Functional characterization of thapsigargin and agonist-insensitive acidic Ca2+stores in Drosophila melanogaster S2 cell lines

Cell Calcium ◽  
1999 ◽  
Vol 25 (6) ◽  
pp. 429-438 ◽  
Author(s):  
S. Yagodin ◽  
N.B. Pivovarova ◽  
S.B. Andrews ◽  
D.B. Sattelle
Keyword(s):  
Drosophila Melanogaster ◽  
Cell Lines ◽  
Functional Characterization

scholarly journals NFB-01. FUNCTIONAL CHARACTERIZATION OF ATRX LOSS IN NF1-ASSOCIATED GLIOMA AND MPNST

2020 ◽  
Vol 22 (Supplement_3) ◽  
pp. iii417-iii418
Author(s):  
Ming Yuan ◽  
Karlyne Reilly ◽  
Christine Pratilas ◽  
Christopher Heaphy ◽  
Fausto Rodriguez
Keyword(s):  
Cell Lines ◽  
Functional Characterization ◽  
Tert Promoter ◽  
Aggressive Tumors ◽  
Sirna Knockdown ◽  
Nih 3T3 ◽  
Vitro Effect ◽  
Murine Glioma

Abstract To identify the biologic relevance of ATRX loss in NF1-associated gliomagenesis, we studied the effects of Atrx loss using four previously characterized Nf1+/-Trp53+/- murine glioma lines. Lines 130G#3 and 158D#8 (corresponding to grade IV and III gliomas, respectively) displayed preserved ATRX protein expression compared to NIH-3T3 cells. We studied the effects of Atrx knockdown in these two lines in the presence and absence of the TERT inhibitor, BIRBR1532. Using a telomere-specific FISH assay, we identified increased signal intensity after Atrx knockdown, only in the presence of the TERT inhibitor. These features are reminiscent of ALT, although there were no significant alterations in cell growth. Next, we studied the effect of ATRX loss in MPNST lines ST88-14, NF90-8, STS-26T. These cell lines all expressed ATRX and DAXX. However, STS-26T contained a TERT promoter mutation and ST88-14 had a known SNP in the TERT promoter, while NF90-8 had no alterations. ATRX siRNA knockdown showed no significant effects in cell proliferation or apoptosis. However, ATRX knockdown resulted in rare ultra-bright foci, indicative of ALT. Next, we studied the in vitro effect of the ATR inhibitor VE-821 in MPNST cell lines. Only NF90-8 (lacking TERT alterations) demonstrated a decrease in growth after ATRX knockdown and VE-821 treatment. However, ATRX knockdown alone did not affect sensitivity to carboplatin. Our findings further support a role for ATRX loss with subsequent ALT activation in a biologic subset of NF1-associated malignancies, thereby opening an opportunity for therapeutic targeting of these aggressive tumors using specific classes of drugs.

Functional characterization of HLA-DRA11 0101/ DRB1* 0401 molecules expressed in Drosophila melanogaster cells

2008 ◽  
Vol 51 (2) ◽  
pp. 119-128 ◽  
Author(s):  
Bjarke Endel Hansen ◽  
Ellen Christina Andersson ◽  
Lars Siim Madsen ◽  
Jan Engberg ◽  
Leif Søndergaard ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Functional Characterization

In vitro phenotypic and functional characterization of human pigment epithelial cell lines

1989 ◽  
Vol 8 (5) ◽  
pp. 435-440 ◽  
Author(s):  
Kamla Dutt ◽  
J. Clifford Waldrep ◽  
Henry J. Kaplan ◽  
Monte Del Monte ◽  
Eugene Semple ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Lines ◽  
Functional Characterization ◽  
Pigment Epithelial ◽  
Epithelial Cell Lines

scholarly journals Functional characterization of Drosophila melanogaster PERK eukaryotic initiation factor 2alpha (eIF2alpha) kinase

2003 ◽  
Vol 270 (2) ◽  
pp. 293-306 ◽  
Author(s):  
Natalia Pomar ◽  
Juan J. Berlanga ◽  
Sonsoles Campuzano ◽  
Greco Hernandez ◽  
Monica Elias ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Functional Characterization ◽  
Initiation Factor ◽  
Eukaryotic Initiation Factor ◽  
Eif2alpha Kinase

scholarly journals Functional characterization of osteosarcoma cell lines provides representative models to study the human disease

2011 ◽  
Vol 91 (8) ◽  
pp. 1195-1205 ◽  
Author(s):  
Alexander B Mohseny ◽  
Isidro Machado ◽  
Yongping Cai ◽  
Karl-Ludwig Schaefer ◽  
Massimo Serra ◽  
...  
Keyword(s):  
Cell Lines ◽  
Human Disease ◽  
Functional Characterization ◽  
Osteosarcoma Cell

scholarly journals Functional characterization of DcR3 in EBV transformed cell lines from IBD patients of different allelic background and role in disease pathogenesis

2019 ◽  
Vol 4 (2) ◽  
Author(s):  
Rahul Pandey ◽  
Christopher J Cardinale ◽  
Kelly Kachelries ◽  
Struan FA Grant ◽  
Robert N Baldassano ◽  
...  
Keyword(s):  
Cell Lines ◽  
Functional Characterization ◽  
Disease Pathogenesis ◽  
Transformed Cell ◽  
Transformed Cell Lines
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