Impaired brain development and hydrocephalus in a line of transgenic mice with liver-specific expression of human insulin-like growth factor binding protein-1

2000 ◽  
Vol 10 (5) ◽  
pp. 267-274 ◽  
Author(s):  
S. Doublier ◽  
C. Duyckaerts ◽  
D. Seurin ◽  
M. Binoux
Keyword(s):  
Growth Factor ◽  
Transgenic Mice ◽  
Brain Development ◽  
Human Insulin ◽  
Binding Protein ◽  
Insulin Like Growth Factor ◽  
Specific Expression ◽  
Factor Binding

scholarly journals Functional Alteration of the Somatotrophic Axis in Transgenic Mice with Liver-Specific Expression of Human Insulin-like Growth Factor Binding Protein-1

2002 ◽  
Vol 52 (2) ◽  
pp. 168-174 ◽  
Author(s):  
Danielle Seurin ◽  
Pascal Froment ◽  
Marie-Thérèse Bluet-Pajot ◽  
Jacques Epelbaum ◽  
Philippe Monget ◽  
...  
Keyword(s):  
Growth Factor ◽  
Transgenic Mice ◽  
Human Insulin ◽  
Binding Protein ◽  
Insulin Like Growth Factor ◽  
Specific Expression ◽  
Factor Binding ◽  
Functional Alteration ◽  
Somatotrophic Axis

scholarly journals Reproductive Abnormalities in Human Insulin-Like Growth Factor-Binding Protein-1 Transgenic Male Mice

Endocrinology ◽  
2004 ◽  
Vol 145 (4) ◽  
pp. 2080-2091 ◽  
Author(s):  
Pascal Froment ◽  
Christophe Staub ◽  
Stéphanie Hembert ◽  
Claudine Pisselet ◽  
Michèle Magistrini ◽  
...  
Keyword(s):  
Growth Factor ◽  
Transgenic Mice ◽  
Human Insulin ◽  
Binding Protein ◽  
Reproductive Capacity ◽  
Insulin Like Growth Factor ◽  
Male Mice ◽  
Sperm Production ◽  
Factor Binding ◽  
Transgenic Male

Abstract Adult transgenic mice overexpressing human insulin-like growth factor-binding protein-1 in the liver present reproductive abnormalities in both sexes. In the present work, we have investigated the mechanisms responsible for limiting breeding capacity in these transgenic male mice. Homozygous adult transgenic male mice (3–6 months old) exhibited irregular copulatory behavior and a reduction of the number of pregnancies per female as well as of litter size per pregnancy. Genital tract weight, more specifically epididymal and seminal vesicle weights, were reduced by 45% in homozygous transgenic vs. nontransgenic mice. Homozygous transgenic mice exhibited a 30% reduction of the length of seminiferous tubules (P = 0.007), a 30% decrease in daily sperm production per testis (P = 0.019), and a 50% decrease in the number of spermatozoa in testis (P = 0.037), associated with morphological abnormalities of the sperm heads leading to an approximately 50% reduction of fertilized two-cell eggs (P = 0.002) and of implanted embryos on d 5.5 after mating (P = 0.004). The round spermatids also appeared altered in their morphology. In addition, Leydig cells in homozygous transgenic mice exhibited an altered appearance, with a 1.8-fold increase in lipid droplets in their cytoplasm (P < 0.001). Moreover, the concentration of 3β-hydroxysteroid dehydrogenase was 66% lower in testis from transgenics compared with those from normal mice (P = 0.01), leading to a tendency toward lower plasma testosterone levels (P = 0.1). Interestingly, LH concentrations were increased by 40% in transgenic pituitary extracts (P = 0.02), and basal LH secretion by pituitary explants in vitro was increased by 60% in homozygous transgenic vs. normal mice (P = 0.04), suggesting an alteration of LH pulsatile secretion in vivo. In conclusion, these data suggest that the breeding impairment of human insulin-like growth factor-binding protein-1 transgenic males is due at least in part to an alteration of the process of spermatogenesis, leading to a diminution of sperm production and of its quality. Minor impairment of steroidogenesis may also contribute to the reduced reproductive capacity of these animals. Our observations are consistent with the idea that normal spermatogenesis and perhaps also steroidogenesis are dependent on the actions of sufficient concentrations of unbound IGF-I.

Osteoblast-specific expression of insulin-like growth factor-1 in bone of transgenic mice induces insulin-like growth factor binding protein-5

Bone ◽  
2005 ◽  
Vol 36 (2) ◽  
pp. 224-231 ◽  
Author(s):  
Meilan M. Rutter ◽  
Edith Markoff ◽  
Lisa Clayton ◽  
Nagako Akeno ◽  
Guisheng Zhao ◽  
...  
Keyword(s):  
Growth Factor ◽  
Transgenic Mice ◽  
Binding Protein ◽  
Insulin Like Growth Factor ◽  
Specific Expression ◽  
Factor Binding

scholarly journals Insulin-Like Growth Factor Binding Protein-6 Transgenic Mice: Postnatal Growth, Brain Development, and Reproduction Abnormalities

Endocrinology ◽  
2004 ◽  
Vol 145 (5) ◽  
pp. 2412-2420 ◽  
Author(s):  
Géraldine Bienvenu ◽  
Danielle Seurin ◽  
Pascale Grellier ◽  
Pascal Froment ◽  
Marielle Baudrimont ◽  
...  
Keyword(s):  
Growth Factor ◽  
Transgenic Mice ◽  
Brain Development ◽  
Binding Protein ◽  
Postnatal Growth ◽  
Insulin Like Growth Factor ◽  
Factor Binding

scholarly journals Characterization of the chromosomal gene and promoter for human insulin-like growth factor binding protein-5.

1994 ◽  
Vol 269 (14) ◽  
pp. 10891-10898
Author(s):  
S.V. Allander ◽  
C. Larsson ◽  
E. Ehrenborg ◽  
A. Suwanichkul ◽  
G. Weber ◽  
...  
Keyword(s):  
Growth Factor ◽  
Human Insulin ◽  
Binding Protein ◽  
Insulin Like Growth Factor ◽  
Chromosomal Gene ◽  
Factor Binding
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