scholarly journals Differential regulation of cell proliferation and protease secretion by epidermal growth factor and amphiregulin in tumoral versus normal breast epithelial cells

2001 ◽  
Vol 84 (7) ◽  
pp. 936-945 ◽  
Author(s):  
M Silvy ◽  
C Giusti ◽  
P-M Martin ◽  
Y Berthois
Keyword(s):  
Cell Proliferation ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Epithelial Cells ◽  
Normal Breast ◽  
Differential Regulation ◽  
Breast Epithelial Cells ◽  
Epidermal Growth ◽  
Protease Secretion ◽  
Normal Breast Epithelial Cells

Characterization of normal breast epithelial cells in primary cultures: Differentiation and growth factor receptors studies

1992 ◽  
Vol 28 (11-12) ◽  
pp. 716-724 ◽  
Author(s):  
Philippe Berthon ◽  
Gianfranco Pancino ◽  
Patricia Cremoux ◽  
Alberto Roseto ◽  
Christian Gespach ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epithelial Cells ◽  
Primary Cultures ◽  
Growth Factor Receptors ◽  
Normal Breast ◽  
Breast Epithelial Cells ◽  
Breast Epithelial ◽  
Normal Breast Epithelial Cells

Human chorionic gonadotropin and a 15 amino acid hCG fragment of the hormone induce downregulation of the cytokine IL-8 receptor in normal breast epithelial cells

2011 ◽  
Vol 6 (3) ◽  
Author(s):  
Samuel M.R. Noronha ◽  
Silvana A.A. Correa-Noronha ◽  
Irma H. Russo ◽  
Ricardo López de Cicco ◽  
Julia Santucci-Pereira ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Amino Acid ◽  
Epithelial Cells ◽  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Normal Breast ◽  
Breast Cancer Cell Lines ◽  
Breast Epithelial Cells ◽  
Breast Epithelial ◽  
Normal Breast Epithelial Cells

AbstractCytokine receptors are associated with tumor cell growth by increasing proliferation, metastasis and regulating self-renewal of cancer stem cells (SCs). There is a strong association between cytokine IL-8 receptor (CXCR1) over-expression and cells displaying SC characteristics. Human chorionic gonadotropin (hCG) causes differentiation, inhibition of cell proliferation and increased apoptosis of the breast epithelium. hCG receptor (LHCGR) expression in breast tumors and in breast cancer cell lines is undetectable or low. In this study, our objective was to assess and compare the effects of hCG and a 15 amino acid hCG fragment of the hormone on mRNA expression of CXCR1 and LHCGR on normal breast epithelial cells (MCF-10F) by real time RT-PCR after treatment with hCG or a hCG fragment for 15 days. Cell proliferation was also measured. hCG and the hCG fragment decreased cell proliferation in both groups. The compounds upregulated LHCGR expression and downregulated CXCR1 expression. It is possible to postulate that an increase of LHCGR mRNA seems to respond to the decrease of CXCR1 expression. These genes probably act synergistically to reduce the amount of cancer SCs in the mammary gland. Thereby, the use of hCG or the hCG fragment as a therapeutic or preventive tool should be considered.

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