scholarly journals Impact of cytokeratin-20 and carcinoembryonic antigen mRNA detection by RT-PCR in regional lymph nodes of patients with colorectal cancer

2000 ◽  
Vol 83 (10) ◽  
pp. 1323-1329 ◽  
Author(s):  
R Rosenberg ◽  
A Hoos ◽  
J Mueller ◽  
H Nekarda
Keyword(s):  
Colorectal Cancer ◽  
Lymph Nodes ◽  
Carcinoembryonic Antigen ◽  
Cytokeratin 20 ◽  
Rt Pcr ◽  
Regional Lymph Nodes ◽  
Mrna Detection

The potential of cytokeratin 20 and mucin 2 mRNA as metastasis markers in regional lymph nodes of colon cancer patients investigated by quantitative RT-PCR

2009 ◽  
Vol 24 (3) ◽  
pp. 261-268 ◽  
Author(s):  
Oddmund Nordgård ◽  
Satu Oltedal ◽  
Hartwig Kørner ◽  
Ole Gunnar Aasprong ◽  
Kjersti Tjensvoll ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Lymph Nodes ◽  
Cancer Patients ◽  
Cytokeratin 20 ◽  
Rt Pcr ◽  
Regional Lymph Nodes ◽  
Mucin 2

Prognostic Significance of Cytokeratin-20 Reverse Transcriptase Polymerase Chain Reaction in Lymph Nodes of Node-Negative Colorectal Cancer Patients

2002 ◽  
Vol 20 (4) ◽  
pp. 1049-1055 ◽  
Author(s):  
Robert Rosenberg ◽  
Axel Hoos ◽  
James Mueller ◽  
Patricia Baier ◽  
Dominik Stricker ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Overall Survival ◽  
Lymph Nodes ◽  
Prognostic Value ◽  
Tumor Recurrence ◽  
Cytokeratin 20 ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Node Negative ◽  
Polymerase Chain

PURPOSE: Approximately 20% to 30% of patients with curatively resected, node-negative (pN0) colorectal cancer die of tumor recurrence, which can be caused by minimal residual disease. To identify patients with an increased risk of tumor recurrence and evaluate the prognostic value of cytokeratin-20 (CK-20), we detected CK-20–positive cells in histopathologically tumor-free lymph nodes (pN0) of patients with colorectal cancer. PATIENTS AND METHODS: Two peritumoral lymph nodes each from 85 patients with completely resected (R0) colorectal cancer without lymph node metastases (pN0) by routine examination were analyzed using a CK-20–specific reverse transcriptase polymerase chain reaction (RT-PCR) and compared with CK-20–specific immunohistochemistry (IHC). The results were correlated with histopathologic findings and with survival. RESULTS: CK-20 RT-PCR was positive in 44 patients (52%) and detected 83% of cancer-related death. Positive RT-PCR was significantly correlated with poorer overall survival (P < .009). Comparing RT-PCR with IHC, 13 patients with positive RT-PCR were identified, where the CK-20 expression was caused by tumor cell contamination located exclusively outside the lymph node capsule and had no prognostic impact. Defining these 13 patients as RT-PCR negative improved specificity of the RT-PCR assay from 57% to 75%. The 5-year overall survival of the 31 RT-PCR–positive patients was 71%, compared with 96% in the 54 negative patients (P < .001). Multivariate analysis showed expression of CK-20 mRNA to be an independent prognostic factor with a relative risk of cancer-related death of 6.1. CONCLUSION: CK-20 RT-PCR in peritumoral histopathologic tumor-free (pN0) lymph nodes of colorectal cancer is an independent prognostic factor for overall survival. Additional CK-20 IHC improves the specificity and prognostic value of RT-PCR for cancer-related death.

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