scholarly journals Hepatocyte growth factor/scatter factor enhances the invasion of mesothelioma cell lines and the expression of matrix metalloproteinases

2000 ◽  
Vol 83 (9) ◽  
pp. 1147-1153 ◽  
Author(s):  
P Harvey ◽  
I M Clark ◽  
M-C Jaurand ◽  
R M Warn ◽  
D R Edwards
Keyword(s):  
Growth Factor ◽  
Matrix Metalloproteinases ◽  
Hepatocyte Growth Factor ◽  
Cell Lines ◽  
Scatter Factor ◽  
Mesothelioma Cell ◽  
Hepatocyte Growth

scholarly journals Concomitant Expression of Hepatocyte Growth Factor/Scatter Factor and the Receptor c-MET in Human Myeloma Cell Lines

1996 ◽  
Vol 271 (40) ◽  
pp. 24655-24661 ◽  
Author(s):  
Magne Børset ◽  
Egil Lien ◽  
Terje Espevik ◽  
Eirik Helseth ◽  
Anders Waage ◽  
...  
Keyword(s):  
Growth Factor ◽  
Hepatocyte Growth Factor ◽  
Cell Lines ◽  
Myeloma Cell ◽  
Scatter Factor ◽  
Hepatocyte Growth ◽  
Human Myeloma Cell

scholarly journals Neoexpression of the c-met/Hepatocyte Growth Factor-Scatter Factor Receptor Gene in Activated Monocytes

Blood ◽  
1997 ◽  
Vol 90 (11) ◽  
pp. 4450-4458 ◽  
Author(s):  
Mario Beilmann ◽  
Margarete Odenthal ◽  
Waltraud Jung ◽  
George F. Vande Woude ◽  
Hans-Peter Dienes ◽  
...  
Keyword(s):  
Growth Factor ◽  
Hepatocyte Growth Factor ◽  
Cell Lines ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Cell Function ◽  
Myeloid Cell ◽  
Scatter Factor ◽  
Activated Monocytes ◽  
Hepatocyte Growth

Hepatocyte growth factor-scatter factor (HGF-SF ) mediates mito-, moto-, and morphogenic effects through the MET receptor, a membrane bound tyrosine kinase. HGF-SF/MET signaling is mitogenic for a large number of epithelial and endothelial cells and activates organ regeneration. HGF-SF transcripts have been detected in various myeloid cell lines. Therefore, the potential role of HGF-SF/MET signaling for circulating cells of the immune system, especially under conditions of inflammation, was evaluated. Several B-lymphoid and myeloid cell lines were found to express HGF-SF or c-met transcripts, while activity of both genes was mutually exclusive with the exception of low level coexpression in two B-cell lines. HGF-SF transcripts were present in low quantities in freshly isolated peripheral blood mononuclear cells (PBMNCs). In contrast, c-met expression was not detected in freshly isolated cells from peripheral blood, but was induced in monocytes by activation of monocytic or T-cell function. HGF-SF incubation led to an increased c-fos steady state transcript level in myeloblastic K562 cells and moderately promoted cell viability of freshly isolated preactivated monocytes. c-met expression is thus established in activated monocytes, in particular under conditions resembling inflammation, making these cells accessible to functional effects of HGF-SF.

scholarly journals Neoexpression of the c-met/Hepatocyte Growth Factor-Scatter Factor Receptor Gene in Activated Monocytes

Blood ◽  
1997 ◽  
Vol 90 (11) ◽  
pp. 4450-4458 ◽  
Author(s):  
Mario Beilmann ◽  
Margarete Odenthal ◽  
Waltraud Jung ◽  
George F. Vande Woude ◽  
Hans-Peter Dienes ◽  
...  
Keyword(s):  
Growth Factor ◽  
Hepatocyte Growth Factor ◽  
Cell Lines ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Cell Function ◽  
Myeloid Cell ◽  
Scatter Factor ◽  
Activated Monocytes ◽  
Hepatocyte Growth

Abstract Hepatocyte growth factor-scatter factor (HGF-SF ) mediates mito-, moto-, and morphogenic effects through the MET receptor, a membrane bound tyrosine kinase. HGF-SF/MET signaling is mitogenic for a large number of epithelial and endothelial cells and activates organ regeneration. HGF-SF transcripts have been detected in various myeloid cell lines. Therefore, the potential role of HGF-SF/MET signaling for circulating cells of the immune system, especially under conditions of inflammation, was evaluated. Several B-lymphoid and myeloid cell lines were found to express HGF-SF or c-met transcripts, while activity of both genes was mutually exclusive with the exception of low level coexpression in two B-cell lines. HGF-SF transcripts were present in low quantities in freshly isolated peripheral blood mononuclear cells (PBMNCs). In contrast, c-met expression was not detected in freshly isolated cells from peripheral blood, but was induced in monocytes by activation of monocytic or T-cell function. HGF-SF incubation led to an increased c-fos steady state transcript level in myeloblastic K562 cells and moderately promoted cell viability of freshly isolated preactivated monocytes. c-met expression is thus established in activated monocytes, in particular under conditions resembling inflammation, making these cells accessible to functional effects of HGF-SF.

scholarly journals Ex Vivo Expansion of Human Pancreatic Endocrine Cells1

1997 ◽  
Vol 82 (6) ◽  
pp. 1852-1856
Author(s):  
Gillian M. Beattie ◽  
Vincenzo Cirulli ◽  
Ana D. Lopez ◽  
Alberto Hayek
Keyword(s):  
Growth Factor ◽  
Hepatocyte Growth Factor ◽  
Cell Lines ◽  
Islet Cells ◽  
Ex Vivo ◽  
Human Cell Line ◽  
Scatter Factor ◽  
Insulin Levels ◽  
Hepatocyte Growth

Abstract Cell transplantation as a therapy for type 1 diabetes is facilitated by ex vivo cell expansion of pancreatic β-cells without loss of differentiative characteristics. The aim of this study was to determine the optimal conditions for in vitro growth of functional human pancreatic endocrine tissue. We examined the mitogenicity of matrixes from a variety of cell lines; proliferation was greater in cells growing on matrixes from bladder carcinoma cell lines, especially in monolayers grown on matrix from the human cell line HTB-9. After 14-day culture, there was a more than 100-fold proliferative increase, which was augmented to a more than 200-fold when hepatocyte growth factor/scatter factor was added; however, hepatocyte growth factor/scatter factor induced a rapid decrease in insulin content. Without the growth factor, fetal cell monolayers expanded 4-fold with no insulin loss; however, after 12-fold expansion, the insulin levels decreased to 40% of those in unexpanded cells. Adult islet cells expanded 3-fold without insulin loss. After 5-fold expansion, insulin levels decreased by 25% compared to those in free floating islets while retaining a normal response to secretagogues. Together, these results indicate that HTB-9 matrix provides the best stimulatory effect on replication of human endocrine cells, with little loss of in vitro function.

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