
Colon Cancer Cells ◽  
Leaf Extracts ◽  
Caffeoylquinic Acids ◽  
Induction Of Apoptosis ◽  
Ht 29

(1) Background: Cynara cardunculus L. subsp. scolymus (L.) Hegi, popularly known as artichoke, is an herbaceous plant belonging to the Asteraceae family. Artichoke leaf extracts (ALEs) have been widely used in traditional medicine because of their hepatoprotective, cholagogic, hypoglycaemic, hypolipemic and antibacterial properties. ALEs are also recognized for their antioxidative and anti-inflammatory activities. In this study, we evaluated the cytotoxic, genotoxic, and apoptotic activities, as well as effect on cell growth of ALEs on human colon cancer HT-29 and RKO cells. HT-29 and RKO cells exhibit a different p53 status: RKO cells express the wild-type protein, whereas HT-29 cells express a p53-R273H contact mutant. (2) Methods: Four different ALEs were obtained by sequential extraction of dried artichoke leaves; ALEs were characterized for their content in chlorogenic acid, cynaropicrin, and caffeoylquinic acids. HT-29 and RKO cells were used for in vitro testing (i.e., cytotoxicity and genotoxicity assessment, cell cycle analysis, apoptosis induction). (3) Results: Two out of the four tested ALEs showed marked effects on cell vitality toward HT-29 and RKO tumour cells. The effect was accompanied by a genotoxic activity exerted at a non-cytotoxic concentrations, by a significant perturbation of cell cycle (i.e., with increase of cells in the sub-G1 phase), and by the induction of apoptosis. (4) Conclusions: ALEs rich in cynaropicrin, caffeoylquinic acids, and chlorogenic acid showed to be capable of affecting HT-29 and RKO colon cancer cells by inducing favourable biological effects: cell cycle perturbation, activation of mitochondrial dependent pathway of apoptosis, and the induction of genotoxic effects probably mediated by the induction of apoptosis. Taken together, these results weigh in favour of a potential cancer chemotherapeutic activity of ALEs.

Exploring the Colonic Metabolism of Grape and Strawberry Anthocyanins and Their in Vitro Apoptotic Effects in HT-29 Colon Cancer Cells

2016 ◽  
Vol 65 (31) ◽  
pp. 6477-6487 ◽  
Author(s):  
María-Carmen López de las Hazas ◽  
Juana I. Mosele ◽  
Alba Macià ◽  
Iziar A. Ludwig ◽  
María-José Motilva
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Colon Cancer Cells ◽  
Apoptotic Effects ◽  
Ht 29 ◽  
Colonic Metabolism

A glycoprotein from Laminaria japonica induces apoptosis in HT-29 colon cancer cells

2010 ◽  
Vol 24 (6) ◽  
pp. 1546-1553 ◽  
Author(s):  
Hiroe Go ◽  
Hye-Jung Hwang ◽  
Taek-Jeong Nam
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Laminaria Japonica ◽  
Colon Cancer Cells ◽  
Ht 29

scholarly journals Underlying mechanism for the modulation of apoptosis induced by a new benzoindole derivative on HT-29 colon cancer cells

RSC Advances ◽  
2017 ◽  
Vol 7 (61) ◽  
pp. 38257-38263 ◽  
Author(s):  
Fatemeh Hajiaghaalipour ◽  
Elham Bagheri ◽  
Fadhil Lafta Faraj ◽  
Mahmood Ameen Abdulla ◽  
Nazia Abdul Majid
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Underlying Mechanism ◽  
Colon Cancer Cells ◽  
Ht 29

DBID compound induced LDH leakage in HT-29 cells when compared to untreated cells.

Sign in / Sign up

Export Citation Format

Share Document